{ "conflicts": [], "coverage": { "cells": {}, "data_levels": {}, "single_method_states": [], "states": [], "techniques": [ "xray", "cryoem", "nmr_shifts", "force_spectroscopy", "af_prediction" ], "unmapped_evidence": 161 }, "curation": { "as_of": "", "curator": "none", "file": "", "status": "uncurated" }, "evidence": [ { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4UN4", "evidence_id": "EV-PDB-4UN4-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.371 }, "provenance": { "deposit_id": "4UN4", "doi": "10.1038/NATURE13579", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4UN4" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Cas9 bound to PAM-containing DNA target with mismatches at positions 1-2" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4UN5", "evidence_id": "EV-PDB-4UN5-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.4 }, "provenance": { "deposit_id": "4UN5", "doi": "10.1038/NATURE13579", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4UN5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Cas9 bound to PAM-containing DNA target containing mismatches at positions 1-3" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9 (chains A,C)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4ZT0", "evidence_id": "EV-PDB-4ZT0-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.9 }, "provenance": { "deposit_id": "4ZT0", "doi": "10.1126/science.aab1452", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4ZT0" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of catalytically-active Streptococcus pyogenes Cas9 in complex with single-guide RNA at 2.9 Angstrom resolution" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9 (chains A,C)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4ZT9", "evidence_id": "EV-PDB-4ZT9-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.1 }, "provenance": { "deposit_id": "4ZT9", "doi": "10.1126/science.aab1452", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4ZT9" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Nuclease-inactive Streptococcus pyogenes Cas9 (D10A/H840A, dCas9) in complex with single-guide RNA at 3.1 Angstrom resolution" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6IFO", "evidence_id": "EV-PDB-6IFO-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.313 }, "provenance": { "deposit_id": "6IFO", "doi": "10.1016/j.molcel.2018.11.011", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6IFO" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of AcrIIA2-SpyCas9-sgRNA ternary complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -188.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6K4S", "evidence_id": "EV-PDB-6K4S-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.01 }, "provenance": { "deposit_id": "6K4S", "doi": "10.1371/journal.pbio.3000496", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6K4S" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of xCas9 in complex with sgRNA and DNA (TGC PAM)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QQX", "evidence_id": "EV-PDB-7QQX-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.4 }, "provenance": { "deposit_id": "7QQX", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QQX" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to FANCF off-target5 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QQW", "evidence_id": "EV-PDB-7QQW-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.1 }, "provenance": { "deposit_id": "7QQW", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QQW" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to FANCF off-target4 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QQV", "evidence_id": "EV-PDB-7QQV-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.25 }, "provenance": { "deposit_id": "7QQV", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QQV" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to FANCF off-target3 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QQU", "evidence_id": "EV-PDB-7QQU-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.45 }, "provenance": { "deposit_id": "7QQU", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QQU" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to FANCF off-target2 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QQT", "evidence_id": "EV-PDB-7QQT-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.5 }, "provenance": { "deposit_id": "7QQT", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QQT" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to FANCF off-target1 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QQS", "evidence_id": "EV-PDB-7QQS-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.4 }, "provenance": { "deposit_id": "7QQS", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QQS" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to FANCF on-target DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "EDO", "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QQR", "evidence_id": "EV-PDB-7QQR-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.75 }, "provenance": { "deposit_id": "7QQR", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QQR" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to AAVS1 off-target5 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "EDO", "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QQQ", "evidence_id": "EV-PDB-7QQQ-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.65 }, "provenance": { "deposit_id": "7QQQ", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QQQ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to AAVS1 off-target4 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "EDO", "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QQP", "evidence_id": "EV-PDB-7QQP-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.6 }, "provenance": { "deposit_id": "7QQP", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QQP" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to AAVS1 off-target3 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QQO", "evidence_id": "EV-PDB-7QQO-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.0 }, "provenance": { "deposit_id": "7QQO", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QQO" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to AAVS1 off-target1 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7OXA", "evidence_id": "EV-PDB-7OXA-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.15 }, "provenance": { "deposit_id": "7OXA", "doi": "10.1016/j.molcel.2021.07.035", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7OXA" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Target-bound SpCas9 complex with AAVS1 chimeric RNA-DNA guide" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7OX9", "evidence_id": "EV-PDB-7OX9-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.45 }, "provenance": { "deposit_id": "7OX9", "doi": "10.1016/j.molcel.2021.07.035", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7OX9" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Target-bound SpCas9 complex with AAVS1 all-RNA guide" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7OX8", "evidence_id": "EV-PDB-7OX8-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.75 }, "provenance": { "deposit_id": "7OX8", "doi": "10.1016/j.molcel.2021.07.035", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7OX8" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Target-bound SpCas9 complex with TRAC full RNA guide" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7OX7", "evidence_id": "EV-PDB-7OX7-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.6 }, "provenance": { "deposit_id": "7OX7", "doi": "10.1016/j.molcel.2021.07.035", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7OX7" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Target-bound SpCas9 complex with TRAC chimeric RNA-DNA guide" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG", "ZN" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9 (chains B)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6VPC", "evidence_id": "EV-PDB-6VPC-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.2 }, "provenance": { "deposit_id": "6VPC", "doi": "10.1126/science.abb1390", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6VPC" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Structure of the SpCas9 DNA adenine base editor - ABE8e" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6O56", "evidence_id": "EV-PDB-6O56-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "6O56", "doi": "10.1021/jacs.9b10521", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6O56" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "HNH Nuclease from S. pyogenes Cas9" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6O0Z", "evidence_id": "EV-PDB-6O0Z-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.3 }, "provenance": { "deposit_id": "6O0Z", "doi": "10.1038/s41594-019-0258-2", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6O0Z" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6O0Y", "evidence_id": "EV-PDB-6O0Y-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.37 }, "provenance": { "deposit_id": "6O0Y", "doi": "10.1038/s41594-019-0258-2", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6O0Y" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6O0X", "evidence_id": "EV-PDB-6O0X-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.28 }, "provenance": { "deposit_id": "6O0X", "doi": "10.1038/s41594-019-0258-2", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6O0X" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Conformational states of Cas9-sgRNA-DNA ternary complex in the presence of magnesium" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6MCC", "evidence_id": "EV-PDB-6MCC-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.9 }, "provenance": { "deposit_id": "6MCC", "doi": "10.1016/j.molcel.2018.11.016", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6MCC" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "CryoEM structure of AcrIIA2 homolog in complex with CRISPR-Cas9" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -188.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6K57", "evidence_id": "EV-PDB-6K57-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.98 }, "provenance": { "deposit_id": "6K57", "doi": "10.1371/journal.pbio.3000496", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6K57" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of dCas9 in complex with sgRNA and DNA (CGA PAM)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -188.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6K4U", "evidence_id": "EV-PDB-6K4U-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.2 }, "provenance": { "deposit_id": "6K4U", "doi": "10.1371/journal.pbio.3000496", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6K4U" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of xCas9 in complex with sgRNA and DNA (TGA PAM)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "PO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -188.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6K4Q", "evidence_id": "EV-PDB-6K4Q-4", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.7 }, "provenance": { "deposit_id": "6K4Q", "doi": "10.1371/journal.pbio.3000496", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6K4Q" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of xCas9 in complex with sgRNA and DNA (CGG PAM)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "PO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -188.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6K4P", "evidence_id": "EV-PDB-6K4P-4", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.9 }, "provenance": { "deposit_id": "6K4P", "doi": "10.1371/journal.pbio.3000496", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6K4P" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of xCas9 in complex with sgRNA and DNA (TGG PAM)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -188.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6K3Z", "evidence_id": "EV-PDB-6K3Z-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.2 }, "provenance": { "deposit_id": "6K3Z", "doi": "10.1371/journal.pbio.3000496", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6K3Z" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of dCas9 in complex with sgRNA and DNA (TGA PAM)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "EDO", "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6AI6", "evidence_id": "EV-PDB-6AI6-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.7 }, "provenance": { "deposit_id": "6AI6", "doi": "10.1126/science.aas9129", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6AI6" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of SpCas9-NG" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "DNA nuclease (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6AEG", "evidence_id": "EV-PDB-6AEG-4", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.701 }, "provenance": { "deposit_id": "6AEG", "doi": "10.1038/s41422-018-0131-6", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6AEG" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of xCas9 in complex with sgRNA and target DNA (GAT PAM)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "DNA Nuclease (chains B,F)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6AEB", "evidence_id": "EV-PDB-6AEB-4", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.004 }, "provenance": { "deposit_id": "6AEB", "doi": "10.1038/s41422-018-0131-6", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6AEB" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of xCas9 in complex with sgRNA and target DNA (AAG PAM)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5Y36", "evidence_id": "EV-PDB-5Y36-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 5.2 }, "provenance": { "deposit_id": "5Y36", "doi": "10.1038/s41467-017-01496-2", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5Y36" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Cryo-EM structure of SpCas9-sgRNA-DNA ternary complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5XBL", "evidence_id": "EV-PDB-5XBL-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.052 }, "provenance": { "deposit_id": "5XBL", "doi": "10.1038/nature22377", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5XBL" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of nuclease in complex with associated protein" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5VZL", "evidence_id": "EV-PDB-5VZL-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.9 }, "provenance": { "deposit_id": "5VZL", "doi": "10.1126/sciadv.1701620", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5VZL" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "cryo-EM structure of the Cas9-sgRNA-AcrIIA4 anti-CRISPR complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "EDO", "MG", "PEG", "PG4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.2, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5VW1", "evidence_id": "EV-PDB-5VW1-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.598 }, "provenance": { "deposit_id": "5VW1", "doi": "10.1016/j.molcel.2017.05.024", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5VW1" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of SpyCas9-sgRNA-AcrIIA4 ternary complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5FW3", "evidence_id": "EV-PDB-5FW3-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.7 }, "provenance": { "deposit_id": "5FW3", "doi": "10.1016/J.MOLCEL.2016.02.020", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5FW3" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of SpCas9 variant VRER bound to sgRNA and TGCG PAM target DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5FW2", "evidence_id": "EV-PDB-5FW2-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.676 }, "provenance": { "deposit_id": "5FW2", "doi": "10.1016/J.MOLCEL.2016.02.020", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5FW2" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of SpCas9 variant EQR bound to sgRNA and TGAG PAM target DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5FW1", "evidence_id": "EV-PDB-5FW1-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.499 }, "provenance": { "deposit_id": "5FW1", "doi": "10.1016/J.MOLCEL.2016.02.020", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5FW1" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of SpyCas9 variant VQR bound to sgRNA and TGAG PAM target DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5FQ5", "evidence_id": "EV-PDB-5FQ5-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.136 }, "provenance": { "deposit_id": "5FQ5", "doi": "10.1107/S2059798315024110", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5FQ5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Cas9-sgRNA-DNA complex solved by native SAD phasing" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5F9R", "evidence_id": "EV-PDB-5F9R-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.4 }, "provenance": { "deposit_id": "5F9R", "doi": "10.1126/science.aad8282", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5F9R" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of catalytically-active Streptococcus pyogenes CRISPR-Cas9 in complex with single-guided RNA and double-stranded DNA primed for target DNA cleavage" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "ACT", "EDO", "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5B2T", "evidence_id": "EV-PDB-5B2T-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.2 }, "provenance": { "deposit_id": "5B2T", "doi": "10.1016/j.molcel.2016.02.018", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5B2T" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of the Streptococcus pyogenes Cas9 VRER variant in complex with sgRNA and target DNA (TGCG PAM)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "ACT", "EDO", "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5B2S", "evidence_id": "EV-PDB-5B2S-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.2 }, "provenance": { "deposit_id": "5B2S", "doi": "10.1016/j.molcel.2016.02.018", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5B2S" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of the Streptococcus pyogenes Cas9 EQR variant in complex with sgRNA and target DNA (TGAG PAM)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "ACT", "EDO", "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5B2R", "evidence_id": "EV-PDB-5B2R-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "5B2R", "doi": "10.1016/j.molcel.2016.02.018", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5B2R" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of the Streptococcus pyogenes Cas9 VQR variant in complex with sgRNA and target DNA (TGA PAM)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4UN3", "evidence_id": "EV-PDB-4UN3-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.593 }, "provenance": { "deposit_id": "4UN3", "doi": "10.1038/NATURE13579", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4UN3" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Cas9 bound to PAM-containing DNA target" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A,D)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4OO8", "evidence_id": "EV-PDB-4OO8-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.5 }, "provenance": { "deposit_id": "4OO8", "doi": "10.1016/j.cell.2014.02.001", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4OO8" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Streptococcus pyogenes Cas9 in complex with guide RNA and target DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4CMQ", "evidence_id": "EV-PDB-4CMQ-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.09 }, "provenance": { "deposit_id": "4CMQ", "doi": "10.1126/SCIENCE.1247997", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4CMQ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Mn-bound S.pyogenes Cas9" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-ASSOCIATED ENDONUCLEASE CAS9/CSN1 (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4CMP", "evidence_id": "EV-PDB-4CMP-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.62 }, "provenance": { "deposit_id": "4CMP", "doi": "10.1126/SCIENCE.1247997", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4CMP" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of S. pyogenes Cas9" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QQZ", "evidence_id": "EV-PDB-7QQZ-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.25 }, "provenance": { "deposit_id": "7QQZ", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QQZ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to FANCF off-target7 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QR1", "evidence_id": "EV-PDB-7QR1-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.6 }, "provenance": { "deposit_id": "7QR1", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QR1" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to TRAC off-target2 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "EDO", "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QR5", "evidence_id": "EV-PDB-7QR5-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.3 }, "provenance": { "deposit_id": "7QR5", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QR5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to FANCF off-target6 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QR7", "evidence_id": "EV-PDB-7QR7-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.0 }, "provenance": { "deposit_id": "7QR7", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QR7" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to AAVS1 off-target2 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains P)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7S3H", "evidence_id": "EV-PDB-7S3H-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.5 }, "provenance": { "deposit_id": "7S3H", "doi": "10.1038/s41594-022-00756-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7S3H" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Cas9:sgRNA:DNA (S. pyogenes) with 0 RNA:DNA base pairs, open-protein/linear-DNA conformation" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B,E)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7V59", "evidence_id": "EV-PDB-7V59-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 5.26 }, "provenance": { "deposit_id": "7V59", "doi": "10.1039/d1sc02633j", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7V59" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Cryo-EM structure of spyCas9-sgRNA-DNA dimer" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7ZO1", "evidence_id": "EV-PDB-7ZO1-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.4 }, "provenance": { "deposit_id": "7ZO1", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7ZO1" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to CD34 off-target9 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8KAG", "evidence_id": "EV-PDB-8KAG-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.88 }, "provenance": { "deposit_id": "8KAG", "doi": "10.1038/s41587-024-02255-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8KAG" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of SpyCas9 in complex with sgRNA and target RNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8KAM", "evidence_id": "EV-PDB-8KAM-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.91 }, "provenance": { "deposit_id": "8KAM", "doi": "10.1038/s41587-024-02255-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8KAM" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of SpyCas9 in complex with sgRNA and 16nt target DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8SRS", "evidence_id": "EV-PDB-8SRS-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.79 }, "provenance": { "deposit_id": "8SRS", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8SRS" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRY-Cas9:gRNA complex targeting TAC PAM DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8T6X", "evidence_id": "EV-PDB-8T6X-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.08 }, "provenance": { "deposit_id": "8T6X", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8T6X" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 18 bp R-loop" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8TZZ", "evidence_id": "EV-PDB-8TZZ-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.56 }, "provenance": { "deposit_id": "8TZZ", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8TZZ" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpG Cas9 with NGC PAM DNA target" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A,G)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8T7S", "evidence_id": "EV-PDB-8T7S-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.01 }, "provenance": { "deposit_id": "8T7S", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8T7S" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRYmer bound to NAC PAM DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8T79", "evidence_id": "EV-PDB-8T79-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.04 }, "provenance": { "deposit_id": "8T79", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8T79" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRY-Cas9:gRNA complex bound to non-target DNA with 10 bp R-loop" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8T78", "evidence_id": "EV-PDB-8T78-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.02 }, "provenance": { "deposit_id": "8T78", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8T78" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRY-Cas9:gRNA complex bound to non-target DNA with 8 bp R-loop" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8T77", "evidence_id": "EV-PDB-8T77-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.25 }, "provenance": { "deposit_id": "8T77", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8T77" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRY-Cas9:gRNA complex bound to non-target DNA with 6 bp R-loop" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8T76", "evidence_id": "EV-PDB-8T76-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.42 }, "provenance": { "deposit_id": "8T76", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8T76" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 3 bp R-loop" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8T6Y", "evidence_id": "EV-PDB-8T6Y-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.88 }, "provenance": { "deposit_id": "8T6Y", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8T6Y" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRY-Cas9:gRNA complex bound to non-target DNA with 1 bp R-loop" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8T6T", "evidence_id": "EV-PDB-8T6T-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.04 }, "provenance": { "deposit_id": "8T6T", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8T6T" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 13 bp R-loop" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8T6S", "evidence_id": "EV-PDB-8T6S-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.28 }, "provenance": { "deposit_id": "8T6S", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8T6S" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 10 bp R-loop" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8T6P", "evidence_id": "EV-PDB-8T6P-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.15 }, "provenance": { "deposit_id": "8T6P", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8T6P" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 2 bp R-loop" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8T6O", "evidence_id": "EV-PDB-8T6O-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.1 }, "provenance": { "deposit_id": "8T6O", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8T6O" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 0 bp R-loop" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8SQH", "evidence_id": "EV-PDB-8SQH-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.69 }, "provenance": { "deposit_id": "8SQH", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8SQH" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRY-Cas9:gRNA complex targeting TTC PAM DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8SPQ", "evidence_id": "EV-PDB-8SPQ-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.26 }, "provenance": { "deposit_id": "8SPQ", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8SPQ" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpRY-Cas9:gRNA complex targeting TGG PAM DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "EDO" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8SCA", "evidence_id": "EV-PDB-8SCA-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.67 }, "provenance": { "deposit_id": "8SCA", "doi": "10.1126/sciadv.adl1045", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8SCA" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Rec3 Domain from S. pyogenes Cas9" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B,G)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8KAL", "evidence_id": "EV-PDB-8KAL-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.16 }, "provenance": { "deposit_id": "8KAL", "doi": "10.1038/s41587-024-02255-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8KAL" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of SpyCas9 in complex with sgRNA and 17nt target DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B,F)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8KAK", "evidence_id": "EV-PDB-8KAK-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.6 }, "provenance": { "deposit_id": "8KAK", "doi": "10.1038/s41587-024-02255-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8KAK" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of SpyCas9 in complex with sgRNA and 18nt target DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B,F)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8KAJ", "evidence_id": "EV-PDB-8KAJ-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.42 }, "provenance": { "deposit_id": "8KAJ", "doi": "10.1038/s41587-024-02255-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8KAJ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of SpyCas9-crRNA-tracrRNA complex bound to 16nt target DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B,F)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8KAI", "evidence_id": "EV-PDB-8KAI-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.49 }, "provenance": { "deposit_id": "8KAI", "doi": "10.1038/s41587-024-02255-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8KAI" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of SpyCas9-crRNA-tracrRNA complex bound to 17nt target DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B,F)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8KAH", "evidence_id": "EV-PDB-8KAH-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.36 }, "provenance": { "deposit_id": "8KAH", "doi": "10.1038/s41587-024-02255-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8KAH" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of SpyCas9-crRNA-tracrRNA complex bound to 18nt target DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8G1I", "evidence_id": "EV-PDB-8G1I-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.12 }, "provenance": { "deposit_id": "8G1I", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8G1I" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 with sgRNA and target DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains E)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8FZT", "evidence_id": "EV-PDB-8FZT-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.03 }, "provenance": { "deposit_id": "8FZT", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8FZT" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 with dual-guide RNA and target DNA" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7Z4L", "evidence_id": "EV-PDB-7Z4L-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.54 }, "provenance": { "deposit_id": "7Z4L", "doi": "10.1038/s41586-022-05114-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7Z4L" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 bound to 18-nucleotide complementary DNA substrate in the checkpoint state" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7Z4K", "evidence_id": "EV-PDB-7Z4K-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.81 }, "provenance": { "deposit_id": "7Z4K", "doi": "10.1038/s41586-022-05114-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7Z4K" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 bound to 10-nucleotide complementary DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7Z4J", "evidence_id": "EV-PDB-7Z4J-5", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.99 }, "provenance": { "deposit_id": "7Z4J", "doi": "10.1038/s41586-022-05114-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7Z4J" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 bound to 18-nucleotide complementary DNA substrate in the catalytic state" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7Z4I", "evidence_id": "EV-PDB-7Z4I-4", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.12 }, "provenance": { "deposit_id": "7Z4I", "doi": "10.1038/s41586-022-05114-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7Z4I" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 bound to 16-nucleotide complementary DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7Z4H", "evidence_id": "EV-PDB-7Z4H-4", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.49 }, "provenance": { "deposit_id": "7Z4H", "doi": "10.1038/s41586-022-05114-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7Z4H" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 bound to 14-nucleotide complementary DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7Z4G", "evidence_id": "EV-PDB-7Z4G-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.64 }, "provenance": { "deposit_id": "7Z4G", "doi": "10.1038/s41586-022-05114-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7Z4G" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 bound to 12-nucleotide complementary DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7Z4E", "evidence_id": "EV-PDB-7Z4E-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 4.14 }, "provenance": { "deposit_id": "7Z4E", "doi": "10.1038/s41586-022-05114-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7Z4E" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 bound to 8-nucleotide complementary DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B,E)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7Z4D", "evidence_id": "EV-PDB-7Z4D-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.1 }, "provenance": { "deposit_id": "7Z4D", "doi": "10.1038/s41586-022-05114-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7Z4D" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of SpCas9 bound to a 10 nucleotide complementary DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7Z4C", "evidence_id": "EV-PDB-7Z4C-4", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.87 }, "provenance": { "deposit_id": "7Z4C", "doi": "10.1038/s41586-022-05114-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7Z4C" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 bound to 6 nucleotide complementary DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7VK9", "evidence_id": "EV-PDB-7VK9-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.9 }, "provenance": { "deposit_id": "7VK9", "doi": "10.1021/acscatal.2c01804", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7VK9" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of xCas9 P411T" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7S4X", "evidence_id": "EV-PDB-7S4X-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.76 }, "provenance": { "deposit_id": "7S4X", "doi": "10.1038/s41586-022-04470-1", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7S4X" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Cas9:gRNA in complex with 18-20MM DNA, 1 minute time-point, kinked active conformation" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7S4V", "evidence_id": "EV-PDB-7S4V-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.28 }, "provenance": { "deposit_id": "7S4V", "doi": "10.1038/s41586-022-04470-1", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7S4V" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Cas9 bound to 12-14MM DNA, 60 min time-point, kinked conformation" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7S4U", "evidence_id": "EV-PDB-7S4U-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.56 }, "provenance": { "deposit_id": "7S4U", "doi": "10.1038/s41586-022-04470-1", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7S4U" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Cryo-EM structure of Cas9 in complex with 12-14MM DNA substrate, 5 minute time-point" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains P)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7S38", "evidence_id": "EV-PDB-7S38-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.3 }, "provenance": { "deposit_id": "7S38", "doi": "10.1038/s41594-022-00756-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7S38" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Cas9:sgRNA:DNA (S. pyogenes) forming a 3-base-pair R-loop" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains P)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7S37", "evidence_id": "EV-PDB-7S37-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.2 }, "provenance": { "deposit_id": "7S37", "doi": "10.1038/s41594-022-00756-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7S37" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Cas9:sgRNA (S. pyogenes) in the open-protein conformation" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains P)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7S36", "evidence_id": "EV-PDB-7S36-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.2 }, "provenance": { "deposit_id": "7S36", "doi": "10.1038/s41594-022-00756-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7S36" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Cas9:sgRNA:DNA (S. pyogenes) with 0 RNA:DNA base pairs, closed-protein/bent-DNA conformation" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QR8", "evidence_id": "EV-PDB-7QR8-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.75 }, "provenance": { "deposit_id": "7QR8", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QR8" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to PTPRC off-target1 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "K", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/7QR0", "evidence_id": "EV-PDB-7QR0-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.3 }, "provenance": { "deposit_id": "7QR0", "doi": "10.1016/j.cell.2022.09.026", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/7QR0" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SpCas9 bound to TRAC off-target1 DNA substrate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8U3Y", "evidence_id": "EV-PDB-8U3Y-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.1 }, "provenance": { "deposit_id": "8U3Y", "doi": "10.1038/s41467-024-47830-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8U3Y" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpG Cas9 with NGG PAM DNA target" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8WUS", "evidence_id": "EV-PDB-8WUS-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.9 }, "provenance": { "deposit_id": "8WUS", "doi": "10.1038/s41586-024-07497-8", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8WUS" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9-MMLV RT-pegRNA-target DNA complex (termination)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8WUV", "evidence_id": "EV-PDB-8WUV-3", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.0 }, "provenance": { "deposit_id": "8WUV", "doi": "10.1038/s41586-024-07497-8", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8WUV" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 16-nt)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9CGU", "evidence_id": "EV-PDB-9CGU-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.0 }, "provenance": { "deposit_id": "9CGU", "doi": "10.1093/nar/gkae1164", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9CGU" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Cas9 ternary complex, 14-nt sgRNA, State II (kinked)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9EAK", "evidence_id": "EV-PDB-9EAK-4", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.76 }, "provenance": { "deposit_id": "9EAK", "doi": "10.1038/s41467-025-60668-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9EAK" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State I - pre-activation)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9EAL", "evidence_id": "EV-PDB-9EAL-4", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.83 }, "provenance": { "deposit_id": "9EAL", "doi": "10.1038/s41467-025-60668-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9EAL" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State II - precleavage)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9ED9", "evidence_id": "EV-PDB-9ED9-5", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.95 }, "provenance": { "deposit_id": "9ED9", "doi": "10.1038/s41467-025-60668-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9ED9" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State III - checkpoint)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9EDA", "evidence_id": "EV-PDB-9EDA-5", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.88 }, "provenance": { "deposit_id": "9EDA", "doi": "10.1038/s41467-025-60668-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9EDA" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State IV - product)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9H4L", "evidence_id": "EV-PDB-9H4L-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.9 }, "provenance": { "deposit_id": "9H4L", "doi": "10.1038/s41586-026-10255-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9H4L" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "dCas9 bound to off-target 2 EMX1-1 (-)SC DNA minicircle" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9H4M", "evidence_id": "EV-PDB-9H4M-4", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.8 }, "provenance": { "deposit_id": "9H4M", "doi": "10.1038/s41586-026-10255-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9H4M" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "wtCas9 bound to off-target 1 EMX1-1 (-)SC DNA minicircle" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9JL4", "evidence_id": "EV-PDB-9JL4-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 4.0 }, "provenance": { "deposit_id": "9JL4", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9JL4" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Apo-spCas9 conformation" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9K4C", "evidence_id": "EV-PDB-9K4C-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.84 }, "provenance": { "deposit_id": "9K4C", "doi": "10.1038/s41594-026-01753-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9K4C" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SuperFi Cas9 - 22nt sgRNA - DNA ternary complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9K4E", "evidence_id": "EV-PDB-9K4E-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.3 }, "provenance": { "deposit_id": "9K4E", "doi": "10.1038/s41594-026-01753-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9K4E" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SuperFi Cas9 - mismatch 22nt sgRNA - DNA ternary complex class1" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains C)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9LKJ", "evidence_id": "EV-PDB-9LKJ-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.68 }, "provenance": { "deposit_id": "9LKJ", "doi": "10.1038/s41594-025-01741-z", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9LKJ" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Structure of Cas9-sgRNA-A27" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9W7T", "evidence_id": "EV-PDB-9W7T-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.91 }, "provenance": { "deposit_id": "9W7T", "doi": "10.1038/s41594-026-01753-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9W7T" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class B" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9W7U", "evidence_id": "EV-PDB-9W7U-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.83 }, "provenance": { "deposit_id": "9W7U", "doi": "10.1038/s41594-026-01753-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9W7U" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class C" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9W7V", "evidence_id": "EV-PDB-9W7V-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.81 }, "provenance": { "deposit_id": "9W7V", "doi": "10.1038/s41594-026-01753-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9W7V" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class D" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains P)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9WDX", "evidence_id": "EV-PDB-9WDX-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.72 }, "provenance": { "deposit_id": "9WDX", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9WDX" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Structure of SpCas9 with engineered disulfide bonds (R1210C/V1280C and L1119C/P1128C)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9ZQ6", "evidence_id": "EV-PDB-9ZQ6-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.98 }, "provenance": { "deposit_id": "9ZQ6", "doi": "10.1042/BCJ20250364", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9ZQ6" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Structure of SpyCas9 in complex with the anti-CRISPR protein AcrIIA26" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9WAW", "evidence_id": "EV-PDB-9WAW-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.95 }, "provenance": { "deposit_id": "9WAW", "doi": "10.1038/s41594-026-01753-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9WAW" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class E" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9WAA", "evidence_id": "EV-PDB-9WAA-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 4.17 }, "provenance": { "deposit_id": "9WAA", "doi": "10.1038/s41594-026-01753-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9WAA" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SuperFi Cas9 - 22nt sgRNA - DNA ternary complex Class C" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9WA9", "evidence_id": "EV-PDB-9WA9-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 4.12 }, "provenance": { "deposit_id": "9WA9", "doi": "10.1038/s41594-026-01753-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9WA9" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SuperFi Cas9 - 22nt sgRNA - DNA ternary complex Class B" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9W9D", "evidence_id": "EV-PDB-9W9D-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 4.4 }, "provenance": { "deposit_id": "9W9D", "doi": "10.1038/s41594-026-01753-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9W9D" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SuperFi Cas9 - 22nt sgRNA - DNA ternary complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9W7Q", "evidence_id": "EV-PDB-9W7Q-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.79 }, "provenance": { "deposit_id": "9W7Q", "doi": "10.1038/s41594-026-01753-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9W7Q" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class A" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9KJ6", "evidence_id": "EV-PDB-9KJ6-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.59 }, "provenance": { "deposit_id": "9KJ6", "doi": "10.1038/s41598-025-25367-9", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9KJ6" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of SpCas9 ternary complex, amino acids (1242-1263) replaced with Gly-Ser linker" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9K4H", "evidence_id": "EV-PDB-9K4H-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.36 }, "provenance": { "deposit_id": "9K4H", "doi": "10.1038/s41594-026-01753-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9K4H" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SuperFi Cas9 - mismatch 22nt sgRNA - DNA ternary complex class3" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9K4F", "evidence_id": "EV-PDB-9K4F-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.27 }, "provenance": { "deposit_id": "9K4F", "doi": "10.1038/s41594-026-01753-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9K4F" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SuperFi Cas9 - mismatch 22nt sgRNA - DNA ternary complex class2" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9K4D", "evidence_id": "EV-PDB-9K4D-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.53 }, "provenance": { "deposit_id": "9K4D", "doi": "10.1038/s41594-026-01753-3", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9K4D" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SuperFi Cas9 - 20nt sgRNA - DNA ternary complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9JL2", "evidence_id": "EV-PDB-9JL2-3", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.0 }, "provenance": { "deposit_id": "9JL2", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9JL2" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Classical conformation for the spCas9 with gRNA and target DNA complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9JL1", "evidence_id": "EV-PDB-9JL1-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.5 }, "provenance": { "deposit_id": "9JL1", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9JL1" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 with mini-gRNA conformation" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9H4K", "evidence_id": "EV-PDB-9H4K-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.6 }, "provenance": { "deposit_id": "9H4K", "doi": "10.1038/s41586-026-10255-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9H4K" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "dCas9 bound to off-target 1 EMX1-1 (-)SC DNA minicircle" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9H4J", "evidence_id": "EV-PDB-9H4J-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.1 }, "provenance": { "deposit_id": "9H4J", "doi": "10.1038/s41586-026-10255-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9H4J" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "dCas9 bound to on-target EMX1-1 (-)SC DNA minicircle" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9EDB", "evidence_id": "EV-PDB-9EDB-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.1 }, "provenance": { "deposit_id": "9EDB", "doi": "10.1038/s41467-025-60668-7", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9EDB" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 with 17-bp R-loop containing 2 terminal mismatches (State V - dissociated)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9C9P", "evidence_id": "EV-PDB-9C9P-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.1 }, "provenance": { "deposit_id": "9C9P", "doi": "10.1093/nar/gkae1164", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9C9P" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Cas9 ternary complex, 14-nt sgRNA, State I (linear)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains P)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9B2K", "evidence_id": "EV-PDB-9B2K-3", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.67 }, "provenance": { "deposit_id": "9B2K", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9B2K" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9 with dual-guide RNA in open conformation" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains X)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8YNY", "evidence_id": "EV-PDB-8YNY-9", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 4.52 }, "provenance": { "deposit_id": "8YNY", "doi": "10.1038/s41467-024-54768-z", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8YNY" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Structure of Cas9-sgRNA ribonucleoprotein bound to nucleosome" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8YGJ", "evidence_id": "EV-PDB-8YGJ-6", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.2 }, "provenance": { "deposit_id": "8YGJ", "doi": "10.1038/s41586-024-07497-8", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8YGJ" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9-MMLV RT-pegRNA-target DNA complex (elongation 28-nt)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8YE9", "evidence_id": "EV-PDB-8YE9-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.43 }, "provenance": { "deposit_id": "8YE9", "doi": "10.1007/s11427-024-2607-8", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8YE9" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Cryo-EM structure of Cas9-sgRNA-A25 complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8YE6", "evidence_id": "EV-PDB-8YE6-2", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.95 }, "provenance": { "deposit_id": "8YE6", "doi": "10.1007/s11427-024-2607-8", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8YE6" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "Cryo-EM structure of Cas9-sgRNA-A32 complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8WUU", "evidence_id": "EV-PDB-8WUU-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.2 }, "provenance": { "deposit_id": "8WUU", "doi": "10.1038/s41586-024-07497-8", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8WUU" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9-pegRNA-target DNA complex (pre-initiation)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CRISPR-associated endonuclease Cas9/Csn1 (chains A)", "labels": [] }, "data_format": "mmCIF + EMDB map", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8WUT", "evidence_id": "EV-PDB-8WUT-1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 3.0 }, "provenance": { "deposit_id": "8WUT", "doi": "10.1038/s41586-024-07497-8", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8WUT" }, "reader": null, "source_kind": "deposit", "technique": "cryoem", "title": "SpCas9-MMLV RT-pegRNA-target DNA complex (initiation)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "From the first sample condition list of the entry.", "ph": 7.4, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "BMRB data: 13C chemical shifts, 15N chemical shifts, 1H chemical shifts", "labels": [] }, "data_format": "NMR-STAR", "data_level": "processed", "data_link": "https://bmrb.io/data_library/summary/index.php?bmrbId=27949", "evidence_id": "EV-BMRB-27949", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "chemical_shift", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "bmr27949", "doi": "10.13018/BMR27949", "licence": "CC0 1.0 (verify)", "retrieved": "2026-10-19", "source": "BMRB", "url": "https://bmrb.io/data_library/summary/index.php?bmrbId=27949" }, "reader": null, "source_kind": "deposit", "technique": "nmr_shifts", "title": "1H, 13C, 15N Backbone and Sidechain Assignments of HNH from SpyCas9" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_silico", "ions": [], "ligands": [], "notes": "Computed prediction; no experimental conditions.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "AlphaFold Monomer v2.0 pipeline, residues 1-1368", "labels": [] }, "data_format": "mmCIF, PAE JSON", "data_level": "processed", "data_link": "https://alphafold.ebi.ac.uk/entry/Q99ZW2", "evidence_id": "EV-AFDB-AF-Q99ZW2-F1", "mappings": [], "molecule_id": "Q99ZW2", "observable": { "type": "plddt", "uncertainty": null, "unit": "pLDDT", "value": 88.38 }, "provenance": { "deposit_id": "AF-Q99ZW2-F1", "doi": null, "licence": "CC BY 4.0", "retrieved": "2026-10-19", "source": "AlphaFold DB", "url": "https://alphafold.ebi.ac.uk/entry/Q99ZW2" }, "reader": null, "source_kind": "computed", "technique": "af_prediction", "title": "AlphaFold DB prediction AF-Q99ZW2-F1" } ], "models": [], "molecule": { "components": [], "domains": [ { "end": 921, "name": "HNH Cas9-type", "start": 770 } ], "gene": "cas9", "identifiers": { "genbank": null, "isoform": null, "nakb": null, "pdb": [], "rfam": null, "rnacentral": null, "uniprot": "Q99ZW2" }, "ligands": [ "Mn(2+)" ], "modifications": [], "molecule_id": "Q99ZW2", "molecule_type": "protein", "name": "CRISPR-associated endonuclease Cas9/Csn1", "organism": { "name": "Streptococcus pyogenes serotype M1", "taxon_id": 301447 }, "provenance": { "deposit_id": "Q99ZW2", "doi": null, "licence": "CC BY 4.0 (UniProt)", "retrieved": "2026-10-19", "source": "UniProtKB", "url": "https://www.uniprot.org/uniprotkb/Q99ZW2/entry" }, "sequence": "MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD", "sequence_length": 1368 }, "molecule_id": "Q99ZW2", "states": [], "transitions": [] }