Tropomyosin alpha-1 chain
UniProt P09493 · gene TPM1 · Homo sapiens · 284 residues
42evidence items
0mapped to a state
0states
6techniques
4sources
0conflicts
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | xray | cryoem | nmr_structure | nmr_shifts | force_spectroscopy | af_prediction | Total |
|---|---|---|---|---|---|---|---|---|
| No states defined yet. | ||||||||
Unmapped evidence: 42 of 42 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 0 |
| BMRB | 3 | 0 |
| RCSB PDB | 16 | 0 |
| SMASH-BIO | 22 | 0 |
Structure
No state models are exported for this protein yet. Observed states export the representative PDB entry; inferred states need fitted models, not yet built.
States and transitions
No states defined.
| State | Basis | Parent | Description |
|---|
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-3MUD-2 RCSB PDB | xray | resolution: 2.2 Å | in vitro · -173.15 °C · pH 6.5 · ligands EDO, SO4 Structure of the Tropomyosin Overlap Complex from Chicken Smooth Muscle · Tropomyosin alpha-1 chain,Microtubule-associated protein RP/EB family member 1 (chains C,D) | unmapped |
| EV-PDB-6KN8-3 RCSB PDB | cryoem | resolution: 4.8 Å | in vitro · ligands ADP Structure of human cardiac thin filament in the calcium bound state · Tropomyosin alpha-1 chain (chains R,S,Y,Z) | unmapped |
| EV-PDB-6KN8-2 RCSB PDB | cryoem | resolution: 4.8 Å | in vitro · ligands ADP Structure of human cardiac thin filament in the calcium bound state · Tropomyosin alpha-1 chain (chains P,Q,W,X) | unmapped |
| EV-PDB-6UT2-2 RCSB PDB | nmr_structure | structure | in vitro · 24.85 °C · pH 6.5 3D structure of the leiomodin/tropomyosin binding interface · Tropomyosin alpha-1 chain chimeric peptide (chains B,C) | unmapped |
| EV-PDB-7UTI-2 RCSB PDB | cryoem | resolution: 4.8 Å | in vitro · ligands ADP, CA, MG ALTERNATIVE MODELING OF TROPOMYOSIN IN HUMAN CARDIAC THIN FILAMENT IN THE CALCIUM BOUND STATE · Tropomyosin alpha-1 chain (chains W,b,e,f,g,h,i,j) | unmapped |
| EV-PDB-7UTL-2 RCSB PDB | cryoem | resolution: 6.6 Å | in vitro · ligands ADP, MG ALTERNATIVE MODELING OF TROPOMYOSIN IN HUMAN CARDIAC THIN FILAMENT IN THE CALCIUM FREE STATE · Tropomyosin alpha-1 chain (chains W,Z,a,b,g,h,i,j) | unmapped |
| EV-PDB-8EFH-3 RCSB PDB | cryoem | resolution: 3.3 Å | in vitro · ligands ADP, MG Helical reconstruction of the human cardiac actin-tropomyosin-myosin complex in complex with ADP-Mg2+ · Tropomyosin alpha-1 chain (chains O,P) | unmapped |
| EV-PDB-8EFI-3 RCSB PDB | cryoem | resolution: 3.4 Å | in vitro · ligands ADP, MG Helical reconstruction of the human cardiac actin-tropomyosin-myosin complex in the rigor form · Tropomyosin alpha-1 chain (chains O,P) | unmapped |
| EV-PDB-8ENC-3 RCSB PDB | cryoem | resolution: 3.6 Å | in vitro · ligands ADP, MG Helical reconstruction of the human cardiac actin-tropomyosin-myosin loop 4 7G mutant complex · Tropomyosin alpha-1 chain (chains O,P) | unmapped |
| EV-PDB-8ZB7-3 RCSB PDB | cryoem | resolution: 3.19 Å | in vitro · ligands ADP Human left ventricle ATM complex · Tropomyosin alpha-1 chain (chains L,N,O,P) | unmapped |
| EV-PDB-9ZBL-2 RCSB PDB | cryoem | resolution: 2.79 Å | in vitro · ligands ADP, MG Helical Reconstruction of the Human Cardiac F-Actin-Tropomyosin Complex · Tropomyosin alpha-1 chain (chains M,N,O,P) | unmapped |
| EV-PDB-9ZBP-2 RCSB PDB | cryoem | resolution: 3.12 Å | in vitro · ligands ADP, MG Helical Reconstruction of the Complex of Pseudo-Acetylated Human Cardiac Actin (K326/328Q) and Tropomyosin · Tropomyosin alpha-1 chain (chains M,N,O,P) | unmapped |
| EV-PDB-6X5Z-2 RCSB PDB | cryoem | resolution: 4.24 Å | in vitro · ligands ADP, MG Bovine Cardiac Myosin in Complex with Chicken Skeletal Actin and Human Cardiac Tropomyosin in the Rigor State · Tropomyosin alpha-1 chain (chains O,P) | unmapped |
| EV-PDB-6KN7-3 RCSB PDB | cryoem | resolution: 6.6 Å | in vitro · ligands ADP Structure of human cardiac thin filament in the calcium free state · Tropomyosin alpha-1 chain (chains R,S,Y,Z) | unmapped |
| EV-PDB-6KN7-2 RCSB PDB | cryoem | resolution: 6.6 Å | in vitro · ligands ADP Structure of human cardiac thin filament in the calcium free state · Tropomyosin alpha-1 chain (chains P,Q,W,X) | unmapped |
| EV-PDB-5KHT-1 RCSB PDB | xray | resolution: 1.4964 Å | in vitro · -173.15 °C · pH 7.5 · ligands PEG Crystal structure of the N-terminal fragment of tropomyosin isoform Tpm1.1 at 1.5 A resolution · Tropomyosin alpha-1 chain,General control protein GCN4 (chains A,B,C,D) | unmapped |
| EV-BMRB-30681 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 6.5 3D structure of the leiomodin/tropomyosin binding interface · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-BMRB-5610 BMRB | nmr_shifts | chemical_shift | in vitro · -90.15 °C · pH 6.5 The Structure of the Carboxyl Terminus of Striated alpha-Tropomyosin in Solution Reveals an Unusual Parallel Arrangement of Interacting alpha-Helices · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts, coupling constants | unmapped |
| EV-BMRB-7080 BMRB | nmr_shifts | chemical_shift | in vitro · 9.85 °C Complex of TM1a(1-14)Zip with TM9a(251-284): a model for the polymerization domain ("overlap region")of tropomyosin · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-AFDB-AF-P09493-F1 AlphaFold DB | af_prediction | plddt: 91.62 pLDDT | in silico AlphaFold DB prediction AF-P09493-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-284 | unmapped |
| EV-SMASH-SMFS-000564 SMASH-BIO | force_spectroscopy | study_record | in vitro Probing protein-protein interactions with drag flow: A case study of F-actin and tropomyosin · Troponin/tropomyosin regulatory complex; force spectroscopy unspecified; experiment class: force_generation_motor, rheology_cell | unmapped |
| EV-SMASH-SMFS-000565 SMASH-BIO | force_spectroscopy | study_record | in vitro Probing protein-protein interactions with drag flow: a case study of F-actin and tropomyosin. · Troponin/tropomyosin regulatory complex; force spectroscopy unspecified; experiment class: force_generation_motor, rheology_cell | unmapped |
| EV-SMASH-SMFS-000784 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.3 Functional and Structural Properties of Cytoplasmic Tropomyosin Isoforms Tpm1.8 and Tpm1.9. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction, receptor_ligand_adhesion, rheology_cell | unmapped |
| EV-SMASH-SMFS-001236 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 8 Micro-mechanical response and power-law exponents from the longitudinal fluctuations of F-actin solutions. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor, force_generation_polymerization, rheology_cell | unmapped |
| EV-SMASH-SMFS-001310 SMASH-BIO | force_spectroscopy | study_record | in vitro Single Molecule Mechanics and Kinetics of Cardiac Myosin Interacting with Regulated Thin Filaments · Troponin/tropomyosin regulatory complex; force spectroscopy unspecified; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-002655 SMASH-BIO | force_spectroscopy | study_record | in vitro Cardiomyopathy-associated mutations in tropomyosin differently affect actin-myosin interaction at single-molecule and ensemble levels. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-002737 SMASH-BIO | force_spectroscopy | study_record | in vitro Functional significance of HCM mutants of tropomyosin, V95A and D175N, studied with in vitro motility assays. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-003095 SMASH-BIO | force_spectroscopy | study_record | in vitro Estimation of actomyosin active force maintained by tropomyosin and troponin complex under vertical forces in the in vitro motility assay system. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor, rheology_cell | unmapped |
| EV-SMASH-SMFS-003447 SMASH-BIO | force_spectroscopy | study_record | in vitro Cooperativity of myosin interaction with thin filaments is enhanced by stabilizing substitutions in tropomyosin. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-003868 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 Direct observation of the actin filament by tip-scan atomic force microscopy. · Troponin/tropomyosin regulatory complex; afm smfs; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-003979 SMASH-BIO | force_spectroscopy | study_record | in vitro Muscle Fatigue from the Perspective of a Single Crossbridge. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-004382 SMASH-BIO | force_spectroscopy | study_record | in vitro Stabilizing the central part of tropomyosin increases the bending stiffness of the thin filament. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-004735 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 The role of caldesmon and its phosphorylation by ERK on the binding force of unphosphorylated myosin to actin. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-005309 SMASH-BIO | force_spectroscopy | study_record | in vitro Recent insights into muscle fatigue at the cross-bridge level. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-005310 SMASH-BIO | force_spectroscopy | study_record | in vitro Recent insights into the molecular basis of muscular fatigue. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-005509 SMASH-BIO | force_spectroscopy | study_record | in vitro Force spectroscopy reveals multiple "closed states" of the muscle thin filament. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-005812 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 Human actin mutations associated with hypertrophic and dilated cardiomyopathies demonstrate distinct thin filament regulatory properties in vitro. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-005993 SMASH-BIO | force_spectroscopy | study_record | in vitro Antibody-antigen exchange equilibria in a field of an external force: design of reagentless biosensors. · Troponin/tropomyosin regulatory complex; magnetic tweezers; experiment class: receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-006143 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 Phosphorylation of tropomyosin extends cooperative binding of myosin beyond a single regulatory unit. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-006915 SMASH-BIO | force_spectroscopy | study_record | in vitro Temperature change does not affect force between regulated actin filaments and heavy meromyosin in single-molecule experiments. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-007066-r0 SMASH-BIO | force_spectroscopy | step_size: 5 nm | in vitro Single-myosin crossbridge interactions with actin filaments regulated by troponin-tropomyosin. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor | unmapped |
| EV-SMASH-SMFS-007668 SMASH-BIO | force_spectroscopy | study_record | in vitro In vitro motility of immunoadsorbed brain myosin-V using a Limulus acrosomal process and optical tweezer-based assay. · Troponin/tropomyosin regulatory complex; optical tweezers; experiment class: force_generation_motor, receptor_ligand_adhesion | unmapped |
Conflicts
No conflicts found among comparable evidence.
42 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.