Spectrin alpha chain, erythrocytic 1
UniProt P02549 · gene SPTA1 · Homo sapiens · 2419 residues
52evidence items
0mapped to a state
0states
6techniques
6sources
0conflicts
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | xray | nmr_structure | nmr_shifts | force_spectroscopy | af_prediction | md | Total |
|---|---|---|---|---|---|---|---|---|
| No states defined yet. | ||||||||
Unmapped evidence: 52 of 52 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 0 |
| BMRB | 1 | 0 |
| DisProt | 1 | 0 |
| MDDB | 1 | 0 |
| RCSB PDB | 3 | 0 |
| SMASH-BIO | 45 | 0 |
Structure
No state models are exported for this protein yet. Observed states export the representative PDB entry; inferred states need fitted models, not yet built.
States and transitions
No states defined.
| State | Basis | Parent | Description |
|---|
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-1OWA-1 RCSB PDB | nmr_structure | structure | in vitro · 19.85 °C · pH 6.5 Solution Structural Studies on Human Erythrocyte Alpha Spectrin N Terminal Tetramerization Domain · Spectrin alpha chain, erythrocyte (chains A) | unmapped |
| EV-PDB-3LBX-1 RCSB PDB | xray | resolution: 2.8 Å | in vitro · -173.15 °C · pH 6.5 Crystal Structure of the Erythrocyte Spectrin Tetramerization Domain Complex · Spectrin alpha chain, erythrocyte (chains A) | unmapped |
| EV-PDB-5J4O-1 RCSB PDB | xray | resolution: 1.54 Å | in vitro · -173.15 °C · pH 6.5 · ligands EDO, PG4 Structure of human erythrocytic Spectrin alpha chain repeats 16-17 · Spectrin alpha chain, erythrocytic 1 (chains A) | unmapped |
| EV-BMRB-26773 BMRB | nmr_shifts | chemical_shift | in vitro · 39.85 °C · pH 6.5 Chemical shift assignments of Spectrin repeat a17 · BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts | unmapped |
| EV-DISPROT-DP01700r001 DisProt | nmr_shifts | disorder_region: 20 residues | in vitro DisProt DP01700: disorder, residues 1-20 · residues 1-20; evidence: nuclear magnetic resonance spectroscopy evidence used in manual assertion | unmapped |
| EV-AFDB-AF-P02549-F1 AlphaFold DB | af_prediction | plddt: 76.38 pLDDT | in silico AlphaFold DB prediction AF-P02549-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-2419 | unmapped |
| EV-MDDB-A00TE MDDB | md | trajectory: 10 ns | in silico · 26.85 °C MoDEL 1OWA · start 1OWA; Amber Parm99 | unmapped |
| EV-SMASH-SMFS-000016 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 Active Force Dynamics in Red Blood Cells Under Non-Invasive Optical Tweezers. · Spectrin (repeats); optical tweezers; experiment class: force_generation_motor, membrane_mechanics, receptor_ligand_adhesion, rheology_cell | unmapped |
| EV-SMASH-SMFS-000112 SMASH-BIO | force_spectroscopy | study_record | in vitro Force-dependent structural dynamics of the giant nesprin-2. · Spectrin (repeats); magnetic tweezers; experiment class: rheology_cell, unfolding_protein | unmapped |
| EV-SMASH-SMFS-000257 SMASH-BIO | force_spectroscopy | study_record | in vitro Resolving Heterogeneous Mechanical Domains via Physics-Aware Deep Clustering of Single-Molecule Force Spectroscopy Data · Spectrin (repeats); afm smfs; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-000373 SMASH-BIO | force_spectroscopy | study_record | in vitro Actin instability alters red blood cell mechanics and Piezo1 channel activity. · Spectrin (repeats); optical tweezers; experiment class: membrane_mechanics, rheology_cell | unmapped |
| EV-SMASH-SMFS-000506 SMASH-BIO | force_spectroscopy | study_record | in vitro Mechanical Responses of the Giant Nesprin-2 · Spectrin (repeats); magnetic tweezers; experiment class: rheology_cell, unfolding_protein | unmapped |
| EV-SMASH-SMFS-000599 SMASH-BIO | force_spectroscopy | study_record | in vitro Salt-bridge mediated cooperativity and mechanical stabilization of tandem spectrin repeats. · Spectrin (repeats); force spectroscopy unspecified; experiment class: nucleic_acid_protein_interaction, rheology_cell, unfolding_protein | unmapped |
| EV-SMASH-SMFS-001123 SMASH-BIO | force_spectroscopy | study_record | in vitro Dynamics of the axon plasma membrane skeleton. · Spectrin (repeats); force spectroscopy unspecified; experiment class: rheology_cell | unmapped |
| EV-SMASH-SMFS-001820 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.5 An asymmetric mechanical code ciphers curvature-dependent proprioceptor activity. · Spectrin (repeats); optical tweezers; experiment class: force_generation_motor, rheology_cell | unmapped |
| EV-SMASH-SMFS-002001 SMASH-BIO | force_spectroscopy | study_record | in vitro Mechanical Stretch Inhibition Sensitizes Proprioceptors to Compressive Stresses · Spectrin (repeats); force spectroscopy unspecified; experiment class: force_generation_motor, rheology_cell | unmapped |
| EV-SMASH-SMFS-002292 SMASH-BIO | force_spectroscopy | study_record | in vitro Deformation behaviour of stomatocyte, discocyte and echinocyte red blood cell morphologies during optical tweezers stretching. · Spectrin (repeats); optical tweezers; experiment class: rheology_cell | unmapped |
| EV-SMASH-SMFS-002438 SMASH-BIO | force_spectroscopy | study_record | in vitro Modulating mechanical stability of heterodimerization between engineered orthogonal helical domains. · Spectrin (repeats); afm smfs; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-002791-r0 SMASH-BIO | force_spectroscopy | loading_rate: 1 pN/s | in vitro · pH 8.5 Mechanical unfolding of spectrin reveals a super-exponential dependence of unfolding rate on force. · Spectrin (repeats); magnetic tweezers; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-003087 SMASH-BIO | force_spectroscopy | study_record | in vitro Dystrophin As a Molecular Shock Absorber. · Spectrin (repeats); magnetic tweezers; experiment class: rheology_cell, unfolding_protein | unmapped |
| EV-SMASH-SMFS-003382 SMASH-BIO | force_spectroscopy | study_record | in vitro α-Helix Unwinding as Force Buffer in Spectrins. · Spectrin (repeats); afm smfs; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-003741 SMASH-BIO | force_spectroscopy | study_record | in vitro Study of in vitro RBCs membrane elasticity with AOD scanning optical tweezers. · Spectrin (repeats); optical tweezers; experiment class: rheology_cell | unmapped |
| EV-SMASH-SMFS-004379-r0 SMASH-BIO | force_spectroscopy | unbinding_force: 30 pN | in vitro Spectrin-ankyrin interaction mechanics: A key force balance factor in the red blood cell membrane skeleton. · Spectrin (repeats); afm smfs; experiment class: receptor_ligand_adhesion, rheology_cell | unmapped |
| EV-SMASH-SMFS-004401 SMASH-BIO | force_spectroscopy | study_record | in vitro The First Step of Amyloidogenic Aggregation. · Spectrin (repeats); afm smfs; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-004499 SMASH-BIO | force_spectroscopy | study_record | in vitro Computational biorheology of human blood flow in health and disease. · Spectrin (repeats); optical tweezers; experiment class: rheology_cell | unmapped |
| EV-SMASH-SMFS-004605 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 6 Mechanical control of the sense of touch by β-spectrin. · Spectrin (repeats); afm smfs; experiment class: receptor_ligand_adhesion, rheology_cell | unmapped |
| EV-SMASH-SMFS-004870 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7.4 Force spectroscopy measurements show that cortical neurons exposed to excitotoxic agonists stiffen before showing evidence of bleb damage. · Spectrin (repeats); afm smfs; experiment class: force_generation_motor, rheology_cell | unmapped |
| EV-SMASH-SMFS-005087 SMASH-BIO | force_spectroscopy | study_record | in vitro [Deformability of the erythrocytes membrane in rats of different age in hypoxia]. · Spectrin (repeats); optical tweezers; experiment class: membrane_mechanics, rheology_cell | unmapped |
| EV-SMASH-SMFS-005201 SMASH-BIO | force_spectroscopy | study_record | in vitro Forced extension of delipidated red blood cell cytoskeleton with little indication of spectrin unfolding. · Spectrin (repeats); force spectroscopy unspecified; experiment class: receptor_ligand_adhesion, rheology_cell | unmapped |
| EV-SMASH-SMFS-005210 SMASH-BIO | force_spectroscopy | study_record | in vitro How malaria parasites reduce the deformability of infected red blood cells. · Spectrin (repeats); optical tweezers; experiment class: rheology_cell | unmapped |
| EV-SMASH-SMFS-005244-r0 SMASH-BIO | force_spectroscopy | unfolding_force: 30 pN | in vitro Mechanical anisotropy of ankyrin repeats. · Spectrin (repeats); afm smfs; experiment class: rheology_cell, unfolding_protein | unmapped |
| EV-SMASH-SMFS-005475 SMASH-BIO | force_spectroscopy | study_record | in vitro Differential mechanical stability of filamin A rod segments. · Spectrin (repeats); magnetic tweezers; experiment class: force_generation_polymerization, unfolding_protein | unmapped |
| EV-SMASH-SMFS-005860 SMASH-BIO | force_spectroscopy | study_record | in vitro Multiscale simulation of erythrocyte membranes. · Spectrin (repeats); optical tweezers; experiment class: membrane_mechanics, rheology_cell, unfolding_protein | unmapped |
| EV-SMASH-SMFS-005938 SMASH-BIO | force_spectroscopy | study_record | in vitro Systematic coarse-graining of spectrin-level red blood cell models. · Spectrin (repeats); optical tweezers; experiment class: rheology_cell | unmapped |
| EV-SMASH-SMFS-005987 SMASH-BIO | force_spectroscopy | study_record | in vitro ATP-dependent mechanics of red blood cells. · Spectrin (repeats); optical tweezers; experiment class: rheology_cell | unmapped |
| EV-SMASH-SMFS-006563 SMASH-BIO | force_spectroscopy | study_record | in vitro Effect of plasmodial RESA protein on deformability of human red blood cells harboring Plasmodium falciparum. · Spectrin (repeats); optical tweezers; experiment class: receptor_ligand_adhesion, rheology_cell | unmapped |
| EV-SMASH-SMFS-006802 SMASH-BIO | force_spectroscopy | study_record | in vitro Extending a spectrin repeat unit. I: linear force-extension response. · Spectrin (repeats); force spectroscopy unspecified; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-006803 SMASH-BIO | force_spectroscopy | study_record | in vitro Extending a spectrin repeat unit. II: rupture behavior. · Spectrin (repeats); afm smfs; experiment class: receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-007089 SMASH-BIO | force_spectroscopy | study_record | in vitro Unfolding a linker between helical repeats. · Spectrin (repeats); afm smfs; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-007147 SMASH-BIO | force_spectroscopy | study_record | in vitro Influence of lateral association on forced unfolding of antiparallel spectrin heterodimers. · Spectrin (repeats); afm smfs; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-007244 SMASH-BIO | force_spectroscopy | study_record | in vitro Cooperativity in forced unfolding of tandem spectrin repeats. · Spectrin (repeats); afm smfs; experiment class: rheology_cell, unfolding_protein | unmapped |
| EV-SMASH-SMFS-007254-r0 SMASH-BIO | force_spectroscopy | persistence_length: 2.5 nm | in vitro Elasticity of the human red blood cell skeleton. · Spectrin (repeats); optical tweezers; experiment class: rheology_cell | unmapped |
| EV-SMASH-SMFS-007291 SMASH-BIO | force_spectroscopy | study_record | in vitro Pathway shifts and thermal softening in temperature-coupled forced unfolding of spectrin domains. · Spectrin (repeats); force spectroscopy unspecified; experiment class: rheology_cell, unfolding_protein | unmapped |
| EV-SMASH-SMFS-007426 SMASH-BIO | force_spectroscopy | study_record | in vitro Direct measurement of the area expansion and shear moduli of the human red blood cell membrane skeleton. · Spectrin (repeats); optical tweezers; experiment class: rheology_cell | unmapped |
| EV-SMASH-SMFS-007500 SMASH-BIO | force_spectroscopy | study_record | in vitro Native topology determines force-induced unfolding pathways in globular proteins. · Spectrin (repeats); force spectroscopy unspecified; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-007521 SMASH-BIO | force_spectroscopy | study_record | in vitro States and transitions during forced unfolding of a single spectrin repeat. · Spectrin (repeats); afm smfs; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-007534 SMASH-BIO | force_spectroscopy | study_record | in vitro Unfolding proteins by external forces and temperature: the importance of topology and energetics. · Spectrin (repeats); optical tweezers; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-007546 SMASH-BIO | force_spectroscopy | study_record | in vitro Elasticity of the red cell membrane and its relation to hemolytic disorders: an optical tweezers study. · Spectrin (repeats); optical tweezers; experiment class: force_generation_motor, rheology_cell, unfolding_protein | unmapped |
| EV-SMASH-SMFS-007568 SMASH-BIO | force_spectroscopy | study_record | in vitro Single molecule force spectroscopy of spectrin repeats: low unfolding forces in helix bundles. · Spectrin (repeats); afm smfs; experiment class: unfolding_protein | unmapped |
| EV-SMASH-SMFS-007615 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 8 Regulation mechanism of the lateral diffusion of band 3 in erythrocyte membranes by the membrane skeleton. · Spectrin (repeats); optical tweezers; experiment class: rheology_cell | unmapped |
| EV-SMASH-SMFS-007668 SMASH-BIO | force_spectroscopy | study_record | in vitro In vitro motility of immunoadsorbed brain myosin-V using a Limulus acrosomal process and optical tweezer-based assay. · Spectrin (repeats); optical tweezers; experiment class: force_generation_motor, receptor_ligand_adhesion | unmapped |
| EV-SMASH-SMFS-007691-r0 SMASH-BIO | force_spectroscopy | persistence_length: 10 nm | in vitro Conformation and elasticity of the isolated red blood cell membrane skeleton. · Spectrin (repeats); optical tweezers; experiment class: membrane_mechanics, rheology_cell | unmapped |
Conflicts
No conflicts found among comparable evidence.
52 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.