Lactose operon repressor
UniProt P03023 · gene lacI · Escherichia coli (strain K12) · 360 residues
49evidence items
15mapped to a state
2states
5techniques
4sources
0conflicts
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | xray | nmr_structure | nmr_shifts | force_spectroscopy | af_prediction | Total |
|---|---|---|---|---|---|---|---|
| inducer-bound (IPTG) LacI-IPTG | observed (one method) | proc 3 | · | · | · | · | 3 |
| operator DNA-bound LacI-operator | observed | proc 3 | proc 6 | proc 3 | · | · | 12 |
Unmapped evidence: 34 of 49 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 0 |
| BMRB | 14 | 3 |
| RCSB PDB | 25 | 12 |
| SMASH-BIO | 9 | 0 |
Curated rules applied
laci-iptg: 2 itemslaci-operator-title: 11 items
Structure
Choose a state. Each model is badged observed or inferred; assumptions are one click away.
States and transitions
| State | Basis | Parent | Description |
|---|---|---|---|
| inducer-bound (IPTG) LacI-IPTG | observed | — | Repressor bound to the gratuitous inducer IPTG, as in 2P9H. |
| operator DNA-bound LacI-operator | observed | — | Repressor dimer bound to operator DNA with the anti-inducer ONPF, as in 1EFA. |
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-1EFA-2 RCSB PDB | xray | resolution: 2.6 Å | in vitro · -173.15 °C · pH 7.5 · ligands NPF CRYSTAL STRUCTURE OF THE LAC REPRESSOR DIMER BOUND TO OPERATOR AND THE ANTI-INDUCER ONPF · LAC REPRESSOR (chains A,B,C) | operator DNA-bound · curator:draft (unreviewed) · high Repressor bound to operator DNA with ONPF; representative for the operator-bound state. |
| EV-PDB-8GOH-1 RCSB PDB | xray | resolution: 1.1 Å | in vitro · -173.15 °C · ligands 1PE Homotetramic Antiparallel Coiled-Coil of 23-residues LacI C-terminal tetramerization helix · LacI C-terminal tetramerization helix (chains A,B,C,D,E,F,G,H) | unmapped |
| EV-PDB-4RZT-1 RCSB PDB | xray | resolution: 3.1 Å | in vitro · -173.15 °C · pH 7.4 · ligands branched: 4-chloro-4-deoxy-alpha-D-galactopyranose-(1-2)-1,6-dichloro-1,6-dideoxy-beta-D-fructofuranose Lac repressor engineered to bind sucralose, sucralose-bound tetramer · Lac repressor (chains A,B,C,D) | unmapped |
| EV-PDB-4RZS-1 RCSB PDB | xray | resolution: 2.71 Å | in vitro · -173.15 °C · pH 7.4 · ligands GOL Lac repressor engineered to bind sucralose, unliganded tetramer · Lac repressor (chains A,B,C,D) | unmapped |
| EV-PDB-3EDC-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · -173.15 °C · pH 7.5 · ligands HEZ Crystal Structure of a 1.6-hexanediol Bound Tetrameric Form of Escherichia coli Lac-repressor Refined to 2.1 Resolution · Lactose operon repressor (chains A,B,C,D) | unmapped |
| EV-PDB-2PE5-2 RCSB PDB | xray | resolution: 3.5 Å | in vitro · -173.15 °C · pH 7.5 · ligands 145 Crystal Structure of the Lac Repressor bound to ONPG in repressed state · Lactose operon repressor (chains A,B,C) | unmapped |
| EV-PDB-2PAF-1 RCSB PDB | xray | resolution: 3.5 Å | in vitro · -173.15 °C · pH 5.6 · ligands NPF Crystal Structure of the Lactose Repressor bound to anti-inducer ONPF in induced state · Lactose operon repressor (chains A,B) | unmapped |
| EV-PDB-2P9H-1 RCSB PDB | xray | resolution: 2 Å | in vitro · -173.15 °C · pH 5.6 · ligands IPT High resolution structure of the Lactose Repressor bound to IPTG · Lactose operon repressor (chains A,B) | inducer-bound (IPTG) · curator:draft (unreviewed) · high Repressor bound to IPTG; representative for the inducer-bound state. |
| EV-PDB-2KEK-1 RCSB PDB | nmr_structure | structure | in vitro · 41.85 °C · pH 6 Solution structure of a dimer of LAC repressor DNA-binding domain complexed to its natural operator O3 · Lactose operon repressor (chains A,B) | operator DNA-bound · rule:laci-operator-title · medium Title names operator DNA and no inducer is bound. |
| EV-PDB-2KEJ-1 RCSB PDB | nmr_structure | structure | in vitro · 41.85 °C · pH 6 Solution structure of a dimer of LAC repressor DNA-binding domain complexed to its natural operator O2 · Lactose operon repressor (chains A,B) | operator DNA-bound · rule:laci-operator-title · medium Title names operator DNA and no inducer is bound. |
| EV-PDB-2KEI-1 RCSB PDB | nmr_structure | structure | in vitro · 41.85 °C · pH 6 Refined Solution Structure of a Dimer of LAC repressor DNA-Binding domain complexed to its natural operator O1 · Lactose operon repressor (chains A,B) | operator DNA-bound · rule:laci-operator-title · medium Title names operator DNA and no inducer is bound. |
| EV-PDB-2BJC-1 RCSB PDB | nmr_structure | structure | in vitro · 41.85 °C · pH 6 NMR structure of a protein-DNA complex of an altered specificity mutant of the lac repressor headpiece that mimics the gal repressor · LACTOSE OPERON REPRESSOR (chains A,B) | unmapped |
| EV-PDB-1TLF-1 RCSB PDB | xray | resolution: 2.6 Å | in vitro · ligands EMC, IPT UNPRECEDENTED QUATERNARY STRUCTURE OF E. COLI LAC REPRESSOR CORE TETRAMER: IMPLICATIONS FOR DNA LOOPING · LAC REPRESSOR (chains A,B,C,D) | inducer-bound (IPTG) · rule:laci-iptg · high IPTG (IPT) bound. |
| EV-PDB-1OSL-2 RCSB PDB | nmr_structure | structure | in vitro · 26.85 °C · pH 5.8 Solution structure of a dimeric lactose DNA-binding domain complexed to a nonspecific DNA sequence · Lactose operon repressor (chains A,B) | unmapped |
| EV-PDB-1LQC-1 RCSB PDB | nmr_structure | structure | in vitro LAC REPRESSOR HEADPIECE (RESIDUES 1-56), NMR, 32 STRUCTURES · LAC REPRESSOR (chains A) | unmapped |
| EV-PDB-1LCD-3 RCSB PDB | nmr_structure | structure | in vitro · ligands NA STRUCTURE OF THE COMPLEX OF LAC REPRESSOR HEADPIECE AND AN 11 BASE-PAIR HALF-OPERATOR DETERMINED BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY AND RESTRAINED MOLECULAR DYNAMICS · Lac Repressor (chains A) | operator DNA-bound · rule:laci-operator-title · medium Title names operator DNA and no inducer is bound. |
| EV-PDB-1LCC-3 RCSB PDB | nmr_structure | structure | in vitro · ligands NA STRUCTURE OF THE COMPLEX OF LAC REPRESSOR HEADPIECE AND AN 11 BASE-PAIR HALF-OPERATOR DETERMINED BY NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY AND RESTRAINED MOLECULAR DYNAMICS · Lac Repressor (chains A) | operator DNA-bound · rule:laci-operator-title · medium Title names operator DNA and no inducer is bound. |
| EV-PDB-1LBI-1 RCSB PDB | xray | resolution: 2.7 Å | in vitro LAC REPRESSOR · LAC REPRESSOR (chains A,B,C,D) | unmapped |
| EV-PDB-1LBH-1 RCSB PDB | xray | resolution: 3.2 Å | in vitro · ligands IPT INTACT LACTOSE OPERON REPRESSOR WITH GRATUITOUS INDUCER IPTG · INTACT LACTOSE OPERON REPRESSOR WITH GRATUITOUS INDUCER IPTG (chains A,B,C,D) | inducer-bound (IPTG) · rule:laci-iptg · high IPTG (IPT) bound. |
| EV-PDB-1LBG-2 RCSB PDB | xray | resolution: 4.8 Å | in vitro LACTOSE OPERON REPRESSOR BOUND TO 21-BASE PAIR SYMMETRIC OPERATOR DNA, ALPHA CARBONS ONLY · PROTEIN (LACTOSE OPERON REPRESSOR) (chains A,B,C,D) | operator DNA-bound · rule:laci-operator-title · medium Title names operator DNA and no inducer is bound. |
| EV-PDB-1L1M-3 RCSB PDB | nmr_structure | structure | in vitro · 41.85 °C · pH 6 SOLUTION STRUCTURE OF A DIMER OF LAC REPRESSOR DNA-BINDING DOMAIN COMPLEXED TO ITS NATURAL OPERATOR O1 · Lactose operon repressor (chains A,B) | operator DNA-bound · rule:laci-operator-title · medium Title names operator DNA and no inducer is bound. |
| EV-PDB-1JYF-1 RCSB PDB | xray | resolution: 3 Å | in vitro · -173.15 °C · pH 5.6 · ligands GOL Structure of the Dimeric Lac Repressor with an 11-residue C-terminal Deletion. · Lactose Operon Repressor (chains A) | unmapped |
| EV-PDB-1JYE-1 RCSB PDB | xray | resolution: 1.7 Å | in vitro · -173.15 °C · pH 5.6 · ligands GOL Structure of a Dimeric Lac Repressor with C-terminal Deletion and K84L Substitution · Lactose Operon Repressor (chains A) | unmapped |
| EV-PDB-1JWL-3 RCSB PDB | xray | resolution: 4 Å | in vitro · 21.85 °C · pH 7.5 · ligands NPF Structure of the Dimeric lac Repressor/Operator O1/ONPF Complex · Lactose Operon Repressor (chains A,B,C) | operator DNA-bound · rule:laci-operator-title · medium Title names operator DNA and no inducer is bound. |
| EV-PDB-1CJG-2 RCSB PDB | nmr_structure | structure | in vitro · 41.85 °C · pH 6.1 NMR STRUCTURE OF LAC REPRESSOR HP62-DNA COMPLEX · PROTEIN (LAC REPRESSOR) (chains A,B) | unmapped |
| EV-BMRB-1066 BMRB | nmr_shifts | chemical_shift | in vitro · 22.85 °C · pH 7.8 High-Resolution Nuclear Magnetic Resonance Studies of the Lac Repressor. 2. Partial Analysis of the Aliphatic Region of the Lac Repressor Headpiece Spectrum · BMRB data: 1H chemical shifts | unmapped |
| EV-BMRB-127 BMRB | nmr_shifts | chemical_shift | in vitro · 17.85 °C · pH 4 Sequence-specific resonance assignments in the 1H nuclear-magnetic-resonance spectrum of the Lac repressor DNA-binding domain 1-51 from Escherichia coli by two-dimensional spectroscopy · BMRB data: 1H chemical shifts | unmapped |
| EV-BMRB-1494 BMRB | nmr_shifts | chemical_shift | in vitro · 26.85 °C · pH 6 Assignment of the 1H-NMR spectrum of a lac repressor headpiece-operator complex in H2O and identification of NOEs · BMRB data: 1H chemical shifts | operator DNA-bound · rule:laci-operator-title · medium Title names operator DNA and no inducer is bound. |
| EV-BMRB-1552 BMRB | nmr_shifts | chemical_shift | in vitro · 22.85 °C · pH 1 pH Dependence of the High-Resolution Proton Nuclear Magnetic Resonance Spectrum of the lac Repressor Headpiece · BMRB data: 1H chemical shifts | unmapped |
| EV-BMRB-2956 BMRB | nmr_shifts | chemical_shift | in vitro · 21.85 °C · pH 6 Importance of Protein and DNA Phosphate Ester Backbone Flexibility in Protein-DNA Recognition · BMRB data: 1H chemical shifts | unmapped |
| EV-BMRB-32 BMRB | nmr_shifts | chemical_shift | in vitro · 17.85 °C · pH 6.85 Sequence-specific resonance assignments in the 1H nuclear-magnetic-resonance spectrum of the Lac repressor DNA-binding domain 1-51 from Escherichia coli by two-dimensional spectroscopy · BMRB data: 1H chemical shifts | unmapped |
| EV-BMRB-4813 BMRB | nmr_shifts | chemical_shift | in vitro · 41.85 °C · pH 6.1 NMR structure of lac repressor HP62-DNA complex · BMRB data: 1H chemical shifts, 13C chemical shifts, 15N chemical shifts | unmapped |
| EV-BMRB-5345 BMRB | nmr_shifts | chemical_shift | in vitro · 41.85 °C · pH 6 Assignment of lac repressor headpiece complexed of its natural operator · BMRB data: 13C chemical shifts, 1H chemical shifts, 15N chemical shifts | operator DNA-bound · rule:laci-operator-title · medium Title names operator DNA and no inducer is bound. |
| EV-BMRB-5791 BMRB | nmr_shifts | chemical_shift | in vitro · 26.85 °C · pH 5.8 Solution structure of a dimeric lactose DNA-binding domain complexed to a nonspecific DNA sequence · BMRB data: 13C chemical shifts, 1H chemical shifts, 15N chemical shifts | unmapped |
| EV-BMRB-661 BMRB | nmr_shifts | chemical_shift | in vitro · 22.85 °C · pH 7.8 A Folded Structure for the Lac-Repressor Headpiece · BMRB data: 1H chemical shifts | unmapped |
| EV-BMRB-736 BMRB | nmr_shifts | chemical_shift | in vitro · 26.85 °C · pH 7 Complex of lac Repressor Headpiece with a 14 Base-pair lac Operator Fragment Studied by Two-dimensional Nuclear Magnetic Resonance · BMRB data: 1H chemical shifts | operator DNA-bound · rule:laci-operator-title · medium Title names operator DNA and no inducer is bound. |
| EV-BMRB-848 BMRB | nmr_shifts | chemical_shift | in vitro · 22.85 °C · pH 7.8 High-Resolution Nuclear Magnetic Resonance Studies of the Lac Repressor. 1. Assignments of Tyrosine Resonances in the N-Terminal Headpiece · BMRB data: 1H chemical shifts | unmapped |
| EV-BMRB-849 BMRB | nmr_shifts | chemical_shift | in vitro · 22.85 °C · pH 7.8 High-Resolution Nuclear Magnetic Resonance Studies of the Lac Repressor. 2. Partial Analysis of the Aliphatic Region of the Lac Repressor Headpiece Spectrum · BMRB data: 1H chemical shifts | unmapped |
| EV-BMRB-96 BMRB | nmr_shifts | chemical_shift | in vitro · 17.85 °C · pH 4.95 Sequence-specific resonance assignments in the 1H nuclear-magnetic-resonance spectrum of the Lac repressor DNA-binding domain 1-51 from Escherichia coli by two-dimensional spectroscopy · BMRB data: 1H chemical shifts | unmapped |
| EV-AFDB-AF-P03023-F1 AlphaFold DB | af_prediction | plddt: 93.69 pLDDT | in silico AlphaFold DB prediction AF-P03023-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-360 | unmapped |
| EV-SMASH-SMFS-000773 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 8 Force and the α-C-terminal domains bias RNA polymerase recycling. · Lac repressor (LacI); magnetic tweezers; experiment class: translation_transcription_replication, force_generation_motor, nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-001162 SMASH-BIO | force_spectroscopy | study_record | in vitro Force and the α -C-terminal domains bias RNA polymerase recycling · Lac repressor; magnetic tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-002677 SMASH-BIO | force_spectroscopy | study_record | in vitro Data on the target search by a single protein on DNA measured with ultrafast force-clamp spectroscopy. · Lac repressor; optical tweezers; experiment class: nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-003247 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 Protein-mediated looping of DNA under tension requires supercoiling. · Lac repressor; magnetic tweezers; experiment class: chromatin_mechanics, nucleic_acid_mechanics, nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-003248 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 Protein-mediated loops in supercoiled DNA create large topological domains. · Lac repressor; magnetic tweezers; experiment class: chromatin_mechanics, nucleic_acid_mechanics, nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-003308 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 Sliding of a single lac repressor protein along DNA is tuned by DNA sequence and molecular switching. · Lac repressor; optical tweezers; experiment class: nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-004491 SMASH-BIO | force_spectroscopy | study_record | in vitro Combining single-molecule manipulation and imaging for the study of protein-DNA interactions. · Lac repressor; optical tweezers; experiment class: force_generation_motor, nucleic_acid_protein_interaction | unmapped |
| EV-SMASH-SMFS-006459 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7 Single-molecule manipulation reveals supercoiling-dependent modulation of lac repressor-mediated DNA looping. · Lac repressor; force spectroscopy unspecified; experiment class: nucleic_acid_mechanics, nucleic_acid_protein_interaction, translation_transcription_replication | unmapped |
| EV-SMASH-SMFS-006960 SMASH-BIO | force_spectroscopy | study_record | in vitro Detection of high-affinity and sliding clamp modes for MSH2-MSH6 by single-molecule unzipping force analysis. · Lac repressor; force spectroscopy unspecified; experiment class: nucleic_acid_protein_interaction, unfolding_protein | unmapped |
Conflicts
No conflicts found among comparable evidence.
49 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.
Downloads
- Protein bundle (JSON)
- LacI-IPTG-cif (mmCIF, observed)
- LacI-IPTG-pdb (PDB, observed)
- LacI-operator-cif (mmCIF, observed)
- LacI-operator-pdb (PDB, observed)
- Curation file (YAML)