ATP-dependent molecular chaperone HSP82
UniProt P02829 · gene HSP82 · Saccharomyces cerevisiae (strain ATCC 204508 / S288c) · 709 residues
56evidence items
2mapped to a state
2states
6techniques
5sources
0conflicts
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | xray | nmr_structure | nmr_shifts | integrative | af_prediction | md | Total |
|---|---|---|---|---|---|---|---|---|
| closed, nucleotide-bound chaperone complex Hsp90-closed | observed (one method) | proc 1 | · | · | · | · | · | 1 |
| N-terminal domain, ligand-free Hsp90-NTD-apo | observed (one method) | proc 1 | · | · | · | · | · | 1 |
Unmapped evidence: 54 of 56 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 0 |
| BMRB | 2 | 0 |
| DisProt | 1 | 0 |
| MDDB | 2 | 0 |
| RCSB PDB | 50 | 2 |
Structure
Choose a state. Each model is badged observed or inferred; assumptions are one click away.
States and transitions
| State | Basis | Parent | Description |
|---|---|---|---|
| closed, nucleotide-bound chaperone complex Hsp90-closed | observed | — | Closed chaperone complex with Sba1 and a nucleotide, as in 2CG9. Full-length context, complex-level state. |
| N-terminal domain, ligand-free Hsp90-NTD-apo | observed | — | N-terminal domain only, as in 1AH6. Not a full-length conformation. |
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-1A4H-1 RCSB PDB | xray | resolution: 2.5 Å | in vitro · -163.15 °C · pH 5 · ligands GDM STRUCTURE OF THE N-TERMINAL DOMAIN OF THE YEAST HSP90 CHAPERONE IN COMPLEX WITH GELDANAMYCIN · HEAT SHOCK PROTEIN 90 (chains A) | unmapped |
| EV-PDB-1AMW-1 RCSB PDB | xray | resolution: 1.85 Å | in vitro · -163.15 °C · pH 5 · ligands ADP ADP BINDING SITE IN THE HSP90 MOLECULAR CHAPERONE · HEAT SHOCK PROTEIN 90 (chains A) | unmapped |
| EV-PDB-1BGQ-1 RCSB PDB | xray | resolution: 2.5 Å | in vitro · -153.15 °C · pH 5 · ligands RDC RADICICOL BOUND TO THE ATP BINDING SITE OF THE N-TERMINAL DOMAIN OF THE YEAST HSP90 CHAPERONE · HEAT SHOCK PROTEIN 90 (chains A) | unmapped |
| EV-PDB-1HK7-1 RCSB PDB | xray | resolution: 2.5 Å | in vitro · -173.15 °C · pH 7.5 · ligands CD, MG Middle Domain of HSP90 · HEAT SHOCK PROTEIN HSP82 (chains A,B) | unmapped |
| EV-PDB-1USV-1 RCSB PDB | xray | resolution: 2.7 Å | in vitro · -173.15 °C · pH 6.5 The Structure of the complex between Aha1 and HSP90 · HEAT SHOCK PROTEIN HSP82 (chains A,C,E,G) | unmapped |
| EV-PDB-1ZW9-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · -161.15 °C · pH 4.6 · ligands H64 Yeast HSP82 in complex with the Novel HSP90 Inhibitor 8-(6-Bromo-benzo[1,3]dioxol-5-ylsulfanyl)-9-(3-isopropylamino-propyl)-adenine · HSP82 (chains A) | unmapped |
| EV-PDB-1ZWH-1 RCSB PDB | xray | resolution: 1.65 Å | in vitro · -173.15 °C · pH 5 · ligands RDE Yeast Hsp82 in complex with the novel Hsp90 inhibitor Radester amine · ATP-dependent molecular chaperone HSP82 (chains A) | unmapped |
| EV-PDB-2AKP-1 RCSB PDB | xray | resolution: 1.94 Å | in vitro · -173.15 °C · pH 6.5 Hsp90 Delta24-N210 mutant · ATP-dependent molecular chaperone HSP82 (chains A,B) | unmapped |
| EV-PDB-2BRC-1 RCSB PDB | xray | resolution: 1.6 Å | in vitro · -173.15 °C · pH 7 · ligands CT5 Structure of a Hsp90 Inhibitor bound to the N-terminus of Yeast Hsp90. · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-2BRE-1 RCSB PDB | xray | resolution: 2 Å | in vitro · -173.15 °C · pH 7 · ligands KJ2 STRUCTURE OF A HSP90 INHIBITOR BOUND TO THE N-TERMINUS OF YEAST HSP90. · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A,B) | unmapped |
| EV-PDB-2CG9-1 RCSB PDB | xray | resolution: 3.1 Å | in vitro · -173.15 °C · pH 7.5 · ligands ATP Crystal structure of an Hsp90-Sba1 closed chaperone complex · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A,B) | closed, nucleotide-bound chaperone complex · curator:draft (unreviewed) · medium Closed conformation of Hsp82 in a complex with Sba1; the state is complex-dependent, so confidence is medium. |
| EV-PDB-2CGE-1 RCSB PDB | xray | resolution: 3 Å | in vitro · -173.15 °C · pH 7.5 Crystal structure of an Hsp90-Sba1 closed chaperone complex · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A,B,D) | unmapped |
| EV-PDB-2FXS-1 RCSB PDB | xray | resolution: 2 Å | in vitro · -173.15 °C · pH 5 · ligands GOL, RDA Yeast HSP82 in complex with the novel HSP90 Inhibitor Radamide · ATP-dependent molecular chaperone HSP82 (chains A) | unmapped |
| EV-PDB-2LSV-2 RCSB PDB | nmr_structure | structure | in vitro · 14.85 °C · pH 7.2 The NMR high resolution structure of yeast Tah1 in complex with the Hsp90 C-terminal tail · ATP-dependent molecular chaperone HSP82 (chains B) | unmapped |
| EV-PDB-2VW5-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · -173.15 °C · ligands BC6, SO4 Structure Of The Hsp90 Inhibitor 7-O-carbamoylpremacbecin Bound To The N- Terminus Of Yeast Hsp90 · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A,B,C,D) | unmapped |
| EV-PDB-2VWC-1 RCSB PDB | xray | resolution: 2.4 Å | in vitro · -173.15 °C · pH 7 · ligands BC2, GOL STRUCTURE OF THE HSP90 INHIBITOR MACBECIN BOUND TO THE N-TERMINUS OF YEAST HSP90. · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-2WEQ-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · -173.15 °C · pH 7 · ligands GDM, GOL Yeast Hsp90 N-terminal domain LI-IV mutant with Geldanamycin · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-2XX4-1 RCSB PDB | xray | resolution: 2.199 Å | in vitro · -173.15 °C · pH 7 · ligands 13I Macrolactone Inhibitor bound to HSP90 N-term · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-2YGA-1 RCSB PDB | xray | resolution: 2.37 Å | in vitro · -173.15 °C · pH 7 · ligands GDM E88G-N92L Mutant of N-Term HSP90 complexed with Geldanamycin · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-2YGE-1 RCSB PDB | xray | resolution: 1.956 Å | in vitro · -173.15 °C · pH 7 · ligands GDM, GOL E88G-N92L Mutant of N-Term HSP90 complexed with Geldanamycin · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-3C0E-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · -173.15 °C · pH 5 Yeast Hsp82 N-terminal domain: effects of mutants 98-99 KS-AA · ATP-dependent molecular chaperone HSP82 (chains A) | unmapped |
| EV-PDB-3C11-1 RCSB PDB | xray | resolution: 1.6 Å | in vitro · -173.15 °C · pH 5 · ligands GDM, GOL Yeast Hsp82 N-terminal domain-Geldanamycin complex: effects of mutants 98-99 KS-AA · ATP-dependent molecular chaperone HSP82 (chains A) | unmapped |
| EV-PDB-4AS9-1 RCSB PDB | xray | resolution: 2.71 Å | in vitro · -173.15 °C · pH 7 · ligands 4QS The structure of modified benzoquinone ansamycins bound to yeast N- terminal Hsp90 · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-4ASA-1 RCSB PDB | xray | resolution: 2.25 Å | in vitro · -173.15 °C · pH 7 · ligands 59C, CL The structure of modified benzoquinone ansamycins bound to yeast N- terminal Hsp90 · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-4ASB-1 RCSB PDB | xray | resolution: 3.08 Å | in vitro · -173.15 °C · pH 7 · ligands 8TO The structure of modified benzoquinone ansamycins bound to yeast N- terminal Hsp90 · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-4ASG-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · -173.15 °C · pH 7 · ligands 814, NI The structure of modified benzoquinone ansamycins bound to yeast N- terminal Hsp90 · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-4CE3-1 RCSB PDB | xray | resolution: 2.31 Å | in vitro · -173.15 °C · pH 7 · ligands L4V Hsp90 N-terminal domain bound to macrolactam analogues of radicicol. · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-5MGX-1 RCSB PDB | xray | resolution: 2.18 Å | in vitro · -173.15 °C The structure of FKBP38 in complex with the MEEVD tetratricopeptide binding-motif of Hsp90 · yeast HSP90 C-terminus (chains A,B,C,D) | unmapped |
| EV-PDB-8OXU-1 RCSB PDB | xray | resolution: 2.94 Å | in vitro · -173.15 °C · pH 7.5 · ligands W5R Crystal Structure of the Hsp90-LA1011 Complex · ATP-dependent molecular chaperone HSP82 (chains A,B,C,D) | unmapped |
| EV-PDB-9AAO-1 RCSB PDB | integrative | structure | in vitro · ligands ADP, MG Yeast Hsp90 open structural ensemble · ATP-dependent molecular chaperone HSP82 (chains A,B) | unmapped |
| EV-PDB-9Q8O-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · -80.15 °C Crystal structure of Hsp82-MD in complex with the CS domain of Sgt1 · ATP-dependent molecular chaperone HSP82 (chains A) | unmapped |
| EV-PDB-4CE2-1 RCSB PDB | xray | resolution: 2.38 Å | in vitro · -173.15 °C · pH 7 · ligands BO5 Hsp90 N-terminal domain bound to macrolactam analogues of radicicol. · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-4CE1-1 RCSB PDB | xray | resolution: 2.01 Å | in vitro · -173.15 °C · pH 7 · ligands 7FK Hsp90 N-terminal domain bound to macrolactam analogues of radicicol. · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-4ASF-1 RCSB PDB | xray | resolution: 2.6 Å | in vitro · -173.15 °C · pH 7 · ligands 62U, NI The structure of modified benzoquinone ansamycins bound to yeast N- terminal Hsp90 · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-3FP2-2 RCSB PDB | xray | resolution: 1.98 Å | in vitro · -73.15 °C · pH 8 · ligands CL, MG Crystal structure of Tom71 complexed with Hsp82 C-terminal fragment · ATP-dependent molecular chaperone HSP82 (chains Q) | unmapped |
| EV-PDB-2YGF-1 RCSB PDB | xray | resolution: 2 Å | in vitro · -173.15 °C · pH 7 · ligands GDM, GOL L89V, L93I and V136M Mutant of N-Term HSP90 complexed with Geldanamycin · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-2XX5-1 RCSB PDB | xray | resolution: 2 Å | in vitro · -173.15 °C · pH 7 · ligands 13N, GOL Macrolactone Inhibitor bound to HSP90 N-term · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-2XX2-1 RCSB PDB | xray | resolution: 1.85 Å | in vitro · -173.15 °C · pH 7 · ligands 13C, GOL Macrolactone Inhibitor bound to HSP90 N-term · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A,B,C,D) | unmapped |
| EV-PDB-2XD6-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · -173.15 °C · pH 7 · ligands GOL, XD6 Hsp90 complexed with a resorcylic acid macrolactone. · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-2WER-1 RCSB PDB | xray | resolution: 1.6 Å | in vitro · -173.15 °C · pH 7 · ligands RDC Yeast Hsp90 N-terminal domain LI-IV mutant with Radicicol · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A,B) | unmapped |
| EV-PDB-2WEP-1 RCSB PDB | xray | resolution: 2 Å | in vitro · -173.15 °C · pH 7 · ligands ADP Yeast Hsp90 N-terminal domain LI-IV mutant with ADP · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-2IWX-1 RCSB PDB | xray | resolution: 1.5 Å | in vitro · -173.15 °C · ligands M1S Analogues of radicicol bound to the ATP-binding site of Hsp90. · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-2IWU-1 RCSB PDB | xray | resolution: 2.8 Å | in vitro · -173.15 °C · ligands NP5 Analogues of radicicol bound to the ATP-binding site of Hsp90 · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-2IWS-1 RCSB PDB | xray | resolution: 2.7 Å | in vitro · -173.15 °C · ligands NP4 Radicicol analogues bound to the ATP site of HSP90 · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-2CGF-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · -173.15 °C · ligands P2N A RADICICOL ANALOGUE BOUND TO THE ATP BINDING SITE OF THE N-TERMINAL DOMAIN OF THE YEAST HSP90 CHAPERONE · ATP-DEPENDENT MOLECULAR CHAPERONE HSP82 (chains A) | unmapped |
| EV-PDB-1USU-1 RCSB PDB | xray | resolution: 2.15 Å | in vitro · -173.15 °C · pH 6.5 · ligands GOL The Structure of the complex between Aha1 and HSP90 · HEAT SHOCK PROTEIN HSP82 (chains A) | unmapped |
| EV-PDB-1US7-1 RCSB PDB | xray | resolution: 2.3 Å | in vitro · -173.15 °C · pH 6 Complex of Hsp90 and P50 · HEAT SHOCK PROTEIN HSP82 (chains A) | unmapped |
| EV-PDB-1AM1-1 RCSB PDB | xray | resolution: 2 Å | in vitro · -163.15 °C · pH 5 · ligands ADP ATP BINDING SITE IN THE HSP90 MOLECULAR CHAPERONE · HEAT SHOCK PROTEIN 90 (chains A) | unmapped |
| EV-PDB-1AH8-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · -173.15 °C · pH 5 · ligands GOL STRUCTURE OF THE ORTHORHOMBIC FORM OF THE N-TERMINAL DOMAIN OF THE YEAST HSP90 CHAPERONE · HEAT SHOCK PROTEIN 90 (chains A,B) | unmapped |
| EV-PDB-1AH6-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · -173.15 °C · pH 5 STRUCTURE OF THE TETRAGONAL FORM OF THE N-TERMINAL DOMAIN OF THE YEAST HSP90 CHAPERONE · HEAT SHOCK PROTEIN 90 (chains A) | N-terminal domain, ligand-free · curator:draft (unreviewed) · high N-terminal domain only, ligand-free; representative for the NTD apo state. |
| EV-BMRB-16279 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 7.5 Backbone 1H, 13C, and 15N Chemical Shift Assignments for the yeast Hsp90 Middle Domain · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-BMRB-5355 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C Backbone resonance assignments of the 25kD N-terminal ATPase domain from the Hsp90 chaperone · BMRB data: 1H chemical shifts, 13C chemical shifts, 15N chemical shifts | unmapped |
| EV-DISPROT-DP04448r002 DisProt | xray | disorder_region: 32 residues | in vitro DisProt DP04448: disorder, residues 678-709 · residues 678-709; evidence: X-ray crystallography-based structural model with missing residue coordinates used in manual assertion | unmapped |
| EV-AFDB-AF-P02829-F1 AlphaFold DB | af_prediction | plddt: 84.94 pLDDT | in silico AlphaFold DB prediction AF-P02829-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-709 | unmapped |
| EV-MDDB-A000Y MDDB | md | trajectory: 10 ns | in silico · 26.85 °C MoDEL 1A4H · start 1A4H; Amber Parm99 | unmapped |
| EV-MDDB-A0031 MDDB | md | trajectory: 10 ns | in silico · 26.85 °C MoDEL 1AH6 · start 1AH6; Amber Parm99 | unmapped |
Conflicts
No conflicts found among comparable evidence.
56 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.
Downloads
- Protein bundle (JSON)
- Hsp90-closed-cif (mmCIF, observed)
- Hsp90-closed-pdb (PDB, observed)
- Hsp90-NTD-apo-cif (mmCIF, observed)
- Hsp90-NTD-apo-pdb (PDB, observed)
- Curation file (YAML)