Ribonuclease
UniProt P00648 · Bacillus amyloliquefaciens · 157 residues
71evidence items
69mapped to a state
2states
7techniques
5sources
0conflicts
Coverage grid
Mapped evidence per state and technique. Shaded rows rest on a single technique. Badge = best data level in the cell: raw (traces, images, FIDs: can be re-analysed), proc (maps, curves, tables, trajectories) or rep (values in the paper only).
| State | Basis | xray | nmr_structure | nmr_dynamics | nmr_shifts | force_spectroscopy | af_prediction | md | Total |
|---|---|---|---|---|---|---|---|---|---|
| native fold Barnase-native | observed | proc 45 | proc 6 | proc 1 | proc 9 | · | proc 1 | proc 2 | 64 |
| unfolded Barnase-unfolded | inferred (one method) | · | · | · | · | rep 5 | · | · | 5 |
Unmapped evidence: 2 of 71 items. Mappings come from curator assignments and curated rules; see About for how rules work.
Sources
| Source | Evidence | Mapped |
|---|---|---|
| AlphaFold DB | 1 | 1 |
| BMRB | 10 | 10 |
| MDDB | 2 | 2 |
| RCSB PDB | 53 | 51 |
| SMASH-BIO | 5 | 5 |
Curated rules applied
barnase-af-native: 1 itemsbarnase-force-unfolding: 5 itemsbarnase-md-native: 2 itemsbarnase-nmr-shifts-native: 10 itemsbarnase-structures-native: 50 items
Structure
Choose a state. Each model is badged observed or inferred; assumptions are one click away.
States and transitions
| State | Basis | Parent | Description |
|---|---|---|---|
| native fold Barnase-native | observed | — | Native fold of wild-type barnase, as in 1A2P (1.5 Å). |
| unfolded Barnase-unfolded | inferred | — | Unfolded ensemble reached by denaturant, temperature or force. No observed structure.Assumptions (2)
|
No transitions recorded in this version (no rate or free-energy evidence has been curated yet).
Evidence
One row per deposit or experiment. Filter by technique, environment or text. Unmapped evidence is listed too.
| Evidence | Technique | Observable | Conditions | Mapped to state |
|---|---|---|---|---|
| EV-PDB-1A2P-1 RCSB PDB | xray | resolution: 1.5 Å | in vitro · 13.85 °C · pH 7.5 · ligands ZN BARNASE WILDTYPE STRUCTURE AT 1.5 ANGSTROMS RESOLUTION · BARNASE (chains A,B,C) | native fold · curator:draft (unreviewed) · high Representative native structure; title and UniProt mapping checked against RCSB. |
| EV-PDB-1B20-1 RCSB PDB | xray | resolution: 1.7 Å | in vitro · -173.15 °C · pH 7.5 · ligands ZN DELETION OF A BURIED SALT-BRIDGE IN BARNASE · PROTEIN (BARNASE) (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1B21-1 RCSB PDB | xray | resolution: 2 Å | in vitro · -173.15 °C · pH 7.5 · ligands ZN DELETION OF A BURIED SALT BRIDGE IN BARNASE · PROTEIN (BARNASE) (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1B27-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · -173.15 °C · pH 8 STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE · PROTEIN (BARNASE) (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1B2S-1 RCSB PDB | xray | resolution: 1.82 Å | in vitro · -173.15 °C · pH 8 · ligands SO4 STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE · PROTEIN (BARNASE) (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1B2U-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · -173.15 °C · pH 6.5 STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE · PROTEIN (BARNASE) (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1B2X-1 RCSB PDB | xray | resolution: 1.8 Å | in vitro · -173.15 °C · pH 7.5 · ligands ZN BARNASE WILDTYPE STRUCTURE AT PH 7.5 FROM A CRYO_COOLED CRYSTAL AT 100K · PROTEIN (BARNASE) (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1B2Z-1 RCSB PDB | xray | resolution: 2.03 Å | in vitro · -173.15 °C · pH 7.5 · ligands ZN DELETION OF A BURIED SALT BRIDGE IN BARNASE · PROTEIN (BARNASE) (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1B3S-1 RCSB PDB | xray | resolution: 2.39 Å | in vitro · -173.15 °C · pH 8 STRUCTURAL RESPONSE TO MUTATION AT A PROTEIN-PROTEIN INTERFACE · PROTEIN (BARNASE) (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BAN-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro THE CONTRIBUTION OF BURIED HYDROGEN BONDS TO PROTEIN STABILITY: THE CRYSTAL STRUCTURES OF TWO BARNASE MUTANTS · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BAO-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · ligands ZN THE CONTRIBUTION OF BURIED HYDROGEN BONDS TO PROTEIN STABILITY: THE CRYSTAL STRUCTURES OF TWO BARNASE MUTANTS · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BGS-1 RCSB PDB | xray | resolution: 2.6 Å | in vitro RECOGNITION BETWEEN A BACTERIAL RIBONUCLEASE, BARNASE, AND ITS NATURAL INHIBITOR, BARSTAR · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BNE-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro BARNASE A43C/S80C DISULFIDE MUTANT · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BNF-1 RCSB PDB | xray | resolution: 2 Å | in vitro BARNASE T70C/S92C DISULFIDE MUTANT · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BNG-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro BARNASE S85C/H102C DISULFIDE MUTANT · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BNI-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · pH 6 BARNASE WILDTYPE STRUCTURE AT PH 6.0 · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BNJ-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · pH 9 BARNASE WILDTYPE STRUCTURE AT PH 9.0 · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BNR-1 RCSB PDB | nmr_structure | structure | in vitro BARNASE · BARNASE (G SPECIFIC ENDONUCLEASE) (chains A) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BNS-1 RCSB PDB | xray | resolution: 2.05 Å | in vitro STRUCTURAL STUDIES OF BARNASE MUTANTS · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BRG-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · ligands ZN CRYSTALLOGRAPHIC ANALYSIS OF PHE->LEU SUBSTITUTION IN THE HYDROPHOBIC CORE OF BARNASE · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BRH-1 RCSB PDB | xray | resolution: 2 Å | in vitro · ligands ZN BARNASE MUTANT WITH LEU 14 REPLACED BY ALA · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BRI-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro BARNASE MUTANT WITH ILE 76 REPLACED BY ALA · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BRJ-1 RCSB PDB | xray | resolution: 2 Å | in vitro · ligands ZN BARNASE MUTANT WITH ILE 88 REPLACED BY ALA · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BRK-1 RCSB PDB | xray | resolution: 2 Å | in vitro · ligands ZN BARNASE MUTANT WITH ILE 96 REPLACED BY ALA · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BRN-2 RCSB PDB | xray | resolution: 1.76 Å | in vitro SUBSITE BINDING IN AN RNASE: STRUCTURE OF A BARNASE-TETRANUCLEOTIDE COMPLEX AT 1.76 ANGSTROMS RESOLUTION · PROTEIN (BARNASE (E.C.3.1.27.-)) (chains L,M) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BRS-1 RCSB PDB | xray | resolution: 2 Å | in vitro PROTEIN-PROTEIN RECOGNITION: CRYSTAL STRUCTURAL ANALYSIS OF A BARNASE-BARSTAR COMPLEX AT 2.0-A RESOLUTION · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BSA-1 RCSB PDB | xray | resolution: 2 Å | in vitro CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BSC-1 RCSB PDB | xray | resolution: 2 Å | in vitro CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BSD-1 RCSB PDB | xray | resolution: 2.3 Å | in vitro CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BSE-1 RCSB PDB | xray | resolution: 2 Å | in vitro CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1FW7-1 RCSB PDB | nmr_structure | structure | in vitro · 29.85 °C · pH 6.5 NMR STRUCTURE OF 15N-LABELED BARNASE · BARNASE (chains A) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1RNB-2 RCSB PDB | xray | resolution: 1.9 Å | in vitro · ligands SO4 CRYSTAL STRUCTURE OF A BARNASE-D(*GP*C) COMPLEX AT 1.9 ANGSTROMS RESOLUTION · BARNASE (chains A) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1X1U-1 RCSB PDB | xray | resolution: 2.3 Å | in vitro · -173.15 °C Water-mediate interaction at aprotein-protein interface · Ribonuclease (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1X1W-1 RCSB PDB | xray | resolution: 2.1 Å | in vitro · -173.15 °C Water-mediate interaction at aprotein-protein interface · Ribonuclease (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1X1X-1 RCSB PDB | xray | resolution: 2.3 Å | in vitro · -173.15 °C Water-mediate interaction at aprotein-protein interface · Ribonuclease (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1X1Y-1 RCSB PDB | xray | resolution: 1.9 Å | in vitro · -173.15 °C Water-mediate interaction at aprotein-protein interface · Ribonuclease (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1YVS-1 RCSB PDB | xray | resolution: 2.2 Å | in vitro · 13.85 °C · pH 4.5 · ligands SO4 Trimeric domain swapped barnase · BARNASE (chains A) | unmapped |
| EV-PDB-2C4B-1 RCSB PDB | xray | resolution: 1.3 Å | in vitro · -173.15 °C · ligands 2PE, EDO, FMT, GOL, MES, SO4, UNX Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A · BARNASE MCOEETI FUSION (chains A,B) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-2F4Y-1 RCSB PDB | xray | resolution: 2.15 Å | in vitro · 3.85 °C · pH 8 · ligands ZN Barnase cross-linked with glutaraldehyde · Ribonuclease (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-2F56-1 RCSB PDB | xray | resolution: 1.955 Å | in vitro · 3.85 °C · pH 8 · ligands URE, ZN Barnase cross-linked with glutaraldehyde soaked in 6M urea · Ribonuclease (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-2F5M-1 RCSB PDB | xray | resolution: 1.95 Å | in vitro · 3.85 °C · pH 8 · ligands BRJ Cross-linked barnase soaked in bromo-ethanol · Ribonuclease (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-2F5W-1 RCSB PDB | xray | resolution: 2.001 Å | in vitro · 3.85 °C · pH 8 · ligands TOU, ZN Cross-linked barnase soaked in 3 M thiourea · Ribonuclease (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-2KF3-1 RCSB PDB | nmr_structure | structure | in vitro · 24.85 °C · pH 6.7 Barnase, low pressure reference NMR structure · Ribonuclease (chains A) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-2KF5-1 RCSB PDB | nmr_structure | structure | in vitro · 24.85 °C · pH 6.7 Barnase bound to d(CGAC), low pressure · Ribonuclease (chains A) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-2KF6-1 RCSB PDB | nmr_structure | structure | in vitro · 24.85 °C · pH 6.7 Barnase bound to d(CGAC) high pressure · Ribonuclease (chains A) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-2ZA4-1 RCSB PDB | xray | resolution: 1.58 Å | in vitro · -178.15 °C · pH 7 · ligands CL Crystal Structural Analysis of Barnase-barstar Complex · Ribonuclease (chains A,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-3DA7-1 RCSB PDB | xray | resolution: 2.25 Å | in vitro · -173.15 °C · pH 8.5 A conformationally strained, circular permutant of barnase · Barnase circular permutant (chains A,B,E,G) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-3KCH-1 RCSB PDB | xray | resolution: 1.94 Å | in vitro · 3.85 °C · pH 7.5 · ligands PTD Baranase crosslinked by glutaraldehyde · Ribonuclease (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-2KF4-1 RCSB PDB | nmr_structure | structure | in vitro · 24.85 °C · pH 6.7 Barnase high pressure structure · Ribonuclease (chains A) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-1BSB-1 RCSB PDB | xray | resolution: 2 Å | in vitro CRYSTAL STRUCTURAL ANALYSIS OF MUTATIONS IN THE HYDROPHOBIC CORES OF BARNASE · BARNASE (chains A,B,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-3Q3F-1 RCSB PDB | xray | resolution: 2.169 Å | in vitro · -173.15 °C · pH 8 · ligands SO4 Engineering Domain-Swapped Binding Interfaces by Mutually Exclusive Folding: Insertion of Ubiquitin into position 103 of Barnase · Ribonuclease/Ubiquitin chimeric protein (chains A) | unmapped |
| EV-PDB-6PQK-1 RCSB PDB | xray | resolution: 1.2 Å | in vitro · -193.15 °C · pH 6.5 · ligands EDO, PO4 Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/S59C/A67C/C82A · Ribonuclease (chains A,C) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-PDB-7MRX-1 RCSB PDB | xray | resolution: 2.29 Å | in vitro · -193.15 °C Cryogenic crystal structure of barnase A43C/S80C bound to barstar C40A/C82A · Ribonuclease (chains A,C,E) | native fold · rule:barnase-structures-native · medium Experimental structure of this small single-domain protein; deposited structures show the native fold unless the title says otherwise. |
| EV-BMRB-16169 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 6.7 Barnase low pressure reference · BMRB data: 1H chemical shifts | native fold · rule:barnase-nmr-shifts-native · low Chemical shift assignment of the folded protein; shift-only entries rarely describe non-native states unless titled so. |
| EV-BMRB-16170 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 6.7 Barnase high pressure structure · BMRB data: 1H chemical shifts | native fold · rule:barnase-nmr-shifts-native · low Chemical shift assignment of the folded protein; shift-only entries rarely describe non-native states unless titled so. |
| EV-BMRB-16171 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 6.7 Barnase bound to d(CGAC), low pressure · BMRB data: 1H chemical shifts | native fold · rule:barnase-nmr-shifts-native · low Chemical shift assignment of the folded protein; shift-only entries rarely describe non-native states unless titled so. |
| EV-BMRB-16172 BMRB | nmr_shifts | chemical_shift | in vitro · 24.85 °C · pH 6.7 Barnase bound to d(CGAC) high pressure · BMRB data: 1H chemical shifts | native fold · rule:barnase-nmr-shifts-native · low Chemical shift assignment of the folded protein; shift-only entries rarely describe non-native states unless titled so. |
| EV-BMRB-2066 BMRB | nmr_shifts | chemical_shift | in vitro · 36.85 °C · pH 4.5 Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR · BMRB data: 1H chemical shifts | native fold · rule:barnase-nmr-shifts-native · low Chemical shift assignment of the folded protein; shift-only entries rarely describe non-native states unless titled so. |
| EV-BMRB-2067 BMRB | nmr_shifts | chemical_shift | in vitro · 36.85 °C · pH 4.5 Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR · BMRB data: 1H chemical shifts | native fold · rule:barnase-nmr-shifts-native · low Chemical shift assignment of the folded protein; shift-only entries rarely describe non-native states unless titled so. |
| EV-BMRB-2070 BMRB | nmr_shifts | chemical_shift | in vitro · 26.85 °C · pH 4.5 Characterization of Phosphate Binding in the Active Site of Barnase by Site-Directed Mutagenesis and NMR · BMRB data: 1H chemical shifts | native fold · rule:barnase-nmr-shifts-native · low Chemical shift assignment of the folded protein; shift-only entries rarely describe non-native states unless titled so. |
| EV-BMRB-4964 BMRB | nmr_shifts | chemical_shift | in vitro · 29.85 °C · pH 6.7 1H, 13C and 15N resonance assignment for barnase · BMRB data: 1H chemical shifts, 13C chemical shifts, 15N chemical shifts | native fold · rule:barnase-nmr-shifts-native · low Chemical shift assignment of the folded protein; shift-only entries rarely describe non-native states unless titled so. |
| EV-BMRB-7139 BMRB | nmr_dynamics | relaxation_rate | in vitro · 29.85 °C · pH 6.7 1H,13C and 15N resonance assignments of barnase-barstar complex NMR deuterium methyl relaxation data for free barnase and barnase-barstar complex · BMRB data: order parameters | native fold · rule:barnase-nmr-shifts-native · low Chemical shift assignment of the folded protein; shift-only entries rarely describe non-native states unless titled so. |
| EV-BMRB-975 BMRB | nmr_shifts | chemical_shift | in vitro · 36.85 °C · pH 4.5 Sequential Assignment of the 1H Nuclear Magnetic Resonance Spectrum of Barnase · BMRB data: 1H chemical shifts | native fold · rule:barnase-nmr-shifts-native · low Chemical shift assignment of the folded protein; shift-only entries rarely describe non-native states unless titled so. |
| EV-AFDB-AF-P00648-F1 AlphaFold DB | af_prediction | plddt: 84.5 pLDDT | in silico AlphaFold DB prediction AF-P00648-F1 · AlphaFold Monomer v2.0 pipeline, residues 1-157 | native fold · rule:barnase-af-native · low AlphaFold prediction of the native fold (prediction, not a measurement). |
| EV-MDDB-A003U MDDB | md | trajectory: 10 ns | in silico · 26.85 °C MoDEL 1A2P · start 1A2P; Amber Parm99 | native fold · rule:barnase-md-native · low Simulation started from a native structure (prediction). |
| EV-MDDB-A006F MDDB | md | trajectory: 10 ns | in silico · 26.85 °C MoDEL 1BGS · start 1BGS; Amber Parm99 | native fold · rule:barnase-md-native · low Simulation started from a native structure (prediction). |
| EV-SMASH-SMFS-001622 SMASH-BIO | force_spectroscopy | study_record | in vitro · pH 7 Molten globule-like transition state of protein barnase measured with calorimetric force spectroscopy. · Barnase; optical tweezers; experiment class: unfolding_protein | unfolded · rule:barnase-force-unfolding · low SMASH-BIO force record classed as protein unfolding; mapped to the unfolded state the molecule reaches. Most SMASH-BIO values are extracted automatically. |
| EV-SMASH-SMFS-001925 SMASH-BIO | force_spectroscopy | study_record | in vitro Force Dependence of Proteins' Transition State Position and the Bell-Evans Model. · Barnase; afm smfs; experiment class: nucleic_acid_mechanics, unfolding_nucleic_acid, unfolding_protein | unfolded · rule:barnase-force-unfolding · low SMASH-BIO force record classed as protein unfolding; mapped to the unfolded state the molecule reaches. Most SMASH-BIO values are extracted automatically. |
| EV-SMASH-SMFS-003949 SMASH-BIO | force_spectroscopy | study_record | in vitro Mechanical Folding and Unfolding of Protein Barnase at the Single-Molecule Level. · Barnase; optical tweezers; experiment class: unfolding_protein | unfolded · rule:barnase-force-unfolding · low SMASH-BIO force record classed as protein unfolding; mapped to the unfolded state the molecule reaches. Most SMASH-BIO values are extracted automatically. |
| EV-SMASH-SMFS-005798 SMASH-BIO | force_spectroscopy | study_record | in vitro Force spectroscopy of barnase-barstar single molecule interaction. · Barnase; afm smfs; experiment class: unfolding_protein | unfolded · rule:barnase-force-unfolding · low SMASH-BIO force record classed as protein unfolding; mapped to the unfolded state the molecule reaches. Most SMASH-BIO values are extracted automatically. |
| EV-SMASH-SMFS-007419 SMASH-BIO | force_spectroscopy | study_record | in vitro Can non-mechanical proteins withstand force? Stretching barnase by atomic force microscopy and molecular dynamics simulation. · Barnase; afm smfs; experiment class: unfolding_protein | unfolded · rule:barnase-force-unfolding · low SMASH-BIO force record classed as protein unfolding; mapped to the unfolded state the molecule reaches. Most SMASH-BIO values are extracted automatically. |
Conflicts
No conflicts found among comparable evidence.
71 evidence items could not be compared: they lack a comparable observable type, a value, or an uncertainty. Absence of a conflict here is not agreement.
Downloads
- Protein bundle (JSON)
- Barnase-native-cif (mmCIF, observed)
- Barnase-native-pdb (PDB, observed)
- Curation file (YAML)