Single-stranded DNA-binding protein
UniProt P0AGE0 · gene ssb · Escherichia coli (strain K12) · 178 residues
38evidence items
0mapped to a state
0states
4techniques
5sources
0conflicts
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | xray | nmr_shifts | force_spectroscopy | af_prediction | Total |
|---|---|---|---|---|---|---|
| No states defined yet. | ||||||
Unmapped evidence: 38 of 38 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 0 |
| BMRB | 1 | 0 |
| DisProt | 1 | 0 |
| RCSB PDB | 14 | 0 |
| SMASH-BIO | 21 | 0 |
Structure
No state models are exported for this protein yet. Observed states export the representative PDB entry; inferred states need fitted models, not yet built.
States and transitions
No states defined.
| State | Basis | Parent | Description |
|---|
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-1EYG-2 RCSB PDB | xray | resolution: 2.8 Å | in vitro · -170.15 °C · pH 7.5 Crystal structure of chymotryptic fragment of E. coli ssb bound to two 35-mer single strand DNAS · SINGLE-STRAND DNA-BINDING PROTEIN (chains A,B,C,D) | unmapped |
| EV-PDB-1KAW-1 RCSB PDB | xray | resolution: 2.9 Å | in vitro · -173.15 °C STRUCTURE OF SINGLE STRANDED DNA BINDING PROTEIN (SSB) · SINGLE-STRANDED DNA BINDING PROTEIN (chains A,B,C,D) | unmapped |
| EV-PDB-1QVC-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · 14.85 °C · pH 9 CRYSTAL STRUCTURE ANALYSIS OF SINGLE STRANDED DNA BINDING PROTEIN (SSB) FROM E.COLI · SINGLE STRANDED DNA BINDING PROTEIN MONOMER (chains A,B,C,D) | unmapped |
| EV-PDB-1SRU-1 RCSB PDB | xray | resolution: 3.3 Å | in vitro · -173.15 °C · pH 7.5 Crystal structure of full length E. coli SSB protein · Single-strand binding protein (chains A,B,C,D) | unmapped |
| EV-PDB-3C94-2 RCSB PDB | xray | resolution: 2.7 Å | in vitro · -163.15 °C · pH 8 · ligands MG ExoI/SSB-Ct complex · Single-stranded DNA-binding C-terminal tail peptide (chains B,C) | unmapped |
| EV-PDB-3Q8D-2 RCSB PDB | xray | resolution: 2.3 Å | in vitro · -173.15 °C · pH 6.5 · ligands CPS, GOL, MLT E. coli RecO complex with SSB C-terminus · Single-stranded DNA-binding protein (chains E,F) | unmapped |
| EV-PDB-3UF7-2 RCSB PDB | xray | resolution: 1.2 Å | in vitro · -163.15 °C · pH 6.5 · ligands SO4 Co-crystal structure of Escherichia coli uracil-DNA glycosylase and a C-terminal fragement of the single-stranded DNA-binding protein · Single-stranded DNA-binding protein (chains B,C) | unmapped |
| EV-PDB-4Z0U-2 RCSB PDB | xray | resolution: 2 Å | in vitro · -173.15 °C · pH 7 RNase HI/SSB-Ct complex · SSB-Ct Peptide (chains D,E) | unmapped |
| EV-PDB-8TG7-2 RCSB PDB | xray | resolution: 1.775 Å | in vitro · -173.15 °C · pH 8.6 Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 2 · Plasmid-derived single-stranded DNA-binding protein (chains C,D) | unmapped |
| EV-PDB-8TG8-2 RCSB PDB | xray | resolution: 1.576 Å | in vitro · -173.15 °C · pH 8.6 Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 3 · TRP-MET-ASP-PHE-ASP-ASP-ASP-ILE-PRO-PHE (chains B) | unmapped |
| EV-PDB-8TGC-2 RCSB PDB | xray | resolution: 1.484 Å | in vitro · -173.15 °C · pH 8.5 Structure of Red beta C-terminal domain in complex with SSB C-terminal peptide, Form 4 · Plasmid-derived single-stranded DNA-binding protein (chains C,D) | unmapped |
| EV-PDB-4MZ9-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro Revised structure of E. coli SSB · Single-stranded DNA-binding protein (chains A,B,C,D) | unmapped |
| EV-PDB-3SXU-3 RCSB PDB | xray | resolution: 1.85 Å | in vitro · -173.15 °C · pH 4.6 Structure of the E. coli SSB-DNA polymerase III interface · SSB peptide (chains C) | unmapped |
| EV-PDB-1EQQ-2 RCSB PDB | xray | resolution: 3.2 Å | in vitro · -173.15 °C · pH 9 SINGLE STRANDED DNA BINDING PROTEIN AND SSDNA COMPLEX · SINGLE STRANDED DNA BINDING PROTEIN (chains A,B,C,D) | unmapped |
| EV-BMRB-19446 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 3.4 Single-stranded DNA binding protein from E. coli (SSB) · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-DISPROT-DP02137r001 DisProt | xray | disorder_region: 65 residues | in vitro DisProt DP02137: disorder, residues 114-178 · residues 114-178; evidence: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion | unmapped |
| EV-AFDB-AF-P0AGE0-F1 AlphaFold DB | af_prediction | plddt: 80.75 pLDDT | in silico AlphaFold DB prediction AF-P0AGE0-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-178 | unmapped |
| EV-SMASH-SMFS-000010-r0 SMASH-BIO | force_spectroscopy | velocity: 1100 bp/s | in vitro A superfamily 1 helicase translocates on double-stranded DNA · SSB (E. coli); optical tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-000414 SMASH-BIO | force_spectroscopy | study_record | in vitro DNA polymerase actively and sequentially displaces single-stranded DNA-binding proteins · SSB (E. coli); afm smfs; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001041 SMASH-BIO | force_spectroscopy | study_record | in vitro A versatile and high-throughput flow-cell system combined with fluorescence imaging for simultaneous single-molecule force measurement and visualization. · SSB (E. coli); hydrodynamic stretching; experiment class: force_generation_motor, nucleic_acid_mechanics, nucleic_acid_protein_interaction, translation_transcription_replication, unfolding_protein | unmapped |
| EV-SMASH-SMFS-001371 SMASH-BIO | force_spectroscopy | study_record | in vitro Unravelling How Single-Stranded DNA Binding Protein Coordinates DNA Metabolism Using Single-Molecule Approaches. · SSB (E. coli); optical tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001707 SMASH-BIO | force_spectroscopy | study_record | in vitro Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding. · SSB (E. coli); optical tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-001988 SMASH-BIO | force_spectroscopy | study_record | in vitro Magnetic Tweezers-Based Single-Molecule Assays to Study Interaction of E. coli SSB with DNA and RecQ Helicase. · SSB (E. coli); magnetic tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-002143 SMASH-BIO | force_spectroscopy | study_record | in vitro Single-molecule insight into stalled replication fork rescue in Escherichia coli. · SSB (E. coli); optical tweezers; experiment class: translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-002172 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.6 Switch-like control of helicase processivity by single-stranded DNA binding protein. · SSB (E. coli); optical tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-002557 SMASH-BIO | force_spectroscopy | study_record | in vitro Switch-like control of helicase processivity by single-stranded DNA binding protein · SSB (E. coli); optical tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-003002 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 Assessing Protein Dynamics on Low-Complexity Single-Stranded DNA Curtains. · SSB (E. coli); hydrodynamic stretching; experiment class: membrane_mechanics, nucleic_acid_protein_interaction, receptor_ligand_adhesion, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-003003 SMASH-BIO | force_spectroscopy | study_record | in vitro Assessing protein dynamics on low complexity single-strand DNA curtains · SSB (E. coli); hydrodynamic stretching; experiment class: membrane_mechanics, nucleic_acid_protein_interaction, receptor_ligand_adhesion, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-003671 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 RecQ helicase triggers a binding mode change in the SSB-DNA complex to efficiently initiate DNA unwinding. · SSB (E. coli); optical tweezers; experiment class: nucleic_acid_mechanics, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-003855-r0 SMASH-BIO | force_spectroscopy | loading_rate: 60 pN/s | in vitro Defining Single Molecular Forces Required for Notch Activation Using Nano Yoyo. · SSB (E. coli); optical tweezers; experiment class: nucleic_acid_protein_interaction, receptor_ligand_adhesion, rheology_cell | unmapped |
| EV-SMASH-SMFS-004185 SMASH-BIO | force_spectroscopy | study_record | in vitro Disturbance-free rapid solution exchange for magnetic tweezers single-molecule studies. · SSB (E. coli); magnetic tweezers; experiment class: force_generation_polymerization, nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-004242 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 8 Imaging and energetics of single SSB-ssDNA molecules reveal intramolecular condensation and insight into RecOR function. · SSB (E. coli); magnetic tweezers; experiment class: nucleic_acid_protein_interaction, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-004500 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 Concentration-dependent exchange of replication protein A on single-stranded DNA revealed by single-molecule imaging. · SSB (E. coli); hydrodynamic stretching; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-004752 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 8.3 Ultrafast redistribution of E. coli SSB along long single-stranded DNA via intersegment transfer. · SSB (E. coli); optical tweezers; experiment class: nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-005053 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Substrate-selective repair and restart of replication forks by DNA translocases. · SSB (E. coli); magnetic tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-005327 SMASH-BIO | force_spectroscopy | study_record | in vitro Single-molecule analysis of SSB dynamics on single-stranded DNA. · SSB (E. coli); force spectroscopy unspecified; experiment class: nucleic_acid_mechanics, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-005484-r0 SMASH-BIO | force_spectroscopy | unbinding_force: 10 pN | in vitro EMSA and single-molecule force spectroscopy study of interactions between Bacillus subtilis single-stranded DNA-binding protein and single-stranded DNA. · SSB (E. coli); afm smfs; experiment class: nucleic_acid_protein_interaction, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-005952 SMASH-BIO | force_spectroscopy | study_record | in vitro The nature of the force-induced conformation transition of dsDNA studied by using single molecule force spectroscopy. · SSB (E. coli); afm smfs; experiment class: nucleic_acid_mechanics, nucleic_acid_protein_interaction | unmapped |
Conflicts
No conflicts found among comparable evidence.
38 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.