Kinetochore protein NDC80
UniProt P40460 · gene NDC80 · Saccharomyces cerevisiae (strain ATCC 204508 / S288c) · 691 residues
31evidence items
0mapped to a state
0states
5techniques
4sources
0conflicts
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | xray | cryoem | nmr_shifts | force_spectroscopy | af_prediction | Total |
|---|---|---|---|---|---|---|---|
| No states defined yet. | |||||||
Unmapped evidence: 31 of 31 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 0 |
| BMRB | 1 | 0 |
| RCSB PDB | 9 | 0 |
| SMASH-BIO | 20 | 0 |
Structure
No state models are exported for this protein yet. Observed states export the representative PDB entry; inferred states need fitted models, not yet built.
States and transitions
No states defined.
| State | Basis | Parent | Description |
|---|
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-8Q84-1 RCSB PDB | cryoem | resolution: 3.15 Å | in vitro Outer kinetochore Dam1 protomer dimer Ndc80-Nuf2 coiled-coil complex · Kinetochore protein NDC80 (chains A,F) | unmapped |
| EV-PDB-8V11-1 RCSB PDB | xray | resolution: 3.95 Å | in vitro · -193.15 °C · pH 7.5 Structure of a Saccharomyces cerevisiae Ipl1 peptide Bound to dwarf Ndc80 complex · Kinetochore protein NDC80 (chains A,E) | unmapped |
| EV-PDB-8V10-1 RCSB PDB | xray | resolution: 3.02 Å | in vitro · -195.79 °C · pH 6.1 · ligands NI Structure of a Saccharomyces cerevisiae Mps1 peptide bound to dwarf Ndc80 Complex · Kinetochore protein NDC80 (chains A) | unmapped |
| EV-PDB-8QAU-3 RCSB PDB | cryoem | resolution: 3.54 Å | in vitro · ligands GDP, GTP, MG, TA1 Outer kinetochore Ndc80-Dam1 alpha/beta-tubulin complex · Kinetochore protein NDC80 (chains A) | unmapped |
| EV-PDB-8Q85-1 RCSB PDB | cryoem | resolution: 3.97 Å | in vitro Outer kinetochore Dam1 protomer monomer Ndc80-Nuf2 coiled-coil complex · Kinetochore protein NDC80 (chains F) | unmapped |
| EV-PDB-8G0Q-1 RCSB PDB | xray | resolution: 3.22 Å | in vitro · -173.15 °C Crystal structure of the yeast Ndc80:Nuf2 head region with a bound Dam1 segment · Kinetochore protein NDC80 (chains A,C) | unmapped |
| EV-PDB-7KDF-1 RCSB PDB | xray | resolution: 2.72 Å | in vitro · -193.15 °C · pH 7.3 · ligands SO4 Structure of Stu2 Bound to dwarf Ndc80c · NDC80 isoform 1,NDC80 isoform 1 (chains A) | unmapped |
| EV-PDB-5TD8-1 RCSB PDB | xray | resolution: 7.531 Å | in vitro · -173.15 °C · ligands HG Crystal structure of an Extended Dwarf Ndc80 Complex · Kinetochore protein NDC80 (chains A) | unmapped |
| EV-PDB-5TCS-1 RCSB PDB | xray | resolution: 2.8313 Å | in vitro · -173.15 °C · pH 6 · ligands MG Crystal structure of a Dwarf Ndc80 Tetramer · Kinetochore protein NDC80 (chains A) | unmapped |
| EV-BMRB-51814 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 6.5 Backbone Chemical Shift Assignments (1H, 13C, and 15N) for the CH Domain of Yeast Kinetochore Protein Ndc80 · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-AFDB-AF-P40460-F1 AlphaFold DB | af_prediction | plddt: 77 pLDDT | in silico AlphaFold DB prediction AF-P40460-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-691 | unmapped |
| EV-SMASH-SMFS-000156 SMASH-BIO | force_spectroscopy | study_record | in vitro Kinetochore-microtubule attachments are strengthened by Cnn1 stabilization of Stu2 · Kinetochore (Ndc80/Dam1); optical tweezers; experiment class: chromatin_mechanics, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-000535 SMASH-BIO | force_spectroscopy | study_record | in vitro Ndc80 complex, a conserved coupler for kinetochore-microtubule motility, is a sliding molecular clutch · Kinetochore (Ndc80/Dam1); force spectroscopy unspecified; experiment class: chromatin_mechanics, force_generation_motor, force_generation_polymerization, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-001224 SMASH-BIO | force_spectroscopy | study_record | in vitro Mechanical coupling coordinates microtubule growth · Kinetochore (Ndc80/Dam1); optical tweezers; experiment class: chromatin_mechanics, force_generation_polymerization, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001437 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 6.8 Catching the Conformational Wave: Measuring the Working Strokes of Protofilaments as They Curl Outward from Disassembling Microtubule Tips. · Kinetochore (Ndc80/Dam1); optical tweezers; experiment class: chromatin_mechanics, force_generation_motor | unmapped |
| EV-SMASH-SMFS-001736 SMASH-BIO | force_spectroscopy | study_record | in vitro Strain stiffening of Ndc80 complexes attached to microtubule plus ends · Kinetochore (Ndc80/Dam1); optical tweezers; experiment class: chromatin_mechanics, force_generation_motor, force_generation_polymerization | unmapped |
| EV-SMASH-SMFS-001737 SMASH-BIO | force_spectroscopy | study_record | in vitro Strain stiffening of Ndc80 complexes attached to microtubule plus ends. · Kinetochore (Ndc80/Dam1); optical tweezers; experiment class: chromatin_mechanics, force_generation_polymerization | unmapped |
| EV-SMASH-SMFS-001777 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 6.9 Ultrafast Force-Clamp Spectroscopy of Microtubule-Binding Proteins. · Kinetochore (Ndc80/Dam1); optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-002174 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 6.9 Tension promotes kinetochore-microtubule release by Aurora B kinase. · Kinetochore (Ndc80/Dam1); force spectroscopy unspecified; experiment class: chromatin_mechanics, force_generation_polymerization, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-003192 SMASH-BIO | force_spectroscopy | study_record | in vitro Molecular force spectroscopy of kinetochore-microtubule attachment in silico: Mechanical signatures of an unusual catch bond and collective effects. · Kinetochore (Ndc80/Dam1); force spectroscopy unspecified; experiment class: force_generation_polymerization, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-003236-r0 SMASH-BIO | force_spectroscopy | contour_length: 230 nm | in vitro Probing Mitotic CENP-E Kinesin with the Tethered Cargo Motion Assay and Laser Tweezers. · Kinetochore (Ndc80/Dam1); optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-003463 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 6.9 Direct measurement of conformational strain energy in protofilaments curling outward from disassembling microtubule tips. · Kinetochore (Ndc80/Dam1); optical tweezers; experiment class: chromatin_mechanics, force_generation_motor | unmapped |
| EV-SMASH-SMFS-003639 SMASH-BIO | force_spectroscopy | study_record | in vitro Phenomenological and microscopic theories for catch bonds. · Kinetochore (Ndc80/Dam1); afm smfs; experiment class: force_generation_motor, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-003833 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.6 CENP-A and H3 Nucleosomes Display a Similar Stability to Force-Mediated Disassembly. · Kinetochore (Ndc80/Dam1); magnetic tweezers; experiment class: chromatin_mechanics, nucleic_acid_protein_interaction, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-004528 SMASH-BIO | force_spectroscopy | study_record | in vitro Distribution of lifetimes of kinetochore-microtubule attachments: interplay of energy landscape, molecular motors and microtubule (de-)polymerization. · Kinetochore (Ndc80/Dam1); force spectroscopy unspecified; experiment class: chromatin_mechanics, force_generation_motor, force_generation_polymerization, receptor_ligand_adhesion, unfolding_protein | unmapped |
| EV-SMASH-SMFS-004902 SMASH-BIO | force_spectroscopy | study_record | in vitro Kinetochore kinesin CENP-E is a processive bi-directional tracker of dynamic microtubule tips. · Kinetochore (Ndc80/Dam1); optical tweezers; experiment class: chromatin_mechanics, force_generation_motor | unmapped |
| EV-SMASH-SMFS-004917 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 6.9 Measurements of forces produced by the mitotic spindle using optical tweezers. · Kinetochore (Ndc80/Dam1); optical tweezers; experiment class: chromatin_mechanics | unmapped |
| EV-SMASH-SMFS-004983 SMASH-BIO | force_spectroscopy | study_record | in vitro Phosphoregulation promotes release of kinetochores from dynamic microtubules via multiple mechanisms. · Kinetochore (Ndc80/Dam1); optical tweezers; experiment class: chromatin_mechanics, force_generation_polymerization, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-005768 SMASH-BIO | force_spectroscopy | study_record | in vitro Direct physical study of kinetochore-microtubule interactions by reconstitution and interrogation with an optical force clamp. · Kinetochore (Ndc80/Dam1); optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-006204 SMASH-BIO | force_spectroscopy | study_record | in vitro Spindle checkpoint regulated by nonequilibrium collective spindle-chromosome interaction; relationship to single DNA molecule force-extension formula. · Kinetochore (Ndc80/Dam1); force spectroscopy unspecified; experiment class: chromatin_mechanics | unmapped |
| EV-SMASH-SMFS-006917 SMASH-BIO | force_spectroscopy | study_record | in vitro The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement. · Kinetochore (Ndc80/Dam1); optical tweezers; experiment class: chromatin_mechanics, force_generation_polymerization | unmapped |
Conflicts
No conflicts found among comparable evidence.
31 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.