Replication protein A 70 kDa DNA-binding subunit
UniProt P27694 · gene RPA1 · Homo sapiens · 616 residues
88evidence items
0mapped to a state
0states
8techniques
6sources
0conflicts
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | xray | cryoem | nmr_structure | nmr_shifts | saxs_sans | force_spectroscopy | integrative | af_prediction | Total |
|---|---|---|---|---|---|---|---|---|---|---|
| No states defined yet. | ||||||||||
Unmapped evidence: 88 of 88 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 0 |
| BMRB | 1 | 0 |
| DisProt | 2 | 0 |
| RCSB PDB | 48 | 0 |
| SASBDB | 4 | 0 |
| SMASH-BIO | 32 | 0 |
Structure
No state models are exported for this protein yet. Observed states export the representative PDB entry; inferred states need fitted models, not yet built.
States and transitions
No states defined.
| State | Basis | Parent | Description |
|---|
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-1EWI-1 RCSB PDB | nmr_structure | structure | in vitro · 24.85 °C · pH 7.6 HUMAN REPLICATION PROTEIN A: GLOBAL FOLD OF THE N-TERMINAL RPA-70 DOMAIN REVEALS A BASIC CLEFT AND FLEXIBLE C-TERMINAL LINKER · REPLICATION PROTEIN A (chains A) | unmapped |
| EV-PDB-1JMC-2 RCSB PDB | xray | resolution: 2.4 Å | in vitro · -173.15 °C · pH 6.8 SINGLE STRANDED DNA-BINDING DOMAIN OF HUMAN REPLICATION PROTEIN A BOUND TO SINGLE STRANDED DNA, RPA70 SUBUNIT, RESIDUES 183-420 · PROTEIN (REPLICATION PROTEIN A (RPA)) (chains A) | unmapped |
| EV-PDB-1L1O-3 RCSB PDB | xray | resolution: 2.8 Å | in vitro · -173.15 °C · pH 7.8 · ligands ZN Structure of the human Replication Protein A (RPA) trimerization core · Replication protein A 70 kDa DNA-binding subunit (chains C,F) | unmapped |
| EV-PDB-2B3G-1 RCSB PDB | xray | resolution: 1.6 Å | in vitro · pH 7.9 p53N (fragment 33-60) bound to RPA70N · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4IJH-1 RCSB PDB | xray | resolution: 1.498 Å | in vitro · -173.15 °C · pH 6.5 · ligands 1EJ Fragment-based Discovery of Protein-Protein Interaction Inhibitors of Replication Protein A · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4IJL-1 RCSB PDB | xray | resolution: 1.7 Å | in vitro · -173.15 °C · pH 6.5 · ligands 1EK Fragment-based Discovery of Protein-Protein Interaction Inhibitors of Replication Protein A · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4IPC-1 RCSB PDB | xray | resolution: 1.22 Å | in vitro · -173.15 °C · pH 6.5 Structure of the N-terminal domain of RPA70, E7R mutant · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4IPD-1 RCSB PDB | xray | resolution: 1.51 Å | in vitro · -173.15 °C · pH 5.5 Structure of the N-terminal domain of RPA70, E100R mutant · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4IPG-1 RCSB PDB | xray | resolution: 1.58 Å | in vitro · -173.15 °C · pH 5 Structure of the N-terminal domain of RPA70, E7R, E100R mutant · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4IPH-1 RCSB PDB | xray | resolution: 1.94 Å | in vitro · -173.15 °C · pH 6.5 · ligands 1FJ Structure of N-terminal domain of RPA70 in complex with VU079104 inhibitor · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4LUO-1 RCSB PDB | xray | resolution: 1.54 Å | in vitro · -173.15 °C · pH 6.5 · ligands 1DZ Fragment-Based Discovery of a Potent Inhibitor of Replication Protein A Protein-Protein Interactions · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4LUV-1 RCSB PDB | xray | resolution: 1.4 Å | in vitro · -173.15 °C · pH 6.5 · ligands 1DZ, 1XS Fragment-Based Discovery of a Potent Inhibitor of Replication Protein A Protein-Protein Interactions · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4LW1-1 RCSB PDB | xray | resolution: 1.631 Å | in vitro · -173.15 °C · pH 6.5 · ligands 1XS Fragment-Based Discovery of a Potent Inhibitor of Replication Protein A Protein-Protein Interactions · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4LWC-1 RCSB PDB | xray | resolution: 1.61 Å | in vitro · -173.15 °C · pH 6.5 · ligands 1XU Fragment-Based Discovery of a Potent Inhibitor of Replication Protein A Protein-Protein Interactions · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4O0A-1 RCSB PDB | xray | resolution: 1.2 Å | in vitro · -173.15 °C · pH 6.5 · ligands 2P9 Fragment-Based Discovery of a Potent Inhibitor of Replication Protein A Protein-Protein Interactions · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4R4C-1 RCSB PDB | xray | resolution: 1.4 Å | in vitro · -173.15 °C · pH 6.5 · ligands 3HS Structure of RPA70N in complex with 5-(4-((4-(5-carboxyfuran-2-yl)-2-chlorobenzamido)methyl)phenyl)-1-(3,4-dichlorophenyl)-1H-pyrazole-3-carboxylic acid · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4R4I-1 RCSB PDB | xray | resolution: 1.4 Å | in vitro · -173.15 °C · pH 6.5 · ligands 3HV Structure of RPA70N in complex with 5-(4-((6-(5-carboxyfuran-2-yl)-1-thioxo-3,4-dihydroisoquinolin-2(1H)-yl)methyl)phenyl)-1-(3,4-dichlorophenyl)-1H-pyrazole-3-carboxylic acid · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4R4O-1 RCSB PDB | xray | resolution: 1.33 Å | in vitro · -173.15 °C · pH 6.5 · ligands 3HW Crystal structure of RPA70N in complex with 5-(4-((4-(5-carboxyfuran-2-yl)benzyl)carbamothioyl)phenyl)-1-(3,4-dichlorophenyl)-1H-pyrazole-3-carboxylic acid · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4R4T-1 RCSB PDB | xray | resolution: 1.28 Å | in vitro · -173.15 °C · pH 6.5 · ligands 3J0 Crystal Structure of RPA70N in complex with 5-(4-((4-(5-carboxyfuran-2-yl)phenylthioamido)methyl)phenyl)-1-(3,4-dichlorophenyl)-1H-pyrazole-3-carboxylic acid · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-5E7N-1 RCSB PDB | xray | resolution: 1.21 Å | in vitro · -173.15 °C · pH 6.5 · ligands 5KR Crystal Structure of RPA70N in complex with VU0085636 · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-5EAY-1 RCSB PDB | xray | resolution: 1.55 Å | in vitro · -173.15 °C · pH 8 Crystal structure of a Dna2 peptide in complex with Rpa 70N · Replication protein A 70 kDa DNA-binding subunit (chains A,B,C,D) | unmapped |
| EV-PDB-5N85-1 RCSB PDB | xray | resolution: 2 Å | in vitro · -173.15 °C · pH 4.5 Structure of RPA70N in complex with PrimPol (fragment 514-525) · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-5N8A-2 RCSB PDB | xray | resolution: 1.28 Å | in vitro · -173.15 °C · pH 6 Structure of RPA70N in complex with PrimPol (fragment 480-560) · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-7XUT-1 RCSB PDB | xray | resolution: 1.6 Å | in vitro · -173.15 °C Crystal structure of RPA70N-WRN fusion · fusion protein of Replication protein A 70 kDa DNA-binding subunit and Werner syndrome ATP-dependent helicase (chains A,B) | unmapped |
| EV-PDB-7XUV-1 RCSB PDB | xray | resolution: 1.6 Å | in vitro · -173.15 °C · pH 4.6 Crystal structure of RPA70N-RMI1 fusion · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-7XUW-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · -173.15 °C · pH 4.6 Crystal structure of RPA70N-BLMp2 fusion · fusion protein of Replication protein A 70 kDa DNA-binding subunit and Bloom syndrome protein (chains A) | unmapped |
| EV-PDB-7XV0-1 RCSB PDB | xray | resolution: 1.5 Å | in vitro · -173.15 °C · pH 5.6 Crystal structure of RPA70N-BLMp1 fusion · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-7XV1-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · -173.15 °C Crystal structure of RPA70N-HelB fusion · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-7XV4-1 RCSB PDB | xray | resolution: 1.6 Å | in vitro · -173.15 °C · pH 8.5 Crystal structure of RPA70N-ATRIP fusion · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-8JZV-1 RCSB PDB | xray | resolution: 1.5 Å | in vitro · -173.15 °C RPA70N-ETAA1 fusion · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-8JZY-1 RCSB PDB | xray | resolution: 1.5 Å | in vitro · -173.15 °C RPA70N-RAD9 fusion · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-8RK2-1 RCSB PDB | cryoem | resolution: 3.2 Å | in vitro Human Replication protein A (RPA; trimeric core) - ssDNA complex · Replication protein A 70 kDa DNA-binding subunit, N-terminally processed (chains A) | unmapped |
| EV-PDB-9A03-1 RCSB PDB | integrative | structure | in vitro Integrative model of 3' Nucleotide excision repair complex of XPA-DBD and RPA70AB · Subunit A (chains A) | unmapped |
| EV-PDB-9A1V-1 RCSB PDB | integrative | structure | in vitro · ligands ZN Integrative model of Nucleotide excision repair complex of XPA and RPA on 5' junction substrate · RPA70 (chains A) | unmapped |
| EV-PDB-9A1Y-1 RCSB PDB | integrative | structure | in vitro · ligands ZN Integrative model of Nucleotide excision repair complex of XPA and RPA on 3' junction substrate · RPA70 (chains A) | unmapped |
| EV-PDB-9A88-12 RCSB PDB | integrative | structure | in vitro · ligands MG, SF4, ZN Structure of the pre-incision complex in nucleotide excision repair · Replication protein A 70 kDa DNA-binding subunit, N-terminally processed (chains L) | unmapped |
| EV-PDB-9J1S-1 RCSB PDB | xray | resolution: 1.55 Å | in vitro · -173.15 °C · pH 7 Human replication protein A: RPA70 subunit N-terminal domain · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-9MJ5-3 RCSB PDB | cryoem | resolution: 3.5 Å | in vitro · ligands DCP, MG, ZN Catalytic domain of human DNA polymerase alpha in complex with DNA and RPA · Replication protein A 70 kDa DNA-binding subunit (chains C) | unmapped |
| EV-PDB-9PD3-12 RCSB PDB | cryoem | resolution: 3.3 Å | in vitro · ligands SF4, ZN NER dual incision complex - DuIS · Replication protein A 70 kDa DNA-binding subunit (chains N) | unmapped |
| EV-PDB-9PD4-11 RCSB PDB | cryoem | resolution: 3.4 Å | in vitro · ligands SF4, ZN NER dual incision complex - DuIM · Replication protein A 70 kDa DNA-binding subunit (chains N) | unmapped |
| EV-PDB-9A89-12 RCSB PDB | integrative | structure | in vitro · ligands MG, SF4, ZN Structure of the pre-incision complex in nucleotide excision repair with tilted XPG core · Replication protein A 70 kDa DNA-binding subunit, N-terminally processed (chains L) | unmapped |
| EV-PDB-9A04-1 RCSB PDB | integrative | structure | in vitro Integrative model of 5' Nucleotide excision repair complex of XPA-DBD and RPA70AB · Subunit A (chains A) | unmapped |
| EV-PDB-8K00-1 RCSB PDB | xray | resolution: 1.4 Å | in vitro · -173.15 °C RPA70N-MRE11 fusion · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4R4Q-1 RCSB PDB | xray | resolution: 1.35 Å | in vitro · -173.15 °C · pH 6.5 · ligands 3HZ Crystal structure of RPA70N in complex with 5-(3-((N-(4-(5-carboxyfuran-2-yl)benzyl)acetamido)methyl)phenyl)-1-(3,4-dichlorophenyl)-1H-pyrazole-3-carboxylic acid · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-4NB3-1 RCSB PDB | xray | resolution: 1.35 Å | in vitro · -173.15 °C · pH 6.5 Crystal structure of RPA70N in complex with a 3,4 dichlorophenylalanine ATRIP derived peptide · Replication protein A 70 kDa DNA-binding subunit (chains A,B) | unmapped |
| EV-PDB-4LUZ-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · -173.15 °C · pH 6.5 · ligands 1XT Fragment-Based Discovery of a Potent Inhibitor of Replication Protein A Protein-Protein Interactions · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-2B29-1 RCSB PDB | xray | resolution: 1.6 Å | in vitro · -173.15 °C · pH 8.5 N-terminal domain of the RPA70 subunit of human replication protein A. · Replication protein A 70 kDa DNA-binding subunit (chains A) | unmapped |
| EV-PDB-1FGU-1 RCSB PDB | xray | resolution: 2.5 Å | in vitro · -173.15 °C · pH 8.5 SSDNA-BINDING DOMAIN OF THE LARGE SUBUNIT OF REPLICATION PROTEIN A · REPLICATION PROTEIN A 70 KDA DNA-BINDING SUBUNIT (chains A,B) | unmapped |
| EV-BMRB-5822 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 7.2 1H, 15N and 13C assignments of single-stranded DNA binding domains from the 70 kDa subunit of Human Replication Protein A · BMRB data: 1H chemical shifts, 13C chemical shifts, 15N chemical shifts | unmapped |
| EV-SASBDB-SASDH44 SASBDB | saxs_sans | radius_of_gyration: 3.138 nm ± 0.05 | in vitro · 20 °C · pH 7.5 · ligands partner: 3-prime Nucleotide Excision Repair Junction Model Substrate, partner: DNA repair protein complementing XP-A cells A key interaction with RPA orients XPA in NER complexes. · Replication protein A 70 kDa DNA-binding subunit with DNA repair protein complementing XP-A cells, 3-prime Nucleotide Excision Repair Junction Model Substrate | unmapped |
| EV-SASBDB-SASDH54 SASBDB | saxs_sans | radius_of_gyration: 2.947 nm ± 0.01 | in vitro · pH 7.5 · ligands partner: 5-prime Nucleotide Excision Repair Junction Model Substrate, partner: DNA repair protein complementing XP-A cells A key interaction with RPA orients XPA in NER complexes. · Replication protein A 70 kDa DNA-binding subunit with DNA repair protein complementing XP-A cells, 5-prime Nucleotide Excision Repair Junction Model Substrate | unmapped |
| EV-SASBDB-SASDP24 SASBDB | saxs_sans | radius_of_gyration: 4.626 nm ± 0.932 | in vitro · 20 °C · pH 8 · ligands partner: 5-prime ss-ds DNA junction NER model substrate, partner: DNA repair protein complementing XP-A cells, partner: Replication protein A 14 kDa subunit, partner: Replication protein A 32 kDa subunit Two interaction surfaces between XPA and RPA organize the preincision complex in nucleotide excision repair · Replication protein A 70 kDa DNA-binding subunit with Replication protein A 14 kDa subunit, DNA repair protein complementing XP-A cells, Replication protein A 32 kDa subunit, 5-prime ss-ds DNA junction NER model substrate | unmapped |
| EV-SASBDB-SASDPZ3 SASBDB | saxs_sans | radius_of_gyration: 4.286 nm ± 0.512 | in vitro · 20 °C · pH 8 · ligands partner: 3-prime ss-ds DNA junction NER model substrate, partner: DNA repair protein complementing XP-A cells, partner: Replication protein A 14 kDa subunit, partner: Replication protein A 32 kDa subunit Two interaction surfaces between XPA and RPA organize the preincision complex in nucleotide excision repair · Replication protein A 70 kDa DNA-binding subunit with DNA repair protein complementing XP-A cells, Replication protein A 32 kDa subunit, Replication protein A 14 kDa subunit, 3-prime ss-ds DNA junction NER model substrate | unmapped |
| EV-DISPROT-DP00061r005 DisProt | nmr_shifts | disorder_region: 60 residues | in vitro DisProt DP00061: disorder, residues 109-168 · residues 109-168; evidence: nuclear magnetic resonance spectroscopy evidence used in manual assertion | unmapped |
| EV-DISPROT-DP00061r007 DisProt | nmr_shifts | disorder_region: 76 residues | in vitro DisProt DP00061: disorder, residues 105-180 · residues 105-180; evidence: nuclear magnetic resonance spectroscopy evidence used in manual assertion | unmapped |
| EV-AFDB-AF-P27694-F1 AlphaFold DB | af_prediction | plddt: 83.81 pLDDT | in silico AlphaFold DB prediction AF-P27694-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-616 | unmapped |
| EV-SMASH-SMFS-000010-r0 SMASH-BIO | force_spectroscopy | velocity: 1100 bp/s | in vitro A superfamily 1 helicase translocates on double-stranded DNA · Replication protein A; optical tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-000083 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Dpb11 facilitates the colocalization of Mec1-Ddc2 with its activators on gapped DNA. · Replication protein A; afm smfs; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-000214 SMASH-BIO | force_spectroscopy | study_record | in vitro Negative supercoiling facilitates human TOP3α-mediated decatenation of DNA braids via catenation junction melting · Replication protein A; optical tweezers; experiment class: chromatin_mechanics, nucleic_acid_mechanics, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-000247 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 RPA directly stimulates Mer3 helicase processivity to ensure normal crossover formation in meiosis. · Replication protein A; magnetic tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-000427 SMASH-BIO | force_spectroscopy | study_record | in vitro Dpb11 facilitates the colocalization of Mec1-Ddc2 with its activators on gapped DNA · Replication protein A; afm smfs; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-000539 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 New feature of hMEIOB and hSPATA22 binding to ssDNA from a single-molecule perspective. · Replication protein A; magnetic tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-000581 SMASH-BIO | force_spectroscopy | study_record | in vitro RPA directly stimulates Mer3/HFM1 helicase processivity to ensure normal crossover formation in meiosis · Replication protein A; magnetic tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-000610 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Single-Molecule Visualization of BLM-DNA2-Mediated DNA End Resection Using DNA Curtains. · Replication protein A; hydrodynamic stretching; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-000778 SMASH-BIO | force_spectroscopy | study_record | in vitro Force-fluorescence setup for observing protein-DNA interactions under load. · Replication protein A; magnetic tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication, unfolding_nucleic_acid, unfolding_protein | unmapped |
| EV-SMASH-SMFS-000917 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 8 Rapid Long-distance Migration of RPA on Single Stranded DNA Occurs Through Intersegmental Transfer Utilizing Multivalent Interactions. · Replication protein A; optical tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-000984 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 The human Shu complex promotes RAD51 activity by modulating RPA dynamics on ssDNA · Replication protein A; optical tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-000985 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 The human Shu complex promotes RAD51 activity by modulating RPA dynamics on ssDNA. · Replication protein A; optical tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001026 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 8.1 "Helicase" Activity promoted through dynamic interactions between a ssDNA translocase and a diffusing SSB protein. · Replication protein A; optical tweezers; experiment class: force_generation_motor, nucleic_acid_mechanics, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001291 SMASH-BIO | force_spectroscopy | study_record | in vitro Rapid long-distance migration of RPA on single stranded DNA occurs through intersegmental transfer utilizing multivalent interactions · Replication protein A; optical tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001327 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Single-molecule visualization of Pif1 helicase translocation on single-stranded DNA. · Replication protein A; hydrodynamic stretching; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001366 SMASH-BIO | force_spectroscopy | study_record | in vitro Understanding G-Quadruplex Biology and Stability Using Single-Molecule Techniques. · Replication protein A; optical tweezers; experiment class: nucleic_acid_protein_interaction, receptor_ligand_adhesion, translation_transcription_replication, unfolding_nucleic_acid, unfolding_protein | unmapped |
| EV-SMASH-SMFS-001707 SMASH-BIO | force_spectroscopy | study_record | in vitro Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding. · Replication protein A; optical tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001731 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Single-stranded nucleic acid binding and coacervation by linker histone H1. · Replication protein A; optical tweezers; experiment class: chromatin_mechanics, nucleic_acid_mechanics, nucleic_acid_protein_interaction, receptor_ligand_adhesion, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001793 SMASH-BIO | force_spectroscopy | study_record | in vitro “Helicase” Activity Promoted Through Dynamic Interactions Between a ssDNA Translocase and a Diffusing SSB Protein · Replication protein A; optical tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001943 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis. · Replication protein A; optical tweezers; experiment class: nucleic_acid_mechanics, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-002172 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.6 Switch-like control of helicase processivity by single-stranded DNA binding protein. · Replication protein A; optical tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-002698 SMASH-BIO | force_spectroscopy | study_record | in vitro Dynamic interactions of the homologous pairing 2 (Hop2)-meiotic nuclear divisions 1 (Mnd1) protein complex with meiotic presynaptic filaments in budding yeast. · Replication protein A; hydrodynamic stretching; experiment class: nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-002903 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Single-molecule visualization of human BLM helicase as it acts upon double- and single-stranded DNA substrates. · Replication protein A; hydrodynamic stretching; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-003002 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 Assessing Protein Dynamics on Low-Complexity Single-Stranded DNA Curtains. · Replication protein A; hydrodynamic stretching; experiment class: membrane_mechanics, nucleic_acid_protein_interaction, receptor_ligand_adhesion, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-003003 SMASH-BIO | force_spectroscopy | study_record | in vitro Assessing protein dynamics on low complexity single-strand DNA curtains · Replication protein A; hydrodynamic stretching; experiment class: membrane_mechanics, nucleic_acid_protein_interaction, receptor_ligand_adhesion, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-003099 SMASH-BIO | force_spectroscopy | study_record | in vitro Facilitated Unbinding via Multivalency-Enabled Ternary Complexes: New Paradigm for Protein-DNA Interactions. · Replication protein A; magnetic tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, receptor_ligand_adhesion, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-003201 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 8 Multiple RPAs make WRN syndrome protein a superhelicase. · Replication protein A; magnetic tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-003408 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Annealing helicase HARP closes RPA-stabilized DNA bubbles non-processively. · Replication protein A; optical tweezers; experiment class: force_generation_motor, nucleic_acid_mechanics, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-004211 SMASH-BIO | force_spectroscopy | study_record | in vitro Experimental phase diagram of negatively supercoiled DNA measured by magnetic tweezers and fluorescence. · Replication protein A; optical tweezers; experiment class: nucleic_acid_mechanics, nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-004500 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 Concentration-dependent exchange of replication protein A on single-stranded DNA revealed by single-molecule imaging. · Replication protein A; hydrodynamic stretching; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-005053 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Substrate-selective repair and restart of replication forks by DNA translocases. · Replication protein A; magnetic tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-005959 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Torsional regulation of hRPA-induced unwinding of double-stranded DNA. · Replication protein A; magnetic tweezers; experiment class: nucleic_acid_mechanics, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
Conflicts
No conflicts found among comparable evidence.
84 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.