Dihydrofolate reductase
UniProt P0ABQ4 · gene folA · Escherichia coli (strain K12) · 159 residues
192evidence items
80mapped to a state
2states
6techniques
5sources
0conflicts
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | xray | cryoem | neutron | nmr_shifts | force_spectroscopy | af_prediction | Total |
|---|---|---|---|---|---|---|---|---|
| closed (Met20 loop closed over folate and NADP+) DHFR-closed | observed (one method) | proc 73 | · | · | · | · | · | 73 |
| occluded (product-analogue complexes) DHFR-occluded | observed (one method) | proc 7 | · | · | · | · | · | 7 |
Unmapped evidence: 112 of 192 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 0 |
| BMRB | 5 | 0 |
| DisProt | 12 | 0 |
| RCSB PDB | 165 | 80 |
| SMASH-BIO | 9 | 0 |
Curated rules applied
dhfr-ddthf-occluded: 6 itemsdhfr-folate-nadp-closed: 72 items
Structure
Choose a state. Each model is badged observed or inferred; assumptions are one click away.
States and transitions
| State | Basis | Parent | Description |
|---|---|---|---|
| closed (Met20 loop closed over folate and NADP+) DHFR-closed | observed | — | Met20 loop closed over the bound folate and NADP+, a model of the Michaelis complex, as in 1RX2. |
| occluded (product-analogue complexes) DHFR-occluded | observed | — | Met20 loop occluding the nicotinamide site in complexes with the product analogue 5,10-dideazatetrahydrofolate, as in 1RX4. |
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-1DRA-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · ligands CA, CL, MTX CRYSTAL STRUCTURE OF UNLIGANDED ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE. LIGAND-INDUCED CONFORMATIONAL CHANGES AND COOPERATIVITY IN BINDING · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-1DYH-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · ligands CA, CL, DZF ISOMORPHOUS CRYSTAL STRUCTURES OF ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE, 5-DEAZAFOLATE AND 5,10-DIDEAZATETRAHYDROFOLATE: MECHANISTIC IMPLICATIONS · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-1RA2-1 RCSB PDB | xray | resolution: 1.6 Å | in vitro · 24.85 °C · pH 7 · ligands FOL, NAP DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE AND NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (OXIDIZED FORM) · DIHYDROFOLATE REDUCTASE (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-1RF7-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · 3.85 °C · pH 6.6 · ligands CL, DHF, MN STRUCTURE OF DIHYDROFOLATE REDUCTASE COMPLEXED WITH DIHYDROFOLATE · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1TDR-1 RCSB PDB | xray | resolution: 2.5 Å | in vitro · ligands CA, CL, MTX, TE EXPRESSION, CHARACTERIZATION, AND CRYSTALLOGRAPHIC ANALYSIS OF TELLUROMETHIONYL DIHYDROFOLATE REDUCTASE · TELLUROMETHIONYL DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-2D0K-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · 16.85 °C · pH 8.5 · ligands CL, FOL Methionine-free mutant of Escherichia coli dihydrofolate reductase · Dihydrofolate reductase (chains A,B) | unmapped |
| EV-PDB-2DRC-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · ligands CA, CL, MTX INVESTIGATION OF THE FUNCTIONAL ROLE OF TRYPTOPHAN-22 IN ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE BY SITE-DIRECTED MUTAGENESIS · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-3DRC-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · ligands CA, CL, MTX INVESTIGATION OF THE FUNCTIONAL ROLE OF TRYPTOPHAN-22 IN ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE BY SITE-DIRECTED MUTAGENESIS · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-3K74-1 RCSB PDB | xray | resolution: 1.95 Å | in vitro · -173.15 °C · pH 8.5 Disruption of protein dynamics by an allosteric effector antibody · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-3R33-1 RCSB PDB | xray | resolution: 2.09 Å | in vitro · -173.15 °C · pH 8 · ligands 6ME, CA, CL, NDP Evidence for dynamic motion in proteins as a mechanism for ligand dissociation · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-3QYO-1 RCSB PDB | xray | resolution: 2.09 Å | in vitro · -173.15 °C · pH 8 · ligands CA, NDP, Q24 Sensitivity of receptor internal motions to ligand binding affinity and kinetic off-rate · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-3QYL-1 RCSB PDB | xray | resolution: 1.79 Å | in vitro · -173.15 °C · pH 8 · ligands 7ME, CA, CL, NDP Sensitivity of receptor internal motions to ligand binding affinity and kinetic off-rate · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-3QL3-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · ligands FOL, MN, NAP Re-refined coordinates for PDB entry 1RX2 · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-3OCH-1 RCSB PDB | xray | resolution: 1.79 Å | in vitro · -73.15 °C · pH 6.6 · ligands 2MX, CL, MN Chemically Self-assembled Antibody Nanorings (CSANs): Design and Characterization of an Anti-CD3 IgM Biomimetic · Dihydrofolate reductase (chains A,B) | unmapped |
| EV-PDB-3KFY-1 RCSB PDB | xray | resolution: 2.08 Å | in vitro · -173.15 °C · pH 8 · ligands CA, JZM, NDP Dynamic switching and partial occupancies of a small molecule inhibitor complex of DHFR · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-3DAU-1 RCSB PDB | xray | resolution: 1.5 Å | in vitro · -173.15 °C · pH 5.5 · ligands MTX, NDP Crystal structure of the ternary MTX NADPH complex of Escherichia coli dihydrofolate reductase · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-2INQ-1 RCSB PDB | neutron | resolution: 2.2 Å | in vitro · 19.85 °C · pH 7.5 · ligands MT1 Neutron Crystal Structure of Escherichia coli Dihydrofolate Reductase Bound to the Anti-cancer drug, Methotrexate · Dihydrofolate reductase (chains A,B) | unmapped |
| EV-PDB-2ANQ-1 RCSB PDB | xray | resolution: 2.13 Å | in vitro · -173.15 °C · pH 7.5 · ligands C1A, MN, NDP Crystal Structure of E.coli DHFR in complex with NADPH and the inhibitor compound 10a. · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-2ANO-1 RCSB PDB | xray | resolution: 2.68 Å | in vitro · -173.15 °C · pH 7.6 · ligands 817, MN, NDP Crystal structure of E.coli dihydrofolate reductase in complex with NADPH and the inhibitor MS-SH08-17 · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-1RX9-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · 3.85 °C · pH 7 · ligands BME, CA, NAP DIHYDROFOLATE REDUCTASE (E.C.1.5.1.3) COMPLEXED WITH NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (OXIDIZED FORM) · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1RX8-1 RCSB PDB | xray | resolution: 2.8 Å | in vitro · 24.85 °C · pH 7 · ligands BME, FOL, MN, NAP DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE AND 2'-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE · DIHYDROFOLATE REDUCTASE (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-1RX7-1 RCSB PDB | xray | resolution: 2.3 Å | in vitro · 19.85 °C · pH 6.5 · ligands FOL STRUCTURE OF DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1RX6-1 RCSB PDB | xray | resolution: 2 Å | in vitro · 19.85 °C · pH 6 · ligands BME, DDF, NAP DIHYDROFOLATE REDUCTASE (E.C.1.5.1.3) COMPLEXED WITH 5,10-DIDEAZATETRAHYDROFOLATE AND NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (REDUCED FORM) · DIHYDROFOLATE REDUCTASE (chains A) | occluded (product-analogue complexes) · rule:dhfr-ddthf-occluded · medium Product analogue 5,10-dideazatetrahydrofolate (DDF) bound: occluded in E. coli DHFR. |
| EV-PDB-1RX5-1 RCSB PDB | xray | resolution: 2.3 Å | in vitro · 19.85 °C · pH 7.4 · ligands DDF DIHYDROFOLATE REDUCTASE (E.C.1.5.1.3) COMPLEXED WITH 5,10-DIDEAZATETRAHYDROFOLATE · DIHYDROFOLATE REDUCTASE (chains A) | occluded (product-analogue complexes) · rule:dhfr-ddthf-occluded · medium Product analogue 5,10-dideazatetrahydrofolate (DDF) bound: occluded in E. coli DHFR. |
| EV-PDB-1RX4-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · 19.85 °C · pH 6 · ligands BME, DDF, NAP DIHYDROFOLATE REDUCTASE (E.C.1.5.1.3) COMPLEXED WITH 5,10-DIDEAZATETRAHYDROFOLATE AND 2'-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE · DIHYDROFOLATE REDUCTASE (chains A) | occluded (product-analogue complexes) · curator:draft (unreviewed) · medium Dideazatetrahydrofolate complex; occluded in Sawaya and Kraut (1997). Confirm. |
| EV-PDB-1RX3-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · 19.85 °C · pH 7 · ligands BME, MN, MTX, NAP DIHYDROFOLATE REDUCTASE (E.C.1.5.1.3) COMPLEXED WITH METHOTREXATE AND NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (REDUCED FORM) · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1RX2-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · 19.85 °C · pH 7 · ligands BME, FOL, MN, NAP DIHYDROFOLATE REDUCTASE (E.C.1.5.1.3) COMPLEXED WITH WITH FOLATE AND NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (OXIDIZED FORM) · DIHYDROFOLATE REDUCTASE (chains A) | closed (Met20 loop closed over folate and NADP+) · curator:draft (unreviewed) · medium Folate and NADP+ complex; closed in Sawaya and Kraut (1997). Confirm. |
| EV-PDB-1RX1-1 RCSB PDB | xray | resolution: 2 Å | in vitro · 19.85 °C · pH 8 · ligands CA, NAP DIHYDROFOLATE REDUCTASE (E.C.1.5.1.3) COMPLEXED WITH NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (REDUCED FORM) · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1RH3-1 RCSB PDB | xray | resolution: 2.4 Å | in vitro · 24.85 °C · pH 7 · ligands MTX, NAP DIHYDROFOLATE REDUCTASE COMPLEXED WITH METHOTREXATE AND NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (REDUCED FORM) · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1RG7-1 RCSB PDB | xray | resolution: 2 Å | in vitro · 24.85 °C · pH 7.2 · ligands MTX DIHYDROFOLATE REDUCTASE COMPLEXED WITH METHOTREXATE · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1RE7-1 RCSB PDB | xray | resolution: 2.6 Å | in vitro · 24.85 °C · pH 8 · ligands FOL DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-1RD7-1 RCSB PDB | xray | resolution: 2.6 Å | in vitro · 24.85 °C · pH 8.5 · ligands BME, FOL DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-1RC4-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · 24.85 °C · pH 7.2 · ligands DDF, NAP DIHYDROFOLATE REDUCTASE COMPLEXED WITH 5,10-DIDEAZATETRAHYDROFOLATE AND NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (OXIDIZED FORM) · DIHYDROFOLATE REDUCTASE (chains A) | occluded (product-analogue complexes) · rule:dhfr-ddthf-occluded · medium Product analogue 5,10-dideazatetrahydrofolate (DDF) bound: occluded in E. coli DHFR. |
| EV-PDB-1RB3-1 RCSB PDB | xray | resolution: 2.3 Å | in vitro · 24.85 °C · pH 7 · ligands MTX, NAP DIHYDROFOLATE REDUCTASE COMPLEXED WITH METHOTREXATE AND NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (OXIDIZED FORM) · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-1RB2-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · 24.85 °C · pH 8 · ligands FOL, NAP DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE AND NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (OXIDIZED FORM) · DIHYDROFOLATE REDUCTASE (chains A,B) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-1RA9-1 RCSB PDB | xray | resolution: 1.55 Å | in vitro · 24.85 °C · pH 7 · ligands NAP DIHYDROFOLATE REDUCTASE COMPLEXED WITH NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (OXIDIZED FORM) · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1RA8-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · 24.85 °C · pH 7 · ligands ATR, FOL DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE AND 2-MONOPHOSPHOADENOSINE 5'-DIPHOSPHORIBOSE · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1RA3-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · 24.85 °C · pH 6 · ligands BME, MTX, NAP DIHYDROFOLATE REDUCTASE COMPLEXED WITH METHOTREXATE AND NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (OXIDIZED FORM) · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1RA1-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · 24.85 °C · pH 7 · ligands CA, NAP DIHYDROFOLATE REDUCTASE COMPLEXED WITH NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (REDUCED FORM) · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1JOM-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · ligands CA, CL, EOH, FFO THE CRYSTAL STRUCTURE OF THE BINARY COMPLEX BETWEEN FOLINIC ACID (LEUCOVORIN) AND E. COLI DIHYDROFOLATE REDUCTASE · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1JOL-1 RCSB PDB | xray | resolution: 1.96 Å | in vitro · ligands CA, CL, FFO THE CRYSTAL STRUCTURE OF THE BINARY COMPLEX BETWEEN FOLINIC ACID (LEUCOVORIN) AND E. COLI DIHYDROFOLATE REDUCTASE · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-1DYJ-1 RCSB PDB | xray | resolution: 1.85 Å | in vitro · ligands CA, CL, DDF ISOMORPHOUS CRYSTAL STRUCTURES OF ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE, 5-DEAZAFOLATE AND 5,10-DIDEAZATETRAHYDROFOLATE: MECHANISTIC IMPLICATIONS · DIHYDROFOLATE REDUCTASE (chains A,B) | occluded (product-analogue complexes) · rule:dhfr-ddthf-occluded · medium Product analogue 5,10-dideazatetrahydrofolate (DDF) bound: occluded in E. coli DHFR. |
| EV-PDB-1DYI-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · ligands CA, CL, FOL ISOMORPHOUS CRYSTAL STRUCTURES OF ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE, 5-DEAZAFOLATE AND 5,10-DIDEAZATETRAHYDROFOLATE: MECHANISTIC IMPLICATIONS · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-1DRH-1 RCSB PDB | xray | resolution: 2.3 Å | in vitro · ligands NAP ISOMORPHOUS CRYSTAL STRUCTURES OF ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE, 5-DEAZAFOLATE AND 5,10-DIDEAZATETRAHYDROFOLATE: MECHANISTIC IMPLICATIONS · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1DRE-1 RCSB PDB | xray | resolution: 2.6 Å | in vitro · 24.85 °C · pH 6 · ligands MTX, NAP DIHYDROFOLATE REDUCTASE COMPLEXED WITH METHOTREXATE AND NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE (OXIDIZED FORM) · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-1DRB-1 RCSB PDB | xray | resolution: 1.96 Å | in vitro · ligands CA, CL, EOH, MTX CRYSTAL STRUCTURE OF UNLIGANDED ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE. LIGAND-INDUCED CONFORMATIONAL CHANGES AND COOPERATIVITY IN BINDING · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-1DHJ-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · ligands CA, CL, MTX LONG-RANGE STRUCTURAL EFFECTS IN A SECOND-SITE REVERTANT OF A MUTANT DIHYDROFOLATE REDUCTASE · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-1DHI-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · ligands CA, CL, MTX LONG-RANGE STRUCTURAL EFFECTS IN A SECOND-SITE REVERTANT OF A MUTANT DIHYDROFOLATE REDUCTASE · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-1DDS-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · ligands CA, CL, MTX MOLECULE: DIHYDROFOLATE REDUCTASE (E.C.1.5.1.3) COMPLEXED WITH METHOTREXATE · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-1DDR-1 RCSB PDB | xray | resolution: 2.45 Å | in vitro · ligands CA, CL, MTX, URE MOLECULE: DIHYDROFOLATE REDUCTASE (E.C.1.5.1.3) COMPLEXED WITH METHOTREXATE AND UREA · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-45PC-1 RCSB PDB | xray | resolution: 0.85 Å | in vitro · -173.15 °C · pH 7 · ligands CL, FOL, MN, NAP 6-conformation model of Escherichia coli dihydrofolate reductase at 100K · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-4DFR-1 RCSB PDB | xray | resolution: 1.7 Å | in vitro · ligands CA, CL, MTX CRYSTAL STRUCTURES OF ESCHERICHIA COLI AND LACTOBACILLUS CASEI DIHYDROFOLATE REDUCTASE REFINED AT 1.7 ANGSTROMS RESOLUTION. I. GENERAL FEATURES AND BINDING OF METHOTREXATE · DIHYDROFOLATE REDUCTASE (chains A,B) | unmapped |
| EV-PDB-4EJ1-1 RCSB PDB | xray | resolution: 1.75 Å | in vitro · -173.15 °C · pH 4.4 · ligands FOL, PO4 Binding of Nb113 camelid antibody fragment with the binary DHFR:folate complex · Dihydrofolate reductase (chains A,B) | unmapped |
| EV-PDB-4FHB-1 RCSB PDB | xray | resolution: 2.8 Å | in vitro · -173.15 °C · pH 7.5 · ligands FOL, NAP Enhancing DHFR catalysis by binding of an allosteric regulator nanobody (Nb179) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-4GH8-1 RCSB PDB | xray | resolution: 1.85 Å | in vitro · -173.15 °C · pH 6.5 · ligands CA, MTX, NDP Crystal structure of a 'humanized' E. coli dihydrofolate reductase · Dihydrofolate reductase (chains A,B) | unmapped |
| EV-PDB-4I13-1 RCSB PDB | xray | resolution: 1.6 Å | in vitro · -173.15 °C · pH 7 · ligands FOL Nanobody ca1697 binding to the DHFR.folate binary complex · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-4I1N-1 RCSB PDB | xray | resolution: 1.888 Å | in vitro · -173.15 °C · pH 6.5 · ligands FOL R104A-ca1697 nanobody binding to the binary DHFR.folate complex · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-4NX6-1 RCSB PDB | xray | resolution: 1.35 Å | in vitro · -0.15 °C · pH 7.5 · ligands BME, FOL, MN, NAP single room temperature model of DHFR · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-4NX7-1 RCSB PDB | xray | resolution: 1.15 Å | in vitro · -173.15 °C · pH 7.5 · ligands BME, FOL, MN, NAP single cryogenic temperature model of DHFR · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-4PDJ-1 RCSB PDB | xray | resolution: 1.599 Å | in vitro · 17.85 °C · pH 7 · ligands DHF, MN, NDP Neutron crystal Structure of E.coli Dihydrofolate Reductase complexed with folate and NADP+ · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-4X5F-1 RCSB PDB | xray | resolution: 1.7 Å | in vitro · 24.85 °C · ligands BME, FOL, NAP ecDHFR complexed with folate and NADP+ at 0.1 MPa · Dihydrofolate reductase (chains A,B) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-4X5G-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · 24.85 °C · ligands BME, FOL, NAP ecDHFR complexed with folate and NADP+ at 270 MPa · Dihydrofolate reductase (chains A,B) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-4X5H-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · 24.85 °C · ligands BME, FOL, NAP ecDHFR complexed with folate and NADP+ at 500 MPa · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-4X5J-1 RCSB PDB | xray | resolution: 1.85 Å | in vitro · 24.85 °C · ligands BME, FOL, NAP ecDHFR complexed with folate and NADP+ at 750 MPa · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-5CC9-1 RCSB PDB | xray | resolution: 1.199 Å | in vitro · -173.15 °C · pH 7 · ligands DDF, NAP L28F E.coli dihydrofolate reductase complexed with 5,10-dideazatetrahydrofolate and oxidized nicotinamide adenine dinucleotide phosphate · Dihydrofolate reductase (chains A) | occluded (product-analogue complexes) · rule:dhfr-ddthf-occluded · medium Product analogue 5,10-dideazatetrahydrofolate (DDF) bound: occluded in E. coli DHFR. |
| EV-PDB-5CCC-1 RCSB PDB | xray | resolution: 1.5 Å | in vitro · -173.15 °C · pH 7 · ligands DDF, NAP wild-type E.coli dihydrofolate reductase complexed with 5,10-dideazatetrahydrofolate and oxidized nicotinamide adenine dinucleotide phosphate · Dihydrofolate reductase (chains A) | occluded (product-analogue complexes) · rule:dhfr-ddthf-occluded · medium Product analogue 5,10-dideazatetrahydrofolate (DDF) bound: occluded in E. coli DHFR. |
| EV-PDB-5DFR-1 RCSB PDB | xray | resolution: 2.3 Å | in vitro · ligands CL CRYSTAL STRUCTURE OF UNLIGANDED ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE. LIGAND-INDUCED CONFORMATIONAL CHANGES AND COOPERATIVITY IN BINDING · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-5EAJ-1 RCSB PDB | xray | resolution: 1.701 Å | in vitro · -173.15 °C · pH 7.5 · ligands CA, CL, FOL Crystal structure of DHFR in 0% Isopropanol · Dihydrofolate reductase (chains A,B) | unmapped |
| EV-PDB-5SSS-1 RCSB PDB | xray | resolution: 1.14 Å | in vitro · -3.15 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 270 K (crystal 1) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-5SST-1 RCSB PDB | xray | resolution: 1.07 Å | in vitro · -3.15 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 270 K (crystal 2) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-5SSW-1 RCSB PDB | xray | resolution: 1.06 Å | in vitro · -3.15 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 270 K (multi-crystal) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-5UIH-1 RCSB PDB | xray | resolution: 1.647 Å | in vitro · -173.15 °C · ligands 8CV, BME, EDO, NA, NAP structure of DHFR with bound phenformin and NADP · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-5UII-1 RCSB PDB | xray | resolution: 1.351 Å | in vitro · -173.15 °C · pH 6.8 · ligands BFR, CA, NA, NAP structure of DHFR with bound buformin and NADP · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-5W3Q-1 RCSB PDB | xray | resolution: 1.401 Å | in vitro · -173.15 °C · ligands CA, NDP L28F E.coli DHFR in complex with NADPH · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-5Z6F-1 RCSB PDB | xray | resolution: 1.801 Å | in vitro · 23.85 °C · ligands FOL, NAP High-pressure Crystal Structure Analysis of DHFR(0.1 MPa) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-5Z6M-1 RCSB PDB | xray | resolution: 2.202 Å | in vitro · 23.85 °C · ligands FOL, NAP High-pressure Crystal Structure Analysis of M20 loop closed DHFR at 800 MPa · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-6CQA-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · -173.15 °C · pH 3.5 · ligands PQD, SO4 E. coli DHFR complex with inhibitor AMPQD · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-6CW7-1 RCSB PDB | xray | resolution: 1.03 Å | in vitro · -173.15 °C · pH 6.5 · ligands CL, MG, THG E. coli DHFR product complex with (6S)-5,6,7,8-TETRAHYDROFOLATE · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-6CXK-1 RCSB PDB | xray | resolution: 1.108 Å | in vitro · -173.15 °C · pH 6.5 · ligands CL, DHF, MG E. coli DHFR substrate complex with Dihydrofolate · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-6CYV-1 RCSB PDB | xray | resolution: 1.3 Å | in vitro · -173.15 °C · pH 8 · ligands DHF, NAP E. coli DHFR ternary complex with NADP and dihydrofolate · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-6MR9-1 RCSB PDB | xray | resolution: 1.35 Å | in vitro · -173.15 °C · pH 6.5 · ligands CL, MG, THG E. coli DHFR complex with a reaction intermediate · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-6MT8-1 RCSB PDB | xray | resolution: 1.35 Å | in vitro · -173.15 °C · pH 6.5 · ligands CL, DHF, MG, THG E. coli DHFR complex modeled with two ligand states · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-6MTH-1 RCSB PDB | xray | resolution: 1.35 Å | in vitro · -173.15 °C · pH 6.5 · ligands CL, DHF, MG, THG E. coli DHFR complex modeled with three ligand states · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-6RUL-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · -173.15 °C · ligands NDP, PGE, TOP Crystal structure of GFP-LAMA-F98 - a GFP enhancer nanobody with cpDHFR insertion and TMP and NADPH · GFP-LAMA-F98 a GFP enhancer nanobody with cpDHFR insertion,Dihydrofolate reductase,GFP-LAMA-F98 a GFP enhancer nanobody with cpDHFR insertion,Dihydrofolate reductase,Dihydrofolate reductase,GFP-LAMA-F98 a GFP enhancer nanobody with cpDHFR insertion,Dihydrofolate reductase,GFP-LAMA-F98 a GFP enhancer nanobody with cpDHFR insertion (chains A) | unmapped |
| EV-PDB-6DFR-1 RCSB PDB | xray | resolution: 2.4 Å | in vitro · ligands CA, NAP CRYSTAL STRUCTURES OF ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE. THE NADP+ HOLOENZYME AND THE FOLATE(DOT)NADP+ TERNARY COMPLEX. SUBSTRATE BINDING AND A MODEL FOR THE TRANSITION STATE · DIHYDROFOLATE REDUCTASE (chains A) | unmapped |
| EV-PDB-5Z6L-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · 23.85 °C · ligands FOL, NAP High-pressure Crystal Structure Analysis of M20 loop closed DHFR at 650 MPa · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-5Z6K-1 RCSB PDB | xray | resolution: 1.802 Å | in vitro · 23.85 °C · ligands FOL, NAP High-pressure Crystal Structure Analysis of M20 loop closed DHFR at 400 MPa · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-5Z6J-1 RCSB PDB | xray | resolution: 1.803 Å | in vitro · 23.85 °C · ligands FOL, NAP High-pressure Crystal Structure Analysis of M20 loop closed DHFR at 220 MPa · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-5UJX-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · -173.15 °C · pH 7.5 · ligands CA, CL, FOL, IPA Crystal structure of DHFR in 20% Isopropanol · Dihydrofolate reductase (chains A,B) | unmapped |
| EV-PDB-5UIP-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · -173.15 °C · ligands 8DM, LG3, NA, NAP structure of DHFR with bound DAP, p-ABG and NADP · Dihydrofolate reductase (chains A,B) | unmapped |
| EV-PDB-5UIO-1 RCSB PDB | xray | resolution: 1.929 Å | in vitro · -173.15 °C · ligands 8DM, BME, FMT, LG3, NAP structure of DHFR with bound DAP, p-ABG and NADP · Dihydrofolate reductase (chains A,B,C,D,E) | unmapped |
| EV-PDB-5SSV-1 RCSB PDB | xray | resolution: 1.08 Å | in vitro · -3.15 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 270 K (crystal 4) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-5SSU-1 RCSB PDB | xray | resolution: 1.12 Å | in vitro · -3.15 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 270 K (crystal 3) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-5E8Q-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · -173.15 °C · pH 7.5 · ligands CA, CL, FOL, IPA Crystal structure of DHFR in 20% Isopropanol · Dihydrofolate reductase (chains A,B) | unmapped |
| EV-PDB-4X5I-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · 24.85 °C · ligands BME, FOL, NAP ecDHFR complexed with folate and NADP+ at 660 MPa · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-4KJL-1 RCSB PDB | xray | resolution: 1.38 Å | in vitro · -0.15 °C · pH 7.5 · ligands FOL, NAP Room Temperature N23PPS148A DHFR · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-4KJK-1 RCSB PDB | xray | resolution: 1.351 Å | in vitro · -0.15 °C · pH 7.5 · ligands CA, FOL, NAP Room Temperature WT DHFR · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-4KJJ-1 RCSB PDB | xray | resolution: 1.15 Å | in vitro · -173.15 °C · pH 7.5 · ligands FOL, NAP Cryogenic WT DHFR · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-4EIZ-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · -173.15 °C · pH 5.6 Structure of Nb113 bound to apoDHFR · Dihydrofolate reductase (chains A,B) | unmapped |
| EV-PDB-4EIG-1 RCSB PDB | xray | resolution: 2.5 Å | in vitro · -173.15 °C · pH 7.5 CA1698 camel antibody fragment in complex with DHFR · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-6RUM-1 RCSB PDB | xray | resolution: 1.6 Å | in vitro · -173.15 °C · ligands CL, NDP, PEG, PG4, TOP Crystal structure of GFP-LAMA-G97 - a GFP enhancer nanobody with cpDHFR insertion and TMP and NADPH · GFP-LAMA-G97 a GFP enhancer nanobody with cpDHFR insertion (chains A) | unmapped |
| EV-PDB-6XG4-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · -173.15 °C · pH 5.6 · ligands CL, GOL, NDP, TOP X-ray structure of Escherichia coli dihydrofolate reductase L28R mutant in complex with trimethoprim · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-6XG5-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · -173.15 °C · pH 5.6 · ligands CL, GOL, NDP, TOP X-ray structure of Escherichia coli dihydrofolate reductase in complex with trimethoprim · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-7D3Z-1 RCSB PDB | xray | resolution: 1.65 Å | in vitro · 17.85 °C · pH 4.5 · ligands FOL, NAP X-ray crystal Structure of E.coli Dihydrofolate Reductase complexed with folate and NADP+ at pH4.5 · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7D49-1 RCSB PDB | xray | resolution: 1.651 Å | in vitro · 17.85 °C · pH 4.5 · ligands FOL, NAP X-ray crystal Structure of E.coli Dihydrofolate Reductase complexed with folate and NADP+ at pH4.5 · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7D4L-1 RCSB PDB | xray | resolution: 1.6 Å | in vitro · 17.85 °C · pH 7 · ligands FOL, MN, NAP X-ray crystal Structure of E.coli Dihydrofolate Reductase complexed with folate and NADP+ at pH7.0 · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7D4X-1 RCSB PDB | xray | resolution: 1.599 Å | in vitro · 17.85 °C · pH 7 · ligands FOL, NAP X-ray crystal Structure of E.coli Dihydrofolate Reductase complexed with folate and NADP+ at pH7.0 · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7D6G-1 RCSB PDB | xray | resolution: 1.65 Å | in vitro · 17.85 °C · pH 4.5 · ligands FOL, MN, NAP Neutron crystal Structure of E.coli Dihydrofolate Reductase complexed with folate and NADP+ at pH4.5 · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7F3B-1 RCSB PDB | xray | resolution: 2.81 Å | in vitro · -173.15 °C · pH 3.5 · ligands D4C, GOL cocrystallization of Escherichia coli dihydrofolate reductase (DHFR) and its pyrrolo[3,2-f]quinazoline inhibitor. · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-7FPL-1 RCSB PDB | xray | resolution: 1.17 Å | in vitro · 6.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 280 K (crystal 1) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPM-1 RCSB PDB | xray | resolution: 1.04 Å | in vitro · 6.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 280 K (crystal 2) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPN-1 RCSB PDB | xray | resolution: 1.04 Å | in vitro · 6.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 280 K (crystal 3) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPO-1 RCSB PDB | xray | resolution: 1.06 Å | in vitro · 6.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 280 K (crystal 4) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPP-1 RCSB PDB | xray | resolution: 1.03 Å | in vitro · 6.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 280 K (crystal 5) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPQ-1 RCSB PDB | xray | resolution: 1.03 Å | in vitro · 6.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 280 K (multi-crystal) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPR-1 RCSB PDB | xray | resolution: 1.07 Å | in vitro · 16.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 290 K (crystal 1) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPS-1 RCSB PDB | xray | resolution: 1.26 Å | in vitro · 16.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 290 K (crystal 2) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPT-1 RCSB PDB | xray | resolution: 1.07 Å | in vitro · 16.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 290 K (crystal 3) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPU-1 RCSB PDB | xray | resolution: 1.05 Å | in vitro · 16.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 290 K (crystal 4) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPV-1 RCSB PDB | xray | resolution: 1.04 Å | in vitro · 16.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 290 K (crystal 5) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPW-1 RCSB PDB | xray | resolution: 1.04 Å | in vitro · 16.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 290 K (multi-crystal) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPX-1 RCSB PDB | xray | resolution: 1.06 Å | in vitro · 26.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 300 K (crystal 1) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPY-1 RCSB PDB | xray | resolution: 1.12 Å | in vitro · 26.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 300 K (crystal 2) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FPZ-1 RCSB PDB | xray | resolution: 1.32 Å | in vitro · 26.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 300 K (crystal 3) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQ0-1 RCSB PDB | xray | resolution: 1.15 Å | in vitro · 26.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 300 K (crystal 4) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQ1-1 RCSB PDB | xray | resolution: 1.18 Å | in vitro · 26.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 300 K (crystal 5) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQ2-1 RCSB PDB | xray | resolution: 1.07 Å | in vitro · 26.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 300 K (multi-crystal) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQ3-1 RCSB PDB | xray | resolution: 1.3 Å | in vitro · 36.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 310 K (crystal 1) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQ4-1 RCSB PDB | xray | resolution: 1.33 Å | in vitro · 36.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 310 K (crystal 2) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQ5-1 RCSB PDB | xray | resolution: 1.35 Å | in vitro · 36.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 310 K (crystal 3) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQ6-1 RCSB PDB | xray | resolution: 1.29 Å | in vitro · 36.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex at 310 K (multi-crystal) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQ7-1 RCSB PDB | xray | resolution: 1.1 Å | in vitro · 21.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex (crystal 1, pass 1, 295 K) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQ8-1 RCSB PDB | xray | resolution: 1.21 Å | in vitro · 36.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex (crystal 1, pass 2, 310 K) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQ9-1 RCSB PDB | xray | resolution: 1.11 Å | in vitro · 21.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex (crystal 1, pass 3, 295 K) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQA-1 RCSB PDB | xray | resolution: 1.08 Å | in vitro · 6.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex (crystal 1, pass 4, 280 K) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQB-1 RCSB PDB | xray | resolution: 1.14 Å | in vitro · 21.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex (crystal 1, pass 5, 295 K) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQC-1 RCSB PDB | xray | resolution: 1.18 Å | in vitro · 21.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex (crystal 2, pass 1, 295 K) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQD-1 RCSB PDB | xray | resolution: 1.17 Å | in vitro · 6.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex (crystal 2, pass 2, 280 K) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQE-1 RCSB PDB | xray | resolution: 1.18 Å | in vitro · 21.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex (crystal 2, pass 3, 295 K) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQF-1 RCSB PDB | xray | resolution: 1.23 Å | in vitro · 36.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex (crystal 2, pass 4, 310 K) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7FQG-1 RCSB PDB | xray | resolution: 1.19 Å | in vitro · 21.85 °C · pH 5.6 · ligands FOL, MN, NAP DHFR:NADP+:FOL complex (crystal 2, pass 5, 295 K) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7LVC-1 RCSB PDB | xray | resolution: 1.7 Å | in vitro · 21.85 °C · ligands FOL, MN, NAP E. coli DHFR by Native Mn,P,S-SAD at Room Temperature · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7MQP-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · -173.15 °C · ligands SO4, ZM4 E. coli dihydrofolate reductase complexed with 4'-chloro-3'-(4-(2,4-diamino-6-ethylpyrimidin-5-yl)but-3-yn-2-yl)-[1,1'-biphenyl]-4-carboxamide (UCP1228) · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-7MYM-1 RCSB PDB | xray | resolution: 3.04 Å | in vitro · -173.15 °C · ligands ARG, NAP, SO4, TOP Crystal structure of Escherichia coli dihydrofolate reductase in complex with TRIMETHOPRIM and NADPH · Dihydrofolate reductase (chains A,B,C) | unmapped |
| EV-PDB-7NAE-1 RCSB PDB | xray | resolution: 2.35 Å | in vitro · -173.15 °C · ligands SO4, TOP Crystal structure of Escherichia coli dihydrofolate reductase in complex with TRIMETHOPRIM · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-7REB-1 RCSB PDB | xray | resolution: 1.91 Å | in vitro · -173.15 °C · ligands 560, SO4 E. coli dihydrofolate reductase complexed with 5-(3-(7-(4-(aminomethyl)phenyl)benzo[d][1,3]dioxol-5-yl)but-1-yn-1-yl)-6-ethylpyrimidine-2,4-diamine (UCP1223) · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-8DAI-1 RCSB PDB | xray | resolution: 1.14 Å | in vitro · 11.85 °C · ligands MN, NAP, R6F E. coli DHFR complex with NADP+ and 10-methylfolate · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-8G4Z-1 RCSB PDB | xray | resolution: 1.7 Å | in vitro · 4.85 °C · ligands FOL, MN, NAP E. coli DHFR complex with NADP+ and folate: EF-X off model by Laue diffraction (no electric field) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-8G50-1 RCSB PDB | xray | resolution: 1.7 Å | in vitro · 4.85 °C · ligands FOL, MN, NAP E. coli DHFR complex with NADP+ and folate: EF-X excited state model by Laue diffraction (electric field along b axis; 8-fold extrapolation of structure factor differences) · Dihydrofolate reductase (chains A,B) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-7DFR-1 RCSB PDB | xray | resolution: 2.5 Å | in vitro · ligands FOL, NAP CRYSTAL STRUCTURES OF ESCHERICHIA COLI DIHYDROFOLATE REDUCTASE. THE NADP+ HOLOENZYME AND THE FOLATE(DOT)NADP+ TERNARY COMPLEX. SUBSTRATE BINDING AND A MODEL FOR THE TRANSITION STATE · DIHYDROFOLATE REDUCTASE (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-8HCO-6 RCSB PDB | cryoem | resolution: 4.1 Å | in vitro Substrate-engaged TOM complex from yeast · sfGFP (chains G) | unmapped |
| EV-PDB-8UW0-1 RCSB PDB | xray | resolution: 0.93 Å | in vitro · -173.15 °C · pH 7 · ligands FOL, MN, NAP Escherichia coli DHFR bound to NADP+ and folate, 17.2 MGy dose · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-8V9R-2 RCSB PDB | cryoem | resolution: 2.8 Å | in vitro · ligands ADP, ATP, MG, MTX Cryo-EM Structure of a Proteolytic ClpXP AAA+ Machine Poised to Unfold a Branched-Degron DHFR-ssrA Substrate Bound with MTX · Dihydrofolate reductase (chains S) | unmapped |
| EV-PDB-8VZ4-1 RCSB PDB | xray | resolution: 1.25 Å | in vitro · -173.15 °C · pH 8 · ligands NAP L54G Mutant of E. coli Dihydrofolate Reductase Complexed with Nicotinamide Adenine Dinucleotide Phosphate (oxidized form) · Dihydrofolate reductase (chains A,B) | unmapped |
| EV-PDB-9C87-2 RCSB PDB | cryoem | resolution: 3.7 Å | in vitro · ligands ADP, ATP, MG, MTX Cryo-EM Structure of a Proteolytic ClpXP AAA+ Machine Poised to Unfold a Linear-Degron DHFR-ssrA Substrate Bound with MTX · Dihydrofolate reductase (chains S) | unmapped |
| EV-PDB-9DAQ-1 RCSB PDB | xray | resolution: 2.35 Å | in vitro · -173.15 °C · pH 4.6 · ligands A1A3E Structure of E. coli dihydrofolate reductase (DHFR) in an occluded conformation and in complex with a cycloguanil derivative · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-9DAR-1 RCSB PDB | xray | resolution: 2.17 Å | in vitro · -173.15 °C · ligands 1CY, FLC Structure of E. coli dihydrofolate reductase (DHFR) in an occluded conformation and in complex with cycloguanil · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-9KZ4-1 RCSB PDB | xray | resolution: 1.708 Å | in vitro · -173.15 °C · ligands A1EHX, MG, NDP Dihydrofolate reductase binding to NADPH and trimethoprim-tetramethylrhodamine · Dihydrofolate reductase (chains A,B) | unmapped |
| EV-PDB-9NJO-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · -173.15 °C · pH 7 · ligands MN Y100F Mutant of E. coli Dihydrofolate Reductase · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-9PWK-1 RCSB PDB | xray | resolution: 1.51 Å | in vitro · -173.15 °C · pH 8 · ligands CA, FOL, NAP P21A Mutant of E. coli Dihydrofolate Reductase Complexed with Folate and Nicotinamide Adenine Dinucleotide Phosphate (oxidized form) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-9PWM-1 RCSB PDB | xray | resolution: 0.89 Å | in vitro · -173.15 °C · pH 7 · ligands FOL, MN, NAP Dihydrofolate Reductase Complexed with Folate and Nicotinamide Adenine Dinucleotide Phosphate (Oxidized Form) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-9ZHM-1 RCSB PDB | xray | resolution: 1.07 Å | in vitro · -173.15 °C · pH 7 · ligands FOL, MN, NAP M20E Mutant of E. coli Dihydrofolate Reductase Complexed with Nicotinamide Adenine Dinucleotide Phosphate (oxidized form) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-9PXD-1 RCSB PDB | xray | resolution: 1.07 Å | in vitro · -173.15 °C · pH 7 · ligands FOL, MN, NAP M20A Mutant of E. coli Dihydrofolate Reductase Complexed with Folate and Nicotinamide Adenine Dinucleotide Phosphate (oxidized form) · Dihydrofolate reductase (chains A) | closed (Met20 loop closed over folate and NADP+) · rule:dhfr-folate-nadp-closed · medium Folate with NADP+ bound: the Michaelis-complex mimic, closed in E. coli DHFR. |
| EV-PDB-9PXC-1 RCSB PDB | xray | resolution: 2.37 Å | in vitro · -173.15 °C · pH 7 · ligands CA, NAP Y100F Mutant of E. coli Dihydrofolate Reductase Complexed with NADP+ (oxidized form) · Dihydrofolate reductase (chains A) | unmapped |
| EV-PDB-8UCX-1 RCSB PDB | xray | resolution: 1.57 Å | in vitro · -173.15 °C · pH 7 · ligands FOL, MN Dihydrofolate Reductase Complexed with Folate · Dihydrofolate reductase (chains A) | unmapped |
| EV-BMRB-11492 BMRB | nmr_shifts | chemical_shift | in vitro · 34.85 °C · pH 6.8 Dihydrofolate Reductase from E.coli G67 deletion mutant · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-BMRB-25019 BMRB | nmr_shifts | chemical_shift | in vitro · 34.85 °C · pH 7 Sequential backbone HN, N, Ca and Cb assignments of E.coli dihydrofolate reductase-folate binary complex at pH 7.0 and 308 K · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-BMRB-4554 BMRB | nmr_shifts | chemical_shift | in vitro · 8.85 °C · pH 7.6 Backbone HN, N, Ca, C' and Cb assignments of the 19 kDa DHFR/NADPH complex at 9C and pH 7.6 · BMRB data: 1H chemical shifts, 13C chemical shifts, 15N chemical shifts | unmapped |
| EV-BMRB-5740 BMRB | nmr_shifts | chemical_shift | in vitro · 33.25 °C Backbone 1H, 13C and 15N Chemical shift assignments for the ternary DHFR:FOLATE:NADP+ complex · BMRB data: 1H chemical shifts, 13C chemical shifts, 15N chemical shifts | unmapped |
| EV-BMRB-5741 BMRB | nmr_shifts | chemical_shift | in vitro · 33.25 °C Backbone 1NH, 13C and 15N Chemical shift assignments for the ternary DHFR:FOLATE:DHNADPH complex · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-DISPROT-DP00301r001 DisProt | xray | disorder_region: 10 residues | in vitro DisProt DP00301: disorder, residues 63-72 · residues 63-72; evidence: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion | unmapped |
| EV-DISPROT-DP00301r003 DisProt | xray | disorder_region: 10 residues | in vitro DisProt DP00301: disorder to order, residues 63-72 · residues 63-72; evidence: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion | unmapped |
| EV-DISPROT-DP00301r005 DisProt | nmr_shifts | disorder_region: 10 residues | in vitro DisProt DP00301: disorder, residues 63-72 · residues 63-72; evidence: nuclear magnetic resonance spectroscopy evidence used in manual assertion | unmapped |
| EV-DISPROT-DP00301r007 DisProt | nmr_shifts | disorder_region: 10 residues | in vitro DisProt DP00301: disorder to order, residues 63-72 · residues 63-72; evidence: nuclear magnetic resonance spectroscopy evidence used in manual assertion | unmapped |
| EV-DISPROT-DP00301r009 DisProt | xray | disorder_region: 17 residues | in vitro DisProt DP00301: disorder, residues 116-132 · residues 116-132; evidence: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion | unmapped |
| EV-DISPROT-DP00301r011 DisProt | xray | disorder_region: 17 residues | in vitro DisProt DP00301: disorder to order, residues 116-132 · residues 116-132; evidence: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion | unmapped |
| EV-DISPROT-DP00301r013 DisProt | nmr_shifts | disorder_region: 17 residues | in vitro DisProt DP00301: disorder, residues 116-132 · residues 116-132; evidence: nuclear magnetic resonance spectroscopy evidence used in manual assertion | unmapped |
| EV-DISPROT-DP00301r015 DisProt | nmr_shifts | disorder_region: 17 residues | in vitro DisProt DP00301: disorder to order, residues 116-132 · residues 116-132; evidence: nuclear magnetic resonance spectroscopy evidence used in manual assertion | unmapped |
| EV-DISPROT-DP00301r017 DisProt | nmr_shifts | disorder_region: 16 residues | in vitro DisProt DP00301: disorder, residues 9-24 · residues 9-24; evidence: nuclear magnetic resonance spectroscopy evidence used in manual assertion | unmapped |
| EV-DISPROT-DP00301r019 DisProt | nmr_shifts | disorder_region: 16 residues | in vitro DisProt DP00301: disorder to order, residues 9-24 · residues 9-24; evidence: nuclear magnetic resonance spectroscopy evidence used in manual assertion | unmapped |
| EV-DISPROT-DP00301r021 DisProt | xray | disorder_region: 16 residues | in vitro DisProt DP00301: disorder, residues 9-24 · residues 9-24; evidence: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion | unmapped |
| EV-DISPROT-DP00301r023 DisProt | xray | disorder_region: 16 residues | in vitro DisProt DP00301: disorder to order, residues 9-24 · residues 9-24; evidence: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion | unmapped |
| EV-AFDB-AF-P0ABQ4-F1 AlphaFold DB | af_prediction | plddt: 96.44 pLDDT | in silico AlphaFold DB prediction AF-P0ABQ4-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-159 | unmapped |
| EV-SMASH-SMFS-000098-r0 SMASH-BIO | force_spectroscopy | distance_to_transition_state: 2.7 nm | in vitro · pH 7.2 Evolution of N-terminal mechanical lability as a determinant for Type III secretion. · Dihydrofolate reductase; afm smfs; experiment class: receptor_ligand_adhesion, unfolding_protein | unmapped |
| EV-SMASH-SMFS-000098-r1 SMASH-BIO | force_spectroscopy | distance_to_transition_state: 0.7 nm | in vitro · pH 7.2 Evolution of N-terminal mechanical lability as a determinant for Type III secretion. · Dihydrofolate reductase; afm smfs; experiment class: receptor_ligand_adhesion, unfolding_protein | unmapped |
| EV-SMASH-SMFS-000355-r0 SMASH-BIO | force_spectroscopy | distance_to_transition_state: 2.7 nm | in vitro A Type III secretion system effector evolved to be mechanically labile and initiate unfolding from the N-terminus · Dihydrofolate reductase; force spectroscopy unspecified; experiment class: receptor_ligand_adhesion, unfolding_protein | unmapped |
| EV-SMASH-SMFS-000648 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Trigger factor accelerates nascent chain compaction and folding. · Dihydrofolate reductase; optical tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication, unfolding_protein | unmapped |
| EV-SMASH-SMFS-002196-r0 SMASH-BIO | force_spectroscopy | distance_to_transition_state: 1.5 nm | in vitro Type III secretion system effector proteins are mechanically labile. · Dihydrofolate reductase; afm smfs; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-002196-r1 SMASH-BIO | force_spectroscopy | distance_to_transition_state: 0.4 nm | in vitro Type III secretion system effector proteins are mechanically labile. · Dihydrofolate reductase; afm smfs; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-005635 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 Self-assembled enzymatic monolayer directly bound to a gold surface: activity and molecular recognition force spectroscopy studies. · Dihydrofolate reductase; afm smfs; experiment class: receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-006999 SMASH-BIO | force_spectroscopy | study_record | in vitro Influence of substrate binding on the mechanical stability of mouse dihydrofolate reductase. · Dihydrofolate reductase; afm smfs; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-007002 SMASH-BIO | force_spectroscopy | study_record | in vitro Ligand binding modulates the mechanical stability of dihydrofolate reductase. · Dihydrofolate reductase; afm smfs; experiment class: receptor_ligand_adhesion, unfolding_protein | unmapped |
Conflicts
No conflicts found among comparable evidence.
192 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.
Downloads
- Protein bundle (JSON)
- DHFR-closed-cif (mmCIF, observed)
- DHFR-closed-pdb (PDB, observed)
- DHFR-occluded-cif (mmCIF, observed)
- DHFR-occluded-pdb (PDB, observed)
- Curation file (YAML)