DNA-binding protein H-NS
UniProt P0ACF8 · gene hns · Escherichia coli (strain K12) · 137 residues
19evidence items
0mapped to a state
0states
5techniques
5sources
0conflicts
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | nmr_structure | nmr_shifts | force_spectroscopy | af_prediction | md | Total |
|---|---|---|---|---|---|---|---|
| No states defined yet. | |||||||
Unmapped evidence: 19 of 19 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 0 |
| BMRB | 3 | 0 |
| MDDB | 1 | 0 |
| RCSB PDB | 5 | 0 |
| SMASH-BIO | 9 | 0 |
Structure
No state models are exported for this protein yet. Observed states export the representative PDB entry; inferred states need fitted models, not yet built.
States and transitions
No states defined.
| State | Basis | Parent | Description |
|---|
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-1NI8-1 RCSB PDB | nmr_structure | structure | in vitro · 34.85 °C · pH 6.8 H-NS dimerization motif · DNA-binding protein H-NS (chains A,B) | unmapped |
| EV-PDB-2MW2-2 RCSB PDB | nmr_structure | structure | in vitro · 21.85 °C · pH 7 Hha-H-NS46 charge zipper complex · DNA-binding protein H-NS (chains B,C) | unmapped |
| EV-PDB-1LR1-1 RCSB PDB | nmr_structure | structure | in vitro · 24.85 °C · pH 7 Solution Structure of the Oligomerization Domain of the Bacterial Chromatin-Structuring Protein H-NS · dna-binding protein h-ns (chains A,B) | unmapped |
| EV-PDB-1HNS-1 RCSB PDB | nmr_structure | structure | in vitro H-NS (DNA-BINDING DOMAIN) · H-NS (chains A) | unmapped |
| EV-PDB-1HNR-1 RCSB PDB | nmr_structure | structure | in vitro H-NS (DNA-BINDING DOMAIN) · H-NS (chains A) | unmapped |
| EV-BMRB-25296 BMRB | nmr_shifts | chemical_shift | in vitro · 21.85 °C · pH 7 Hha-H-NS46 charge zipper complex · BMRB data: 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-BMRB-4802 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 7 1H, 13C, and 15N sequential assignment of the triple labelled N-terminal domain of the Histone like Nucleoid Structuring protein (H-NS) from Salmonella typhimurium (first 64 residues of the protein) · BMRB data: 1H chemical shifts, 13C chemical shifts, 15N chemical shifts | unmapped |
| EV-BMRB-5390 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 7 1H,13C and 15N Chemical Shifts Assignments for the Oligomerization Domain of H-NS · BMRB data: 1H chemical shifts, 13C chemical shifts, 15N chemical shifts | unmapped |
| EV-AFDB-AF-P0ACF8-F1 AlphaFold DB | af_prediction | plddt: 85.81 pLDDT | in silico AlphaFold DB prediction AF-P0ACF8-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-137 | unmapped |
| EV-MDDB-A00JF MDDB | md | trajectory: 10 ns | in silico · 26.85 °C MoDEL 1HNR · start 1HNR; Amber Parm99 | unmapped |
| EV-SMASH-SMFS-002282 SMASH-BIO | force_spectroscopy | study_record | in vitro Cooperative effects on the compaction of DNA fragments by the nucleoid protein H-NS and the crowding agent PEG probed by Magnetic Tweezers. · H-NS; magnetic tweezers; experiment class: nucleic_acid_protein_interaction, unfolding_protein | unmapped |
| EV-SMASH-SMFS-003410 SMASH-BIO | force_spectroscopy | study_record | in vitro Applications of Magnetic Tweezers to Studies of NAPs. · H-NS; magnetic tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-003655 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7 Probing the mechanical stability of bridged DNA-H-NS protein complexes by single-molecule AFM pulling. · H-NS; afm smfs; experiment class: chromatin_mechanics, nucleic_acid_protein_interaction, unfolding_protein | unmapped |
| EV-SMASH-SMFS-004441 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 8 A biomechanical mechanism for initiating DNA packaging. · H-NS; optical tweezers; experiment class: chromatin_mechanics, force_generation_motor, nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-004592 SMASH-BIO | force_spectroscopy | study_record | in vitro Locus of enterocyte effacement-encoded regulator (Ler) of pathogenic Escherichia coli competes off histone-like nucleoid-structuring protein (H-NS) through noncooperative DNA binding. · H-NS; magnetic tweezers; experiment class: chromatin_mechanics, nucleic_acid_protein_interaction, rheology_cell, translation_transcription_replication, unfolding_protein | unmapped |
| EV-SMASH-SMFS-004773 SMASH-BIO | force_spectroscopy | study_record | in vitro A model of H-NS mediated compaction of bacterial DNA. · H-NS; optical tweezers; experiment class: chromatin_mechanics, nucleic_acid_protein_interaction, unfolding_protein | unmapped |
| EV-SMASH-SMFS-005633 SMASH-BIO | force_spectroscopy | study_record | in vitro Salmonella enterica response regulator SsrB relieves H-NS silencing by displacing H-NS bound in polymerization mode and directly activates transcription. · H-NS; magnetic tweezers; experiment class: force_generation_polymerization, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-006159 SMASH-BIO | force_spectroscopy | study_record | in vitro Protein-mediated molecular bridging: a key mechanism in biopolymer organization. · H-NS; optical tweezers; experiment class: chromatin_mechanics, nucleic_acid_protein_interaction, unfolding_protein | unmapped |
| EV-SMASH-SMFS-006750 SMASH-BIO | force_spectroscopy | study_record | in vitro Bacterial chromatin organization by H-NS protein unravelled using dual DNA manipulation. · H-NS; optical tweezers; experiment class: chromatin_mechanics, force_generation_motor, nucleic_acid_protein_interaction, unfolding_protein | unmapped |
Conflicts
No conflicts found among comparable evidence.
19 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.