Chemotaxis protein CheY
UniProt P0AE67 · gene cheY · Escherichia coli (strain K12) · 129 residues
69evidence items
61mapped to a state
2states
5techniques
4sources
0conflicts
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | xray | nmr_structure | nmr_shifts | af_prediction | md | Total |
|---|---|---|---|---|---|---|---|
| inactive (unphosphorylated) CheY-inactive | observed | proc 38 | proc 4 | · | · | · | 42 |
| active (BeF3- phosphoryl mimic) CheY-active | observed (one method) | proc 19 | · | · | · | · | 19 |
Unmapped evidence: 8 of 69 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 0 |
| BMRB | 6 | 0 |
| MDDB | 1 | 0 |
| RCSB PDB | 61 | 61 |
Curated rules applied
chey-bef3-active: 18 itemschey-unphosphorylated: 41 items
Structure
Choose a state. Each model is badged observed or inferred; assumptions are one click away.
States and transitions
| State | Basis | Parent | Description |
|---|---|---|---|
| inactive (unphosphorylated) CheY-inactive | observed | — | Unphosphorylated receiver domain, as in 3CHY. |
| active (BeF3- phosphoryl mimic) CheY-active | observed | — | Activated conformation with BeF3- bound as a phosphoryl analogue, as in 1FQW. |
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-1AB6-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · 19.85 °C · pH 7.2 STRUCTURE OF CHEY MUTANT F14N, V86T · CHEMOTAXIS PROTEIN CHEY (chains A,B) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1C4W-1 RCSB PDB | xray | resolution: 1.84 Å | in vitro · -173.15 °C · pH 6.9 1.9 A STRUCTURE OF A-THIOPHOSPHONATE MODIFIED CHEY D57C · CHEMOTAXIS PROTEIN CHEY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1CEY-1 RCSB PDB | nmr_structure | structure | in vitro ASSIGNMENTS, SECONDARY STRUCTURE, GLOBAL FOLD, AND DYNAMICS OF CHEMOTAXIS Y PROTEIN USING THREE-AND FOUR-DIMENSIONAL HETERONUCLEAR (13C,15N) NMR SPECTROSCOPY · CHEY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1CHN-1 RCSB PDB | xray | resolution: 1.76 Å | in vitro · ligands MG MAGNESIUM BINDING TO THE BACTERIAL CHEMOTAXIS PROTEIN CHEY RESULTS IN LARGE CONFORMATIONAL CHANGES INVOLVING ITS FUNCTIONAL SURFACE · CHEY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1D4Z-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · -173.15 °C · pH 4.8 · ligands SO4 CRYSTAL STRUCTURE OF CHEY-95IV, A HYPERACTIVE CHEY MUTANT · CHEMOTAXIS PROTEIN CHEY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1E6K-1 RCSB PDB | xray | resolution: 2 Å | in vitro · 13.85 °C · pH 7 Two-component signal transduction system D12A mutant of CheY · Chemotaxis protein CheY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1EHC-1 RCSB PDB | xray | resolution: 2.26 Å | in vitro · 16.85 °C · pH 8.3 · ligands SO4 STRUCTURE OF SIGNAL TRANSDUCTION PROTEIN CHEY · CHEY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1F4V-1 RCSB PDB | xray | resolution: 2.22 Å | in vitro · -173.15 °C · pH 8.4 · ligands BEF, GOL, MG CRYSTAL STRUCTURE OF ACTIVATED CHEY BOUND TO THE N-TERMINUS OF FLIM · CHEMOTAXIS CHEY PROTEIN (chains A,B,C) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-1FFG-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · 24.85 °C · pH 5.5 · ligands MN CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY AT 2.1 A RESOLUTION · CHEMOTAXIS PROTEIN CHEY (chains A,C) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1FFS-1 RCSB PDB | xray | resolution: 2.4 Å | in vitro · 24.85 °C · pH 5.5 · ligands MN CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM CRYSTALS SOAKED IN ACETYL PHOSPHATE · CHEMOTAXIS PROTEIN CHEY (chains A,C) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1FFW-1 RCSB PDB | xray | resolution: 2.7 Å | in vitro · 24.85 °C · pH 5.5 · ligands MN, PON CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY WITH A BOUND IMIDO DIPHOSPHATE · CHEMOTAXIS PROTEIN CHEY (chains A,C) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1FQW-1 RCSB PDB | xray | resolution: 2.37 Å | in vitro · -173.15 °C · pH 7 · ligands BEF, MN CRYSTAL STRUCTURE OF ACTIVATED CHEY · CHEMOTAXIS CHEY PROTEIN (chains A,B) | active (BeF3- phosphoryl mimic) · curator:draft (unreviewed) · high Title: activated CheY; BeF3- (BEF) bound. |
| EV-PDB-1JBE-1 RCSB PDB | xray | resolution: 1.08 Å | in vitro · -173.15 °C · pH 8.3 · ligands GOL, SO4 1.08 A Structure of apo-Chey reveals meta-active conformation · Chemotaxis protein CheY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1KMI-1 RCSB PDB | xray | resolution: 2.9 Å | in vitro · -73.15 °C · pH 8.5 · ligands BCN, BEF, MG CRYSTAL STRUCTURE OF AN E.COLI CHEMOTAXIS PROTEIN, CHEZ · Chemotaxis protein cheY (chains Y) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-1UDR-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro CHEY MUTANT WITH LYS 91 REPLACED BY ASP, LYS 92 REPLACED BY ALA, ILE 96 REPLACED BY LYS AND ALA 98 REPLACED BY LEU (STABILIZING MUTATIONS IN HELIX 4) · CHEY PROTEIN (chains A,B,C,D) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1VLZ-1 RCSB PDB | xray | resolution: 2.05 Å | in vitro UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY · CHEY (chains A,B) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1YMU-1 RCSB PDB | xray | resolution: 2.3 Å | in vitro · 19.85 °C · pH 7.2 SIGNAL TRANSDUCTION PROTEIN CHEY MUTANT WITH MET 17 REPLACED BY GLY (M17G) · CHEY (chains A,B) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1YMV-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · 3.85 °C · pH 6.8 · ligands MG SIGNAL TRANSDUCTION PROTEIN CHEY MUTANT WITH PHE 14 REPLACED BY GLY, SER 15 REPLACED BY GLY, AND MET 17 REPLACED BY GLY · CHEY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-2B1J-1 RCSB PDB | xray | resolution: 2.4 Å | in vitro · 24.85 °C · pH 6 · ligands MG Crystal Structure of Unphosphorylated CheY Bound to the N-Terminus of FliM · Chemotaxis protein cheY (chains A,B) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-2ID9-1 RCSB PDB | xray | resolution: 1.75 Å | in vitro · -173.15 °C · pH 5.84 1.85 A Structure of T87I/Y106W Phosphono-CheY · Chemotaxis protein cheY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-3F7N-1 RCSB PDB | xray | resolution: 2 Å | in vitro · pH 8.4 · ligands BEF, GOL, MN, SO4 Crystal Structure of CheY triple mutant F14E, N59M, E89L complexed with BeF3- and Mn2+ · Chemotaxis protein cheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3FFW-1 RCSB PDB | xray | resolution: 2 Å | in vitro · pH 8.4 · ligands BEF, GOL, MN, SO4 Crystal Structure of CheY triple mutant F14Q, N59K, E89Y complexed with BeF3- and Mn2+ · Chemotaxis protein cheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3FFX-1 RCSB PDB | xray | resolution: 2.01 Å | in vitro · pH 8.4 · ligands BEF, GOL, MN, SO4 Crystal Structure of CheY triple mutant F14E, N59R, E89H complexed with BeF3- and Mn2+ · Chemotaxis protein cheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3MYY-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · -173.15 °C · ligands BEF, GOL, MN, SO4 Structure of E. Coli CheY mutant A113P bound to Beryllium fluoride · Chemotaxis protein cheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3OLV-1 RCSB PDB | xray | resolution: 1.697 Å | in vitro · -173.15 °C · pH 8 · ligands BEF, MG, SO4 Structural and functional effects of substitution at position T+1 in CheY: CheYA88V-BeF3-Mg complex · Chemotaxis protein CheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3OLW-1 RCSB PDB | xray | resolution: 2.304 Å | in vitro · -173.15 °C · pH 7.5 · ligands BEF, CL, GOL, MN, SO4 Structural and functional effects of substitution at position T+1 in CheY: CheYA88T-BeF3-Mn complex · Chemotaxis protein CheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3OLX-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · -173.15 °C · ligands BEF, GOL, MG, MN, SO4 Structural and functional effects of substitution at position T+1 in CheY: CheYA88S-BeF3-Mn complex · Chemotaxis protein CheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3OO0-1 RCSB PDB | xray | resolution: 1.55 Å | in vitro · -173.15 °C · pH 8.25 · ligands GOL, MN, NH4, SO4 Structure of apo CheY A113P · Chemotaxis protein CheY (chains A,B) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-3OO1-1 RCSB PDB | xray | resolution: 1.7 Å | in vitro · -173.15 °C · pH 6 · ligands MG Structure of E. Coli CheY mutant A113P in the absence of Sulfate · Chemotaxis protein CheY (chains A,B) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-3RVK-1 RCSB PDB | xray | resolution: 1.16 Å | in vitro · -173.15 °C · pH 6 · ligands MN Structure of the CheY-Mn2+ Complex with substitutions at 59 and 89: N59D E89Q · Chemotaxis protein CheY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-3RVJ-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · -173.15 °C · pH 8.25 · ligands BEF, GOL, MN, SO4 Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89Q · Chemotaxis protein CheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3OLY-1 RCSB PDB | xray | resolution: 2.05 Å | in vitro · -173.15 °C · pH 8 · ligands BEF, GOL, MN, SO4 Structural and functional effects of substitution at position T+1 in CheY: CheYA88M-BeF3-Mn complex · Chemotaxis protein CheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3FGZ-1 RCSB PDB | xray | resolution: 2 Å | in vitro · pH 8.4 · ligands BEF, GOL, MN, NH4 Crystal Structure of CheY triple mutant F14E, N59M, E89R complexed with BeF3- and Mn2+ · Chemotaxis protein cheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3FFT-1 RCSB PDB | xray | resolution: 2.21 Å | in vitro · pH 8.4 · ligands BEF, GOL, MN, SO4 Crystal Structure of CheY double mutant F14E, E89R complexed with BeF3- and Mn2+ · Chemotaxis protein cheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3CHY-1 RCSB PDB | xray | resolution: 1.66 Å | in vitro · ligands SO4 CRYSTAL STRUCTURE OF ESCHERICHIA COLI CHEY REFINED AT 1.7-ANGSTROM RESOLUTION · CHEY (chains A) | inactive (unphosphorylated) · curator:draft (unreviewed) · high Unphosphorylated CheY, 1.7 Å. |
| EV-PDB-2LP4-2 RCSB PDB | nmr_structure | structure | in vitro · 24.85 °C · pH 6.5 Solution structure of P1-CheY/P2 complex in bacterial chemotaxis · Chemotaxis protein CheY (chains Y) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-2IDM-1 RCSB PDB | xray | resolution: 2 Å | in vitro · -173.15 °C · pH 5.88 · ligands ACT 2.00 A Structure of T87I/Y106W Phosphono-CheY · Chemotaxis protein cheY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-2ID7-1 RCSB PDB | xray | resolution: 1.75 Å | in vitro · -173.15 °C · pH 4.6 1.75 A Structure of T87I Phosphono-CheY · Chemotaxis protein cheY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1ZDM-1 RCSB PDB | xray | resolution: 2.4 Å | in vitro · -173.15 °C · pH 8.4 · ligands BEF, MN, XE Crystal Structure of Activated CheY Bound to Xe · Chemotaxis protein cheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-1U8T-1 RCSB PDB | xray | resolution: 1.5 Å | in vitro · -173.15 °C · pH 7.9 · ligands SO4 Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide · Chemotaxis protein cheY (chains A,B,C,D) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1MIH-1 RCSB PDB | xray | resolution: 2.7 Å | in vitro · -173.15 °C · pH 8.4 · ligands BEF, MN, SO4 A ROLE FOR CHEY GLU 89 IN CHEZ-MEDIATED DEPHOSPHORYLATION OF THE E. COLI CHEMOTAXIS RESPONSE REGULATOR CHEY · Chemotaxis protein cheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-1HEY-1 RCSB PDB | xray | resolution: 2.24 Å | in vitro INVESTIGATING THE STRUCTURAL DETERMINANTS OF THE P21-LIKE TRIPHOSPHATE AND MG2+ BINDING SITE · CHEY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1EAY-1 RCSB PDB | xray | resolution: 2 Å | in vitro · 13.85 °C · pH 7 CHEY-BINDING (P2) DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM ESCHERICHIA COLI · CHEY (chains A,B) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1E6M-1 RCSB PDB | xray | resolution: 1.7 Å | in vitro · 13.85 °C · pH 7 TWO-COMPONENT SIGNAL TRANSDUCTION SYSTEM D57A MUTANT OF CHEY · CHEMOTAXIS PROTEIN CHEY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1E6L-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · 13.85 °C · pH 7 Two-component signal transduction system D13A mutant of CheY · Chemotaxis protein CheY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1DJM-1 RCSB PDB | nmr_structure | structure | in vitro · 24.85 °C · pH 6.7 SOLUTION STRUCTURE OF BEF3-ACTIVATED CHEY FROM ESCHERICHIA COLI · CHEMOTAXIS PROTEIN Y (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1CYE-1 RCSB PDB | nmr_structure | structure | in vitro THREE DIMENSIONAL STRUCTURE OF CHEMOTACTIC CHE Y PROTEIN IN AQUEOUS SOLUTION BY NUCLEAR MAGNETIC RESONANCE METHODS · CHEY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1BDJ-1 RCSB PDB | xray | resolution: 2.68 Å | in vitro · 9.85 °C · pH 7 · ligands SO4 COMPLEX STRUCTURE OF HPT DOMAIN AND CHEY · CHEY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1AB5-1 RCSB PDB | xray | resolution: 2.4 Å | in vitro · 19.85 °C · pH 7.2 STRUCTURE OF CHEY MUTANT F14N, V21T · CHEY (chains A,B) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-1A0O-1 RCSB PDB | xray | resolution: 2.95 Å | in vitro · 19.85 °C · pH 5.5 · ligands MN CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY · CHEY (chains A,C,E,G) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-3RVL-1 RCSB PDB | xray | resolution: 1.55 Å | in vitro · -173.15 °C · pH 8.25 · ligands BEF, GOL, MN, SO4 Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89R · Chemotaxis protein CheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3RVM-1 RCSB PDB | xray | resolution: 1.45 Å | in vitro · -173.15 °C · pH 8.25 · ligands MN Structure of the CheY-Mn2+ Complex with substitutions at 59 and 89: N59D and E89R · Chemotaxis protein CheY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-3RVN-1 RCSB PDB | xray | resolution: 2.25 Å | in vitro · -173.15 °C · pH 8.25 · ligands BEF, GOL, MN, SO4 Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89Y · Chemotaxis protein CheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3RVO-1 RCSB PDB | xray | resolution: 1.55 Å | in vitro · -173.15 °C · pH 6 · ligands MN Structure of CheY-Mn2+ Complex with substitutions at 59 and 89: N59D E89Y · Chemotaxis protein CheY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-3RVP-1 RCSB PDB | xray | resolution: 2.404 Å | in vitro · -173.15 °C · pH 7.5 · ligands BEF, GOL, MG, MN, SO4 Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89K · Chemotaxis protein CheY (chains A,B) | active (BeF3- phosphoryl mimic) · rule:chey-bef3-active · high BeF3- (BEF) bound: phosphoryl mimic that activates CheY. |
| EV-PDB-3RVQ-1 RCSB PDB | xray | resolution: 1.15 Å | in vitro · -173.15 °C · pH 6 · ligands MN Structure of the CheY-Mn2+ Complex with substitutions at 59 and 89: N59D E89K · Chemotaxis protein CheY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-3RVR-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · -173.15 °C · pH 7.5 · ligands GOL, MN, MOO, SO4 Structure of the CheYN59D/E89R Molybdate complex · Chemotaxis protein CheY (chains A,B) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-5CHY-1 RCSB PDB | xray | resolution: 2 Å | in vitro · 18.85 °C · pH 6 · ligands CA STRUCTURE OF CHEMOTAXIS PROTEIN CHEY · CHEY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-6CHY-1 RCSB PDB | xray | resolution: 2.33 Å | in vitro · -193.15 °C · ligands SO4 STRUCTURE OF CHEMOTAXIS PROTEIN CHEY · CHEY (chains A,B) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-6TG7-1 RCSB PDB | xray | resolution: 1.65 Å | in vitro · -173.15 °C · pH 6 · ligands MG Crystal structure of the CheY in presence of magnesium · Chemotaxis protein CheY (chains A) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-PDB-3RVS-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · -173.15 °C · pH 7.5 · ligands GOL, MN, SO4, WO4 Structure of the CheYN59D/E89R Tungstate complex · Chemotaxis protein CheY (chains A,B) | inactive (unphosphorylated) · rule:chey-unphosphorylated · low Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active. |
| EV-BMRB-18234 BMRB | nmr_shifts | chemical_shift | in vitro · -0.15 °C · pH 6.5 Solution structure of P1-CheY/P2 complex in bacterial chemotaxis · BMRB data: 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-BMRB-2950 BMRB | nmr_shifts | chemical_shift | in vitro · 29.85 °C · pH 6.4 Assessment of cheY binding regions using 2D NMR and paramagnetic ligands · BMRB data: 1H chemical shifts | unmapped |
| EV-BMRB-2951 BMRB | nmr_shifts | chemical_shift | in vitro · 29.85 °C · pH 6.4 Assessment of cheY binding regions using 2D NMR and paramagnetic ligands · BMRB data: 1H chemical shifts | unmapped |
| EV-BMRB-3440 BMRB | nmr_shifts | chemical_shift | in vitro · 34.85 °C · pH 7 1H- and 15N-NMR assignment and solution structure of the chemotactic Escherichia coli Che Y protein · BMRB data: 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-BMRB-4083 BMRB | nmr_shifts | chemical_shift | in vitro · 29.85 °C · pH 6.75 Assignments, Secondary Structure, Global Fold, and Dynamics of Chemotaxis Y Protein Using Three- and Four-Dimensional Heteronuclear (13C, 15N) NMR Spectroscopy · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-BMRB-4472 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 6.7 1H, 13C, and 15N signal assignments for BeFx-activated CheY from E. coli · BMRB data: 1H chemical shifts, 13C chemical shifts, 15N chemical shifts | unmapped |
| EV-AFDB-AF-P0AE67-F1 AlphaFold DB | af_prediction | plddt: 97 pLDDT | in silico AlphaFold DB prediction AF-P0AE67-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-129 | unmapped |
| EV-MDDB-A0088 MDDB | md | trajectory: 10 ns | in silico · 26.85 °C MoDEL 1CHN · start 1CHN; Amber Parm99 | unmapped |
Conflicts
No conflicts found among comparable evidence.
69 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.
Downloads
- Protein bundle (JSON)
- CheY-inactive-cif (mmCIF, observed)
- CheY-inactive-pdb (PDB, observed)
- CheY-active-cif (mmCIF, observed)
- CheY-active-pdb (PDB, observed)
- Curation file (YAML)