RecQ-like DNA helicase BLM
UniProt P54132 · gene BLM · Homo sapiens · 1417 residues
34evidence items
0mapped to a state
0states
5techniques
5sources
0conflicts
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | xray | nmr_structure | nmr_shifts | force_spectroscopy | af_prediction | Total |
|---|---|---|---|---|---|---|---|
| No states defined yet. | |||||||
Unmapped evidence: 34 of 34 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 0 |
| BMRB | 3 | 0 |
| DisProt | 5 | 0 |
| RCSB PDB | 17 | 0 |
| SMASH-BIO | 8 | 0 |
Structure
No state models are exported for this protein yet. Observed states export the representative PDB entry; inferred states need fitted models, not yet built.
States and transitions
No states defined.
| State | Basis | Parent | Description |
|---|
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-4CDG-1 RCSB PDB | xray | resolution: 2.794 Å | in vitro · -173.15 °C · ligands ADP, ZN Crystal structure of the Bloom's syndrome helicase BLM in complex with Nanobody · BLOOM SYNDROME PROTEIN (chains A,B) | unmapped |
| EV-PDB-5LUP-2 RCSB PDB | xray | resolution: 2.032 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, PO4 Structures of DHBN domain of human BLM helicase · BLM protein (chains H) | unmapped |
| EV-PDB-5LUP-1 RCSB PDB | xray | resolution: 2.032 Å | in vitro · -173.15 °C · pH 8.5 · ligands K, PO4 Structures of DHBN domain of human BLM helicase · BLM protein (chains A,B,C,D,E,F,G,I,J,K,L) | unmapped |
| EV-PDB-5MK5-1 RCSB PDB | xray | resolution: 2.16 Å | in vitro · -173.15 °C · pH 8.5 · ligands IOD, K Structures of DHBN domain of human BLM helicase · Bloom syndrome protein (chains A,B,C,D) | unmapped |
| EV-PDB-5U6K-2 RCSB PDB | xray | resolution: 2.6 Å | in vitro · 24.85 °C · pH 5.4 Crystal structure of TopBP1 BRCT4/5 in complex with a BLM phosphopeptide · Bloom Sydrome recQ helicase like protein (BLM) (chains L,M,N,O) | unmapped |
| EV-PDB-7AUD-1 RCSB PDB | xray | resolution: 2.96 Å | in vitro · -173.15 °C · pH 6.5 · ligands ADP, MG, PG4, RY8, ZN Structure of an engineered helicase domain construct for human Bloom syndrome protein (BLM) · Bloom syndrome protein,Bloom syndrome protein (chains A,B,C,D,E,F) | unmapped |
| EV-PDB-7XUW-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · -173.15 °C · pH 4.6 Crystal structure of RPA70N-BLMp2 fusion · fusion protein of Replication protein A 70 kDa DNA-binding subunit and Bloom syndrome protein (chains A) | unmapped |
| EV-PDB-7XV0-2 RCSB PDB | xray | resolution: 1.5 Å | in vitro · -173.15 °C · pH 5.6 Crystal structure of RPA70N-BLMp1 fusion · Bloom syndrome protein (chains B) | unmapped |
| EV-PDB-9P3E-1 RCSB PDB | xray | resolution: 2 Å | in vitro · -173.15 °C · ligands CL LC3B bound to BLM peptide · RecQ-like DNA helicase BLM,Microtubule-associated protein 1 light chain 3 beta (chains A) | unmapped |
| EV-PDB-7AUC-1 RCSB PDB | xray | resolution: 1.53 Å | in vitro · -173.15 °C · pH 7.5 · ligands ACT, ADP, CA, EDO, GOL, MG, PG4, ZN Crystal structure of an engineered helicase domain construct for human Bloom syndrome protein (BLM) · Bloom syndrome protein,Bloom syndrome protein (chains A) | unmapped |
| EV-PDB-4O3M-1 RCSB PDB | xray | resolution: 2.3 Å | in vitro · -173.15 °C · pH 6 · ligands ADP, CA, EDO, ZN Ternary complex of Bloom's syndrome helicase · Bloom syndrome protein (chains A) | unmapped |
| EV-PDB-4CGZ-1 RCSB PDB | xray | resolution: 3.2 Å | in vitro · -173.15 °C · ligands ADP, ZN Crystal structure of the Bloom's syndrome helicase BLM in complex with DNA · BLOOM'S SYNDROME HELICASE (chains A) | unmapped |
| EV-PDB-3WE3-1 RCSB PDB | xray | resolution: 2.9 Å | in vitro · -183.15 °C · pH 8.4 · ligands ACT, ART Structure of BLM RQC domain bound to an arsenate ion · Bloom syndrome protein (chains A,B) | unmapped |
| EV-PDB-3WE2-1 RCSB PDB | xray | resolution: 2.7 Å | in vitro · -183.15 °C · pH 8.4 · ligands ACT, PO4 Structure of BLM RQC domain bound to a phosphate ion · Bloom syndrome protein (chains A,B) | unmapped |
| EV-PDB-2RRD-1 RCSB PDB | nmr_structure | structure | in vitro · 29.85 °C · pH 7.5 Structure of HRDC domain from human Bloom syndrome protein, BLM · HRDC domain from Bloom syndrome protein (chains A) | unmapped |
| EV-PDB-2MH9-1 RCSB PDB | nmr_structure | structure | in vitro · 26.85 °C · pH 7 Resonance assignment of RQC domain of human Bloom syndrome protein · Bloom syndrome protein (chains A) | unmapped |
| EV-PDB-2KV2-1 RCSB PDB | nmr_structure | structure | in vitro · 24.85 °C · pH 7 Solution Structure of the human BLM HRDC domain · Bloom syndrome protein (chains A) | unmapped |
| EV-BMRB-11252 BMRB | nmr_shifts | chemical_shift | in vitro · 29.85 °C · pH 7.5 Resonance assignments of HRDC domain from Bloom syndrome protein · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-BMRB-16766 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 7 Solution Structure of the human BLM HRDC domain · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-BMRB-19530 BMRB | nmr_shifts | chemical_shift | in vitro · 26.85 °C · pH 7 Resonance assignment of RQC domain of human Bloom syndrome protein · BMRB data: 1H chemical shifts, 13C chemical shifts, 15N chemical shifts | unmapped |
| EV-DISPROT-DP03061r001 DisProt | xray | disorder_region: 14 residues | in vitro DisProt DP03061: disorder, residues 1093-1106 · residues 1093-1106; evidence: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion | unmapped |
| EV-DISPROT-DP03061r002 DisProt | nmr_shifts | disorder_region: 20 residues | in vitro DisProt DP03061: disorder, residues 1091-1110 · residues 1091-1110; evidence: nuclear magnetic resonance spectroscopy evidence used in manual assertion | unmapped |
| EV-DISPROT-DP03061r003 DisProt | xray | disorder_region: 11 residues | in vitro DisProt DP03061: disorder, residues 1004-1014 · residues 1004-1014; evidence: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion | unmapped |
| EV-DISPROT-DP03061r004 DisProt | xray | disorder_region: 16 residues | in vitro DisProt DP03061: disorder, residues 1092-1107 · residues 1092-1107; evidence: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion | unmapped |
| EV-DISPROT-DP03061r007 DisProt | xray | disorder_region: 13 residues | in vitro DisProt DP03061: disorder, residues 1195-1207 · residues 1195-1207; evidence: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion | unmapped |
| EV-AFDB-AF-P54132-F1 AlphaFold DB | af_prediction | plddt: 60.53 pLDDT | in silico AlphaFold DB prediction AF-P54132-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-1417 | unmapped |
| EV-SMASH-SMFS-000610 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Single-Molecule Visualization of BLM-DNA2-Mediated DNA End Resection Using DNA Curtains. · BLM helicase; hydrodynamic stretching; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001366 SMASH-BIO | force_spectroscopy | study_record | in vitro Understanding G-Quadruplex Biology and Stability Using Single-Molecule Techniques. · BLM helicase; optical tweezers; experiment class: nucleic_acid_protein_interaction, receptor_ligand_adhesion, translation_transcription_replication, unfolding_nucleic_acid, unfolding_protein | unmapped |
| EV-SMASH-SMFS-001487 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Duplex DNA and BLM regulate gate opening by the human TopoIIIα-RMI1-RMI2 complex. · BLM helicase; optical tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001707 SMASH-BIO | force_spectroscopy | study_record | in vitro Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding. · BLM helicase; optical tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-002639 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 8 Assembling the Human Resectosome on DNA Curtains. · BLM helicase; hydrodynamic stretching; experiment class: translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-002903 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Single-molecule visualization of human BLM helicase as it acts upon double- and single-stranded DNA substrates. · BLM helicase; hydrodynamic stretching; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-004875 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 8 Fork sensing and strand switching control antagonistic activities of RecQ helicases. · RecQ2; magnetic tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-004980 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 PICH: a DNA translocase specially adapted for processing anaphase bridge DNA. · BLM helicase; optical tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
Conflicts
No conflicts found among comparable evidence.
34 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.