Vinculin
UniProt Q64727 · gene Vcl · Mus musculus · 1066 residues
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | xray | nmr_shifts | force_spectroscopy | af_prediction | Total |
|---|---|---|---|---|---|---|
| No states defined yet. | ||||||
Unmapped evidence: 14 of 14 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 0 |
| BMRB | 1 | 0 |
| RCSB PDB | 2 | 0 |
| SMASH-BIO | 10 | 0 |
Structure
No state models are exported for this protein yet. Observed states export the representative PDB entry; inferred states need fitted models, not yet built.
States and transitions
No states defined.
| State | Basis | Parent | Description |
|---|
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-5Y04-1 RCSB PDB | xray | resolution: 2.85 Å | in vitro · -173.15 °C · pH 8 Crystal Structure of the complex between the vinculin D1 domain and alphaE-catenin · Vinculin (chains A) | unmapped |
| EV-PDB-9SR0-1 RCSB PDB | xray | resolution: 1.49 Å | in vitro · -173.15 °C · pH 6 Mouse vinculin head domain 1 (VD1) (residues 2-258) with A50I mutation in complex with mouse talin 1 helix 50 (H50, residues 2072-2103) · Vinculin (chains A) | unmapped |
| EV-BMRB-15653 BMRB | nmr_shifts | chemical_shift | in vitro · 36.85 °C · pH 5.5 Backbone 1H, 13C, and 15N NMR Assignments of the Tail Domain of Vinculin · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-AFDB-AF-Q64727-F1 AlphaFold DB | af_prediction | plddt: 89.56 pLDDT | in silico AlphaFold DB prediction AF-Q64727-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-1066 | unmapped |
| EV-SMASH-SMFS-000345 SMASH-BIO | force_spectroscopy | study_record | in vitro WHaloForce enables chemigenetic imaging of molecular tension in living cells and animals · Vinculin; optical tweezers; experiment class: receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-001065 SMASH-BIO | force_spectroscopy | study_record | in vitro Biphasic reinforcement of nascent adhesions by vinculin. · Vinculin; optical tweezers; experiment class: receptor_ligand_adhesion, rheology_cell | unmapped |
| EV-SMASH-SMFS-004243 SMASH-BIO | force_spectroscopy | study_record | in vitro Imaging viscoelastic properties of live cells by AFM: power-law rheology on the nanoscale. · Vinculin; afm smfs; experiment class: receptor_ligand_adhesion, rheology_cell | unmapped |
| EV-SMASH-SMFS-004432 SMASH-BIO | force_spectroscopy | study_record | in vitro Vinculin phosphorylation at residues Y100 and Y1065 is required for cellular force transmission. · Vinculin; magnetic tweezers; experiment class: receptor_ligand_adhesion, rheology_cell, rheology_material | unmapped |
| EV-SMASH-SMFS-004869 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.6 Force mapping during the formation and maturation of cell adhesion sites with multiple optical tweezers. · Vinculin; optical tweezers; experiment class: receptor_ligand_adhesion, rheology_cell | unmapped |
| EV-SMASH-SMFS-005974 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 What is vinculin needed for in platelets? · Vinculin; optical tweezers; experiment class: force_generation_polymerization, membrane_mechanics, receptor_ligand_adhesion, rheology_cell | unmapped |
| EV-SMASH-SMFS-005991 SMASH-BIO | force_spectroscopy | study_record | in vitro Anchorage of vinculin to lipid membranes influences cell mechanical properties. · Vinculin; magnetic tweezers; experiment class: receptor_ligand_adhesion, rheology_cell | unmapped |
| EV-SMASH-SMFS-006009 SMASH-BIO | force_spectroscopy | study_record | in vitro Comparing the mechanical influence of vinculin, focal adhesion kinase and p53 in mouse embryonic fibroblasts. · Vinculin; magnetic tweezers; experiment class: force_generation_motor, receptor_ligand_adhesion, rheology_cell | unmapped |
| EV-SMASH-SMFS-006380 SMASH-BIO | force_spectroscopy | study_record | in vitro Mechano-coupling and regulation of contractility by the vinculin tail domain. · Vinculin; magnetic tweezers; experiment class: receptor_ligand_adhesion, rheology_cell, unfolding_protein | unmapped |
| EV-SMASH-SMFS-007470 SMASH-BIO | force_spectroscopy | study_record | in vitro Analysis of cell mechanics in single vinculin-deficient cells using a magnetic tweezer. · Vinculin; magnetic tweezers; experiment class: receptor_ligand_adhesion, rheology_cell | unmapped |
Conflicts
No conflicts found among comparable evidence.
14 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.