CRISPR-associated endonuclease Cas9/Csn1
UniProt Q99ZW2 · gene cas9 · Streptococcus pyogenes serotype M1 · 1368 residues
161evidence items
0mapped to a state
0states
5techniques
4sources
0conflicts
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | xray | cryoem | nmr_shifts | force_spectroscopy | af_prediction | Total |
|---|---|---|---|---|---|---|---|
| No states defined yet. | |||||||
Unmapped evidence: 161 of 161 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 0 |
| BMRB | 1 | 0 |
| RCSB PDB | 141 | 0 |
| SMASH-BIO | 18 | 0 |
Structure
No state models are exported for this protein yet. Observed states export the representative PDB entry; inferred states need fitted models, not yet built.
States and transitions
No states defined.
| State | Basis | Parent | Description |
|---|
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-4UN4-2 RCSB PDB | xray | resolution: 2.371 Å | in vitro · -173.15 °C · pH 8.5 · ligands MG Crystal structure of Cas9 bound to PAM-containing DNA target with mismatches at positions 1-2 · CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B) | unmapped |
| EV-PDB-4UN5-2 RCSB PDB | xray | resolution: 2.4 Å | in vitro · -173.15 °C · pH 8.5 · ligands MG Crystal structure of Cas9 bound to PAM-containing DNA target containing mismatches at positions 1-3 · CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B) | unmapped |
| EV-PDB-4ZT0-1 RCSB PDB | xray | resolution: 2.9 Å | in vitro · -173.15 °C · pH 6 Crystal structure of catalytically-active Streptococcus pyogenes Cas9 in complex with single-guide RNA at 2.9 Angstrom resolution · CRISPR-associated endonuclease Cas9 (chains A,C) | unmapped |
| EV-PDB-4ZT9-1 RCSB PDB | xray | resolution: 3.1 Å | in vitro · -173.15 °C · pH 6 Nuclease-inactive Streptococcus pyogenes Cas9 (D10A/H840A, dCas9) in complex with single-guide RNA at 3.1 Angstrom resolution · CRISPR-associated endonuclease Cas9 (chains A,C) | unmapped |
| EV-PDB-6IFO-1 RCSB PDB | xray | resolution: 3.313 Å | in vitro · -173.15 °C · pH 6 Crystal structure of AcrIIA2-SpyCas9-sgRNA ternary complex · CRISPR-associated endonuclease Cas9/Csn1 (chains A,B) | unmapped |
| EV-PDB-6K4S-1 RCSB PDB | xray | resolution: 3.01 Å | in vitro · -188.15 °C · pH 8 Crystal structure of xCas9 in complex with sgRNA and DNA (TGC PAM) · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QQX-2 RCSB PDB | xray | resolution: 2.4 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG SpCas9 bound to FANCF off-target5 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QQW-2 RCSB PDB | xray | resolution: 3.1 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG SpCas9 bound to FANCF off-target4 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QQV-2 RCSB PDB | xray | resolution: 2.25 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG SpCas9 bound to FANCF off-target3 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QQU-2 RCSB PDB | xray | resolution: 2.45 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG SpCas9 bound to FANCF off-target2 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QQT-2 RCSB PDB | xray | resolution: 2.5 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG SpCas9 bound to FANCF off-target1 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QQS-2 RCSB PDB | xray | resolution: 2.4 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG SpCas9 bound to FANCF on-target DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QQR-2 RCSB PDB | xray | resolution: 2.75 Å | in vitro · -173.15 °C · pH 8.5 · ligands EDO, K, MG SpCas9 bound to AAVS1 off-target5 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QQQ-2 RCSB PDB | xray | resolution: 2.65 Å | in vitro · -173.15 °C · pH 8.5 · ligands EDO, K, MG SpCas9 bound to AAVS1 off-target4 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QQP-2 RCSB PDB | xray | resolution: 2.6 Å | in vitro · -173.15 °C · pH 8.5 · ligands EDO, K, MG SpCas9 bound to AAVS1 off-target3 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QQO-2 RCSB PDB | xray | resolution: 3 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG SpCas9 bound to AAVS1 off-target1 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7OXA-2 RCSB PDB | xray | resolution: 2.15 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG Target-bound SpCas9 complex with AAVS1 chimeric RNA-DNA guide · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7OX9-2 RCSB PDB | xray | resolution: 2.45 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG Target-bound SpCas9 complex with AAVS1 all-RNA guide · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7OX8-2 RCSB PDB | xray | resolution: 2.75 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG Target-bound SpCas9 complex with TRAC full RNA guide · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7OX7-2 RCSB PDB | xray | resolution: 2.6 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG Target-bound SpCas9 complex with TRAC chimeric RNA-DNA guide · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-6VPC-2 RCSB PDB | cryoem | resolution: 3.2 Å | in vitro · ligands MG, ZN Structure of the SpCas9 DNA adenine base editor - ABE8e · CRISPR-associated endonuclease Cas9 (chains B) | unmapped |
| EV-PDB-6O56-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · -173.15 °C · pH 6.5 HNH Nuclease from S. pyogenes Cas9 · CRISPR-associated endonuclease Cas9/Csn1 (chains A,B) | unmapped |
| EV-PDB-6O0Z-1 RCSB PDB | cryoem | resolution: 3.3 Å | in vitro Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-6O0Y-1 RCSB PDB | cryoem | resolution: 3.37 Å | in vitro Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-6O0X-1 RCSB PDB | cryoem | resolution: 3.28 Å | in vitro Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-6MCC-2 RCSB PDB | cryoem | resolution: 3.9 Å | in vitro CryoEM structure of AcrIIA2 homolog in complex with CRISPR-Cas9 · CRISPR-associated endonuclease Cas9 (chains A) | unmapped |
| EV-PDB-6K57-2 RCSB PDB | xray | resolution: 2.98 Å | in vitro · -188.15 °C · pH 8 Crystal structure of dCas9 in complex with sgRNA and DNA (CGA PAM) · CRISPR-associated endonuclease Cas9 (chains B) | unmapped |
| EV-PDB-6K4U-1 RCSB PDB | xray | resolution: 3.2 Å | in vitro · -188.15 °C · pH 8 Crystal structure of xCas9 in complex with sgRNA and DNA (TGA PAM) · CRISPR-associated endonuclease Cas9 (chains B) | unmapped |
| EV-PDB-6K4Q-4 RCSB PDB | xray | resolution: 2.7 Å | in vitro · -188.15 °C · pH 8 · ligands PO4 Crystal structure of xCas9 in complex with sgRNA and DNA (CGG PAM) · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-6K4P-4 RCSB PDB | xray | resolution: 2.9 Å | in vitro · -188.15 °C · pH 8 · ligands PO4 Crystal structure of xCas9 in complex with sgRNA and DNA (TGG PAM) · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-6K3Z-2 RCSB PDB | xray | resolution: 3.2 Å | in vitro · -188.15 °C · pH 8 Crystal structure of dCas9 in complex with sgRNA and DNA (TGA PAM) · CRISPR-associated endonuclease Cas9 (chains B) | unmapped |
| EV-PDB-6AI6-1 RCSB PDB | xray | resolution: 2.7 Å | in vitro · -173.15 °C · pH 8 · ligands EDO, K, MG Crystal structure of SpCas9-NG · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-6AEG-4 RCSB PDB | xray | resolution: 2.701 Å | in vitro · -173.15 °C Crystal structure of xCas9 in complex with sgRNA and target DNA (GAT PAM) · DNA nuclease (chains B) | unmapped |
| EV-PDB-6AEB-4 RCSB PDB | xray | resolution: 3.004 Å | in vitro · -173.15 °C Crystal structure of xCas9 in complex with sgRNA and target DNA (AAG PAM) · DNA Nuclease (chains B,F) | unmapped |
| EV-PDB-5Y36-1 RCSB PDB | cryoem | resolution: 5.2 Å | in vitro · ligands MG Cryo-EM structure of SpCas9-sgRNA-DNA ternary complex · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-5XBL-1 RCSB PDB | xray | resolution: 3.052 Å | in vitro · -173.15 °C Structure of nuclease in complex with associated protein · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-5VZL-2 RCSB PDB | cryoem | resolution: 3.9 Å | in vitro cryo-EM structure of the Cas9-sgRNA-AcrIIA4 anti-CRISPR complex · CRISPR-associated endonuclease Cas9 (chains A) | unmapped |
| EV-PDB-5VW1-1 RCSB PDB | xray | resolution: 2.598 Å | in vitro · -173.15 °C · pH 7.2 · ligands EDO, MG, PEG, PG4 Crystal structure of SpyCas9-sgRNA-AcrIIA4 ternary complex · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-5FW3-2 RCSB PDB | xray | resolution: 2.7 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG Crystal structure of SpCas9 variant VRER bound to sgRNA and TGCG PAM target DNA · CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B) | unmapped |
| EV-PDB-5FW2-2 RCSB PDB | xray | resolution: 2.676 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG Crystal structure of SpCas9 variant EQR bound to sgRNA and TGAG PAM target DNA · CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B) | unmapped |
| EV-PDB-5FW1-2 RCSB PDB | xray | resolution: 2.499 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG Crystal structure of SpyCas9 variant VQR bound to sgRNA and TGAG PAM target DNA · CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B) | unmapped |
| EV-PDB-5FQ5-2 RCSB PDB | xray | resolution: 2.136 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG Crystal structure of Cas9-sgRNA-DNA complex solved by native SAD phasing · CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B) | unmapped |
| EV-PDB-5F9R-1 RCSB PDB | xray | resolution: 3.4 Å | in vitro · -173.15 °C · pH 8 · ligands SO4 Crystal structure of catalytically-active Streptococcus pyogenes CRISPR-Cas9 in complex with single-guided RNA and double-stranded DNA primed for target DNA cleavage · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-5B2T-2 RCSB PDB | xray | resolution: 2.2 Å | in vitro · -173.15 °C · pH 8 · ligands ACT, EDO, K, MG Crystal structure of the Streptococcus pyogenes Cas9 VRER variant in complex with sgRNA and target DNA (TGCG PAM) · CRISPR-associated endonuclease Cas9 (chains B) | unmapped |
| EV-PDB-5B2S-2 RCSB PDB | xray | resolution: 2.2 Å | in vitro · -173.15 °C · pH 8 · ligands ACT, EDO, K, MG Crystal structure of the Streptococcus pyogenes Cas9 EQR variant in complex with sgRNA and target DNA (TGAG PAM) · CRISPR-associated endonuclease Cas9 (chains B) | unmapped |
| EV-PDB-5B2R-2 RCSB PDB | xray | resolution: 2 Å | in vitro · -173.15 °C · pH 8 · ligands ACT, EDO, K, MG Crystal structure of the Streptococcus pyogenes Cas9 VQR variant in complex with sgRNA and target DNA (TGA PAM) · CRISPR-associated endonuclease Cas9 (chains B) | unmapped |
| EV-PDB-4UN3-2 RCSB PDB | xray | resolution: 2.593 Å | in vitro · -173.15 °C · pH 8.5 · ligands MG Crystal structure of Cas9 bound to PAM-containing DNA target · CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B) | unmapped |
| EV-PDB-4OO8-1 RCSB PDB | xray | resolution: 2.5 Å | in vitro · -173.15 °C · pH 8 Crystal structure of Streptococcus pyogenes Cas9 in complex with guide RNA and target DNA · CRISPR-associated endonuclease Cas9/Csn1 (chains A,D) | unmapped |
| EV-PDB-4CMQ-1 RCSB PDB | xray | resolution: 3.09 Å | in vitro · -173.15 °C · pH 8.5 · ligands MN, SO4 Crystal structure of Mn-bound S.pyogenes Cas9 · CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains A,B) | unmapped |
| EV-PDB-4CMP-1 RCSB PDB | xray | resolution: 2.62 Å | in vitro · -173.15 °C · pH 8.5 · ligands MG, SO4 Crystal structure of S. pyogenes Cas9 · CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains A,B) | unmapped |
| EV-PDB-7QQZ-2 RCSB PDB | xray | resolution: 2.25 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG SpCas9 bound to FANCF off-target7 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QR1-2 RCSB PDB | xray | resolution: 2.6 Å | in vitro · -173.15 °C · ligands K, MG SpCas9 bound to TRAC off-target2 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QR5-2 RCSB PDB | xray | resolution: 3.3 Å | in vitro · -173.15 °C · pH 8.5 · ligands EDO, K, MG SpCas9 bound to FANCF off-target6 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QR7-2 RCSB PDB | xray | resolution: 3 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG SpCas9 bound to AAVS1 off-target2 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7S3H-2 RCSB PDB | cryoem | resolution: 2.5 Å | in vitro Cas9:sgRNA:DNA (S. pyogenes) with 0 RNA:DNA base pairs, open-protein/linear-DNA conformation · CRISPR-associated endonuclease Cas9/Csn1 (chains P) | unmapped |
| EV-PDB-7V59-1 RCSB PDB | cryoem | resolution: 5.26 Å | in vitro Cryo-EM structure of spyCas9-sgRNA-DNA dimer · CRISPR-associated endonuclease Cas9/Csn1 (chains B,E) | unmapped |
| EV-PDB-7ZO1-2 RCSB PDB | xray | resolution: 2.4 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG SpCas9 bound to CD34 off-target9 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-8KAG-1 RCSB PDB | xray | resolution: 3.88 Å | in vitro · -173.15 °C Crystal structure of SpyCas9 in complex with sgRNA and target RNA · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-8KAM-2 RCSB PDB | xray | resolution: 3.91 Å | in vitro · -173.15 °C Crystal structure of SpyCas9 in complex with sgRNA and 16nt target DNA · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-8SRS-1 RCSB PDB | cryoem | resolution: 2.79 Å | in vitro · ligands MG SpRY-Cas9:gRNA complex targeting TAC PAM DNA · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8T6X-1 RCSB PDB | cryoem | resolution: 3.08 Å | in vitro · ligands MG SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 18 bp R-loop · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8TZZ-1 RCSB PDB | cryoem | resolution: 3.56 Å | in vitro · ligands MG SpG Cas9 with NGC PAM DNA target · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8T7S-1 RCSB PDB | cryoem | resolution: 3.01 Å | in vitro · ligands MG SpRYmer bound to NAC PAM DNA · CRISPR-associated endonuclease Cas9/Csn1 (chains A,G) | unmapped |
| EV-PDB-8T79-1 RCSB PDB | cryoem | resolution: 3.04 Å | in vitro SpRY-Cas9:gRNA complex bound to non-target DNA with 10 bp R-loop · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8T78-1 RCSB PDB | cryoem | resolution: 3.02 Å | in vitro SpRY-Cas9:gRNA complex bound to non-target DNA with 8 bp R-loop · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8T77-1 RCSB PDB | cryoem | resolution: 3.25 Å | in vitro SpRY-Cas9:gRNA complex bound to non-target DNA with 6 bp R-loop · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8T76-1 RCSB PDB | cryoem | resolution: 3.42 Å | in vitro SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 3 bp R-loop · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8T6Y-1 RCSB PDB | cryoem | resolution: 2.88 Å | in vitro SpRY-Cas9:gRNA complex bound to non-target DNA with 1 bp R-loop · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8T6T-1 RCSB PDB | cryoem | resolution: 3.04 Å | in vitro SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 13 bp R-loop · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8T6S-1 RCSB PDB | cryoem | resolution: 3.28 Å | in vitro SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 10 bp R-loop · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8T6P-1 RCSB PDB | cryoem | resolution: 3.15 Å | in vitro SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 2 bp R-loop · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8T6O-2 RCSB PDB | cryoem | resolution: 3.1 Å | in vitro SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 0 bp R-loop · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8SQH-1 RCSB PDB | cryoem | resolution: 3.69 Å | in vitro · ligands MG SpRY-Cas9:gRNA complex targeting TTC PAM DNA · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8SPQ-1 RCSB PDB | cryoem | resolution: 3.26 Å | in vitro · ligands MG SpRY-Cas9:gRNA complex targeting TGG PAM DNA · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8SCA-1 RCSB PDB | xray | resolution: 1.67 Å | in vitro · -173.15 °C · ligands EDO Rec3 Domain from S. pyogenes Cas9 · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-8KAL-2 RCSB PDB | xray | resolution: 3.16 Å | in vitro · -173.15 °C · ligands SO4 Crystal structure of SpyCas9 in complex with sgRNA and 17nt target DNA · CRISPR-associated endonuclease Cas9/Csn1 (chains B,G) | unmapped |
| EV-PDB-8KAK-2 RCSB PDB | xray | resolution: 3.6 Å | in vitro · -173.15 °C · ligands SO4 Crystal structure of SpyCas9 in complex with sgRNA and 18nt target DNA · CRISPR-associated endonuclease Cas9/Csn1 (chains B,F) | unmapped |
| EV-PDB-8KAJ-2 RCSB PDB | xray | resolution: 3.42 Å | in vitro · -173.15 °C Crystal structure of SpyCas9-crRNA-tracrRNA complex bound to 16nt target DNA · CRISPR-associated endonuclease Cas9/Csn1 (chains B,F) | unmapped |
| EV-PDB-8KAI-2 RCSB PDB | xray | resolution: 3.49 Å | in vitro · -173.15 °C Crystal structure of SpyCas9-crRNA-tracrRNA complex bound to 17nt target DNA · CRISPR-associated endonuclease Cas9/Csn1 (chains B,F) | unmapped |
| EV-PDB-8KAH-2 RCSB PDB | xray | resolution: 3.36 Å | in vitro · -173.15 °C Crystal structure of SpyCas9-crRNA-tracrRNA complex bound to 18nt target DNA · CRISPR-associated endonuclease Cas9/Csn1 (chains B,F) | unmapped |
| EV-PDB-8G1I-2 RCSB PDB | cryoem | resolution: 3.12 Å | in vitro SpCas9 with sgRNA and target DNA · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8FZT-1 RCSB PDB | cryoem | resolution: 3.03 Å | in vitro SpCas9 with dual-guide RNA and target DNA · CRISPR-associated endonuclease Cas9/Csn1 (chains E) | unmapped |
| EV-PDB-7Z4L-2 RCSB PDB | cryoem | resolution: 2.54 Å | in vitro · ligands K, MG SpCas9 bound to 18-nucleotide complementary DNA substrate in the checkpoint state · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7Z4K-1 RCSB PDB | cryoem | resolution: 3.81 Å | in vitro SpCas9 bound to 10-nucleotide complementary DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7Z4J-5 RCSB PDB | cryoem | resolution: 2.99 Å | in vitro · ligands MG SpCas9 bound to 18-nucleotide complementary DNA substrate in the catalytic state · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7Z4I-4 RCSB PDB | cryoem | resolution: 3.12 Å | in vitro · ligands K SpCas9 bound to 16-nucleotide complementary DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7Z4H-4 RCSB PDB | cryoem | resolution: 3.49 Å | in vitro SpCas9 bound to 14-nucleotide complementary DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7Z4G-2 RCSB PDB | cryoem | resolution: 3.64 Å | in vitro SpCas9 bound to 12-nucleotide complementary DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7Z4E-1 RCSB PDB | cryoem | resolution: 4.14 Å | in vitro SpCas9 bound to 8-nucleotide complementary DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7Z4D-2 RCSB PDB | xray | resolution: 3.1 Å | in vitro · -173.15 °C · pH 6.5 · ligands K Crystal structure of SpCas9 bound to a 10 nucleotide complementary DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B,E) | unmapped |
| EV-PDB-7Z4C-4 RCSB PDB | cryoem | resolution: 3.87 Å | in vitro SpCas9 bound to 6 nucleotide complementary DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7VK9-1 RCSB PDB | xray | resolution: 2.9 Å | in vitro · -173.15 °C Crystal structure of xCas9 P411T · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-7S4X-1 RCSB PDB | cryoem | resolution: 2.76 Å | in vitro · ligands MG Cas9:gRNA in complex with 18-20MM DNA, 1 minute time-point, kinked active conformation · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-7S4V-1 RCSB PDB | cryoem | resolution: 3.28 Å | in vitro Cas9 bound to 12-14MM DNA, 60 min time-point, kinked conformation · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-7S4U-1 RCSB PDB | cryoem | resolution: 3.56 Å | in vitro Cryo-EM structure of Cas9 in complex with 12-14MM DNA substrate, 5 minute time-point · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-7S38-2 RCSB PDB | cryoem | resolution: 3.3 Å | in vitro Cas9:sgRNA:DNA (S. pyogenes) forming a 3-base-pair R-loop · CRISPR-associated endonuclease Cas9/Csn1 (chains P) | unmapped |
| EV-PDB-7S37-1 RCSB PDB | cryoem | resolution: 3.2 Å | in vitro Cas9:sgRNA (S. pyogenes) in the open-protein conformation · CRISPR-associated endonuclease Cas9/Csn1 (chains P) | unmapped |
| EV-PDB-7S36-2 RCSB PDB | cryoem | resolution: 3.2 Å | in vitro Cas9:sgRNA:DNA (S. pyogenes) with 0 RNA:DNA base pairs, closed-protein/bent-DNA conformation · CRISPR-associated endonuclease Cas9/Csn1 (chains P) | unmapped |
| EV-PDB-7QR8-2 RCSB PDB | xray | resolution: 2.75 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG SpCas9 bound to PTPRC off-target1 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-7QR0-2 RCSB PDB | xray | resolution: 2.3 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, MG SpCas9 bound to TRAC off-target1 DNA substrate · CRISPR-associated endonuclease Cas9/Csn1 (chains B) | unmapped |
| EV-PDB-8U3Y-1 RCSB PDB | cryoem | resolution: 3.1 Å | in vitro · ligands MG SpG Cas9 with NGG PAM DNA target · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8WUS-2 RCSB PDB | cryoem | resolution: 2.9 Å | in vitro SpCas9-MMLV RT-pegRNA-target DNA complex (termination) · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8WUV-3 RCSB PDB | cryoem | resolution: 3 Å | in vitro SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 16-nt) · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9CGU-1 RCSB PDB | cryoem | resolution: 3 Å | in vitro Cas9 ternary complex, 14-nt sgRNA, State II (kinked) · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9EAK-4 RCSB PDB | cryoem | resolution: 2.76 Å | in vitro · ligands MG SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State I - pre-activation) · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9EAL-4 RCSB PDB | cryoem | resolution: 2.83 Å | in vitro SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State II - precleavage) · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9ED9-5 RCSB PDB | cryoem | resolution: 2.95 Å | in vitro SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State III - checkpoint) · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9EDA-5 RCSB PDB | cryoem | resolution: 2.88 Å | in vitro SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State IV - product) · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9H4L-1 RCSB PDB | cryoem | resolution: 3.9 Å | in vitro dCas9 bound to off-target 2 EMX1-1 (-)SC DNA minicircle · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9H4M-4 RCSB PDB | cryoem | resolution: 3.8 Å | in vitro wtCas9 bound to off-target 1 EMX1-1 (-)SC DNA minicircle · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9JL4-1 RCSB PDB | cryoem | resolution: 4 Å | in vitro Apo-spCas9 conformation · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9K4C-1 RCSB PDB | cryoem | resolution: 3.84 Å | in vitro SuperFi Cas9 - 22nt sgRNA - DNA ternary complex · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9K4E-1 RCSB PDB | cryoem | resolution: 3.3 Å | in vitro SuperFi Cas9 - mismatch 22nt sgRNA - DNA ternary complex class1 · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9LKJ-2 RCSB PDB | cryoem | resolution: 2.68 Å | in vitro Structure of Cas9-sgRNA-A27 · CRISPR-associated endonuclease Cas9/Csn1 (chains C) | unmapped |
| EV-PDB-9W7T-1 RCSB PDB | cryoem | resolution: 3.91 Å | in vitro SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class B · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9W7U-1 RCSB PDB | cryoem | resolution: 3.83 Å | in vitro SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class C · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9W7V-1 RCSB PDB | cryoem | resolution: 3.81 Å | in vitro SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class D · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9WDX-1 RCSB PDB | cryoem | resolution: 3.72 Å | in vitro Structure of SpCas9 with engineered disulfide bonds (R1210C/V1280C and L1119C/P1128C) · CRISPR-associated endonuclease Cas9/Csn1 (chains P) | unmapped |
| EV-PDB-9ZQ6-1 RCSB PDB | cryoem | resolution: 2.98 Å | in vitro Structure of SpyCas9 in complex with the anti-CRISPR protein AcrIIA26 · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9WAW-1 RCSB PDB | cryoem | resolution: 3.95 Å | in vitro SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class E · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9WAA-1 RCSB PDB | cryoem | resolution: 4.17 Å | in vitro SuperFi Cas9 - 22nt sgRNA - DNA ternary complex Class C · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9WA9-1 RCSB PDB | cryoem | resolution: 4.12 Å | in vitro SuperFi Cas9 - 22nt sgRNA - DNA ternary complex Class B · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9W9D-1 RCSB PDB | cryoem | resolution: 4.4 Å | in vitro SuperFi Cas9 - 22nt sgRNA - DNA ternary complex · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9W7Q-1 RCSB PDB | cryoem | resolution: 3.79 Å | in vitro SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class A · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9KJ6-1 RCSB PDB | xray | resolution: 3.59 Å | in vitro · -173.15 °C · pH 7.5 Crystal Structure of SpCas9 ternary complex, amino acids (1242-1263) replaced with Gly-Ser linker · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9K4H-1 RCSB PDB | cryoem | resolution: 3.36 Å | in vitro SuperFi Cas9 - mismatch 22nt sgRNA - DNA ternary complex class3 · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9K4F-1 RCSB PDB | cryoem | resolution: 3.27 Å | in vitro SuperFi Cas9 - mismatch 22nt sgRNA - DNA ternary complex class2 · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9K4D-1 RCSB PDB | cryoem | resolution: 3.53 Å | in vitro SuperFi Cas9 - 20nt sgRNA - DNA ternary complex · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9JL2-3 RCSB PDB | cryoem | resolution: 3 Å | in vitro Classical conformation for the spCas9 with gRNA and target DNA complex · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9JL1-1 RCSB PDB | cryoem | resolution: 3.5 Å | in vitro SpCas9 with mini-gRNA conformation · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9H4K-1 RCSB PDB | cryoem | resolution: 2.6 Å | in vitro dCas9 bound to off-target 1 EMX1-1 (-)SC DNA minicircle · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9H4J-1 RCSB PDB | cryoem | resolution: 3.1 Å | in vitro dCas9 bound to on-target EMX1-1 (-)SC DNA minicircle · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9EDB-1 RCSB PDB | cryoem | resolution: 3.1 Å | in vitro SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State V - dissociated) · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9C9P-1 RCSB PDB | cryoem | resolution: 3.1 Å | in vitro Cas9 ternary complex, 14-nt sgRNA, State I (linear) · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-9B2K-3 RCSB PDB | cryoem | resolution: 3.67 Å | in vitro SpCas9 with dual-guide RNA in open conformation · CRISPR-associated endonuclease Cas9/Csn1 (chains P) | unmapped |
| EV-PDB-8YNY-9 RCSB PDB | cryoem | resolution: 4.52 Å | in vitro Structure of Cas9-sgRNA ribonucleoprotein bound to nucleosome · CRISPR-associated endonuclease Cas9/Csn1 (chains X) | unmapped |
| EV-PDB-8YGJ-6 RCSB PDB | cryoem | resolution: 3.2 Å | in vitro SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 28-nt) · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8YE9-1 RCSB PDB | cryoem | resolution: 3.43 Å | in vitro Cryo-EM structure of Cas9-sgRNA-A25 complex · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8YE6-2 RCSB PDB | cryoem | resolution: 2.95 Å | in vitro Cryo-EM structure of Cas9-sgRNA-A32 complex · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8WUU-1 RCSB PDB | cryoem | resolution: 3.2 Å | in vitro SpCas9-pegRNA-target DNA complex (pre-initiation) · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-PDB-8WUT-1 RCSB PDB | cryoem | resolution: 3 Å | in vitro SpCas9-MMLV RT-pegRNA-target DNA complex (initiation) · CRISPR-associated endonuclease Cas9/Csn1 (chains A) | unmapped |
| EV-BMRB-27949 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 7.4 1H, 13C, 15N Backbone and Sidechain Assignments of HNH from SpyCas9 · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-AFDB-AF-Q99ZW2-F1 AlphaFold DB | af_prediction | plddt: 88.38 pLDDT | in silico AlphaFold DB prediction AF-Q99ZW2-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-1368 | unmapped |
| EV-SMASH-SMFS-000161 SMASH-BIO | force_spectroscopy | study_record | in cell · pH 8 Leader cell myosin 10 controls adhesion dynamics, invasion, and EMT pathway activation. · Cas9 (S. pyogenes); tension sensors; experiment class: force_generation_motor, nucleic_acid_protein_interaction, receptor_ligand_adhesion, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001184 SMASH-BIO | force_spectroscopy | study_record | in vitro Huntingtin S421 phosphorylation increases kinesin and dynein engagement on early endosomes and lysosomes. · Cas9 (S. pyogenes); optical tweezers; experiment class: force_generation_motor, unfolding_protein | unmapped |
| EV-SMASH-SMFS-001258 SMASH-BIO | force_spectroscopy | study_record | in vitro Negative DNA supercoiling induces genome-wide Cas9 off-target activity. · Cas9 (S. pyogenes); optical tweezers; experiment class: nucleic_acid_mechanics, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001429 SMASH-BIO | force_spectroscopy | study_record | in vitro CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Microscopy. · Cas9 (S. pyogenes); optical tweezers; experiment class: nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-001550 SMASH-BIO | force_spectroscopy | study_record | in vitro Huntingtin S421 phosphorylation increases kinesin and dynein engagement on early endosomes and lysosomes · Cas9 (S. pyogenes); optical tweezers; experiment class: force_generation_motor, unfolding_protein | unmapped |
| EV-SMASH-SMFS-001877 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 Detection of genetic variation and base modifications at base-pair resolution on both DNA and RNA. · Cas9; magnetic tweezers; experiment class: nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-001943 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis. · Cas9 (S. pyogenes); optical tweezers; experiment class: nucleic_acid_mechanics, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-002021 SMASH-BIO | force_spectroscopy | study_record | in vitro Molecular mechanisms of Streptococcus pyogenes Cas9: a single-molecule perspective. · Cas9 (S. pyogenes); optical tweezers; experiment class: nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-002064 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.8 Oriented Soft DNA Curtains for Single-Molecule Imaging. · Cas9 (S. pyogenes); hydrodynamic stretching; experiment class: nucleic_acid_protein_interaction, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-002078 SMASH-BIO | force_spectroscopy | study_record | in vitro Probing the stability of the SpCas9-DNA complex after cleavage · Cas9 (S. pyogenes); magnetic tweezers; experiment class: nucleic_acid_mechanics, nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-002079 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Probing the stability of the SpCas9-DNA complex after cleavage. · Cas9 (S. pyogenes); optical tweezers; experiment class: nucleic_acid_mechanics, nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-002215 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 A Region of UNC-89 (Obscurin) Lying between Two Protein Kinase Domains Is a Highly Elastic Spring Required for Proper Sarcomere Organization. · Cas9 (S. pyogenes); afm smfs; experiment class: force_generation_motor, rheology_cell | unmapped |
| EV-SMASH-SMFS-002216 SMASH-BIO | force_spectroscopy | study_record | in vitro A Region of UNC-89 (obscurin) Lying Between Two Protein Kinase Domains is a Highly Elastic Spring Required for Proper Sarcomere Assembly · Cas9 (S. pyogenes); afm smfs; experiment class: force_generation_motor, rheology_cell | unmapped |
| EV-SMASH-SMFS-002482 SMASH-BIO | force_spectroscopy | study_record | in vitro Oriented Soft DNA Curtains for Single Molecule Imaging · Cas9 (S. pyogenes); hydrodynamic stretching; experiment class: nucleic_acid_protein_interaction, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-002676 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 DNA stretching induces Cas9 off-target activity. · Cas9 (S. pyogenes); optical tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-002930 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 8 The Effect of DNA Topology on Observed Rates of R-Loop Formation and DNA Strand Cleavage by CRISPR Cas12a. · Cas9 (S. pyogenes); magnetic tweezers; experiment class: nucleic_acid_mechanics, nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-003702 SMASH-BIO | force_spectroscopy | study_record | in vitro Single-Molecule Insight Into Target Recognition by CRISPR-Cas Complexes. · Cas9 (S. pyogenes); magnetic tweezers; experiment class: nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-004045 SMASH-BIO | force_spectroscopy | study_record | in vitro Single Molecule Localization and Discrimination of DNA-Protein Complexes by Controlled Translocation Through Nanocapillaries. · dCas9; optical tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
Conflicts
No conflicts found among comparable evidence.
161 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.