{ "conflicts": [], "coverage": { "cells": { "CheY-active": { "af_prediction": 0, "md": 0, "nmr_shifts": 0, "nmr_structure": 0, "xray": 19 }, "CheY-inactive": { "af_prediction": 0, "md": 0, "nmr_shifts": 0, "nmr_structure": 4, "xray": 38 } }, "data_levels": { "CheY-active": { "xray": "processed" }, "CheY-inactive": { "nmr_structure": "processed", "xray": "processed" } }, "single_method_states": [ "CheY-active" ], "states": [ "CheY-inactive", "CheY-active" ], "techniques": [ "xray", "nmr_structure", "nmr_shifts", "af_prediction", "md" ], "unmapped_evidence": 8 }, "curation": { "as_of": "2026-10-19", "curator": "draft (unreviewed)", "file": "P0AE67.yaml", "status": "draft" }, "evidence": [ { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.2, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "CHEMOTAXIS PROTEIN CHEY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1AB6", "evidence_id": "EV-PDB-1AB6-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.2 }, "provenance": { "deposit_id": "1AB6", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1AB6" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF CHEY MUTANT F14N, V86T" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.9, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CHEMOTAXIS PROTEIN CHEY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1C4W", "evidence_id": "EV-PDB-1C4W-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.84 }, "provenance": { "deposit_id": "1C4W", "doi": "10.1021/bi9925524", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1C4W" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "1.9 A STRUCTURE OF A-THIOPHOSPHONATE MODIFIED CHEY D57C" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CHEY (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1CEY", "evidence_id": "EV-PDB-1CEY-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "1CEY", "doi": "10.1021/bi00201a022", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1CEY" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "ASSIGNMENTS, SECONDARY STRUCTURE, GLOBAL FOLD, AND DYNAMICS OF CHEMOTAXIS Y PROTEIN USING THREE-AND FOUR-DIMENSIONAL HETERONUCLEAR (13C,15N) NMR SPECTROSCOPY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CHEY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1CHN", "evidence_id": "EV-PDB-1CHN-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.76 }, "provenance": { "deposit_id": "1CHN", "doi": "10.1006/jmbi.1994.1308", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1CHN" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "MAGNESIUM BINDING TO THE BACTERIAL CHEMOTAXIS PROTEIN CHEY RESULTS IN LARGE CONFORMATIONAL CHANGES INVOLVING ITS FUNCTIONAL SURFACE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 4.8, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CHEMOTAXIS PROTEIN CHEY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1D4Z", "evidence_id": "EV-PDB-1D4Z-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "1D4Z", "doi": "10.1074/jbc.M909908199", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1D4Z" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CRYSTAL STRUCTURE OF CHEY-95IV, A HYPERACTIVE CHEY MUTANT" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 13.85 }, "construct": { "description": "Chemotaxis protein CheY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1E6K", "evidence_id": "EV-PDB-1E6K-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "1E6K", "doi": "10.1006/jmbi.2000.4507", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1E6K" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Two-component signal transduction system D12A mutant of CheY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.3, "salt_mM": null, "temperature_c": 16.85 }, "construct": { "description": "CHEY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EHC", "evidence_id": "EV-PDB-1EHC-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.26 }, "provenance": { "deposit_id": "1EHC", "doi": "10.1074/jbc.272.18.11850", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EHC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF SIGNAL TRANSDUCTION PROTEIN CHEY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "GOL", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.4, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CHEMOTAXIS CHEY PROTEIN (chains A,B,C)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1F4V", "evidence_id": "EV-PDB-1F4V-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.22 }, "provenance": { "deposit_id": "1F4V", "doi": "10.1038/83053", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1F4V" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CRYSTAL STRUCTURE OF ACTIVATED CHEY BOUND TO THE N-TERMINUS OF FLIM" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MN" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.5, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "CHEMOTAXIS PROTEIN CHEY (chains A,C)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1FFG", "evidence_id": "EV-PDB-1FFG-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "1FFG", "doi": "10.1107/S090744490001492X", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1FFG" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY AT 2.1 A RESOLUTION" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MN" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.5, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "CHEMOTAXIS PROTEIN CHEY (chains A,C)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1FFS", "evidence_id": "EV-PDB-1FFS-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.4 }, "provenance": { "deposit_id": "1FFS", "doi": "10.1107/S090744490001492X", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1FFS" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM CRYSTALS SOAKED IN ACETYL PHOSPHATE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MN", "PON" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.5, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "CHEMOTAXIS PROTEIN CHEY (chains A,C)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1FFW", "evidence_id": "EV-PDB-1FFW-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.7 }, "provenance": { "deposit_id": "1FFW", "doi": "10.1107/S090744490001492X", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1FFW" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY WITH A BOUND IMIDO DIPHOSPHATE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "MN" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "CHEMOTAXIS CHEY PROTEIN (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1FQW", "evidence_id": "EV-PDB-1FQW-1", "mappings": [ { "confidence": "high", "justification": "Title: activated CheY; BeF3- (BEF) bound.", "rule": "curator_assignment", "state_id": "CheY-active", "who": "curator:draft (unreviewed)" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.37 }, "provenance": { "deposit_id": "1FQW", "doi": "10.1074/jbc.M101002200", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1FQW" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CRYSTAL STRUCTURE OF ACTIVATED CHEY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "GOL", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.3, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1JBE", "evidence_id": "EV-PDB-1JBE-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.08 }, "provenance": { "deposit_id": "1JBE", "doi": "10.1074/jbc.C100295200", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1JBE" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "1.08 A Structure of apo-Chey reveals meta-active conformation" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BCN", "BEF", "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.5, "salt_mM": null, "temperature_c": -73.15 }, "construct": { "description": "Chemotaxis protein cheY (chains Y)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1KMI", "evidence_id": "EV-PDB-1KMI-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.9 }, "provenance": { "deposit_id": "1KMI", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1KMI" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CRYSTAL STRUCTURE OF AN E.COLI CHEMOTAXIS PROTEIN, CHEZ" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CHEY PROTEIN (chains A,B,C,D)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1UDR", "evidence_id": "EV-PDB-1UDR-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "1UDR", "doi": "10.1006/jmbi.2000.4507", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1UDR" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CHEY MUTANT WITH LYS 91 REPLACED BY ASP, LYS 92 REPLACED BY ALA, ILE 96 REPLACED BY LYS AND ALA 98 REPLACED BY LEU (STABILIZING MUTATIONS IN HELIX 4)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CHEY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1VLZ", "evidence_id": "EV-PDB-1VLZ-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.05 }, "provenance": { "deposit_id": "1VLZ", "doi": "10.1074/jbc.270.29.17386", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1VLZ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "UNCOUPLED PHOSPHORYLATION AND ACTIVATION IN BACTERIAL CHEMOTAXIS: THE 2.1 ANGSTROM STRUCTURE OF A THREONINE TO ISOLEUCINE MUTANT AT POSITION 87 OF CHEY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.2, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "CHEY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1YMU", "evidence_id": "EV-PDB-1YMU-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.3 }, "provenance": { "deposit_id": "1YMU", "doi": "10.1006/jmbi.1996.0151", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1YMU" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SIGNAL TRANSDUCTION PROTEIN CHEY MUTANT WITH MET 17 REPLACED BY GLY (M17G)" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.8, "salt_mM": null, "temperature_c": 3.85 }, "construct": { "description": "CHEY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1YMV", "evidence_id": "EV-PDB-1YMV-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "1YMV", "doi": "10.1006/jmbi.1996.0151", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1YMV" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SIGNAL TRANSDUCTION PROTEIN CHEY MUTANT WITH PHE 14 REPLACED BY GLY, SER 15 REPLACED BY GLY, AND MET 17 REPLACED BY GLY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "Chemotaxis protein cheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2B1J", "evidence_id": "EV-PDB-2B1J-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.4 }, "provenance": { "deposit_id": "2B1J", "doi": "10.1128/JB.00637-06", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2B1J" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of Unphosphorylated CheY Bound to the N-Terminus of FliM" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.84, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein cheY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2ID9", "evidence_id": "EV-PDB-2ID9-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.75 }, "provenance": { "deposit_id": "2ID9", "doi": "10.1016/j.abb.2008.08.019", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2ID9" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "1.85 A Structure of T87I/Y106W Phosphono-CheY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "GOL", "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.4, "salt_mM": null, "temperature_c": null }, "construct": { "description": "Chemotaxis protein cheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3F7N", "evidence_id": "EV-PDB-3F7N-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "3F7N", "doi": "10.1016/j.jmb.2009.07.064", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3F7N" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of CheY triple mutant F14E, N59M, E89L complexed with BeF3- and Mn2+" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "GOL", "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.4, "salt_mM": null, "temperature_c": null }, "construct": { "description": "Chemotaxis protein cheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3FFW", "evidence_id": "EV-PDB-3FFW-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "3FFW", "doi": "10.1016/j.jmb.2009.07.064", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3FFW" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of CheY triple mutant F14Q, N59K, E89Y complexed with BeF3- and Mn2+" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "GOL", "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.4, "salt_mM": null, "temperature_c": null }, "construct": { "description": "Chemotaxis protein cheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3FFX", "evidence_id": "EV-PDB-3FFX-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.01 }, "provenance": { "deposit_id": "3FFX", "doi": "10.1016/j.jmb.2009.07.064", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3FFX" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of CheY triple mutant F14E, N59R, E89H complexed with BeF3- and Mn2+" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "GOL", "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein cheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3MYY", "evidence_id": "EV-PDB-3MYY-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "3MYY", "doi": "10.1021/acs.biochem.1c00246", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3MYY" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of E. Coli CheY mutant A113P bound to Beryllium fluoride" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "MG", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3OLV", "evidence_id": "EV-PDB-3OLV-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.697 }, "provenance": { "deposit_id": "3OLV", "doi": "10.1021/acs.biochem.6b00645", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3OLV" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structural and functional effects of substitution at position T+1 in CheY: CheYA88V-BeF3-Mg complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "CL", "GOL", "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3OLW", "evidence_id": "EV-PDB-3OLW-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.304 }, "provenance": { "deposit_id": "3OLW", "doi": "10.1021/acs.biochem.6b00645", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3OLW" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structural and functional effects of substitution at position T+1 in CheY: CheYA88T-BeF3-Mn complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "GOL", "MG", "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3OLX", "evidence_id": "EV-PDB-3OLX-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "3OLX", "doi": "10.1021/acs.biochem.6b00645", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3OLX" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structural and functional effects of substitution at position T+1 in CheY: CheYA88S-BeF3-Mn complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "GOL", "MN", "NH4", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.25, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3OO0", "evidence_id": "EV-PDB-3OO0-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.55 }, "provenance": { "deposit_id": "3OO0", "doi": "10.1021/acs.biochem.1c00246", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3OO0" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of apo CheY A113P" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3OO1", "evidence_id": "EV-PDB-3OO1-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "3OO1", "doi": "10.1021/acs.biochem.1c00246", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3OO1" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of E. Coli CheY mutant A113P in the absence of Sulfate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MN" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3RVK", "evidence_id": "EV-PDB-3RVK-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.16 }, "provenance": { "deposit_id": "3RVK", "doi": "10.1021/acs.biochem.5b00286", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3RVK" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of the CheY-Mn2+ Complex with substitutions at 59 and 89: N59D E89Q" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "GOL", "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.25, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3RVJ", "evidence_id": "EV-PDB-3RVJ-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "3RVJ", "doi": "10.1021/acs.biochem.5b00286", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3RVJ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89Q" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "GOL", "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3OLY", "evidence_id": "EV-PDB-3OLY-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.05 }, "provenance": { "deposit_id": "3OLY", "doi": "10.1021/acs.biochem.6b00645", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3OLY" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structural and functional effects of substitution at position T+1 in CheY: CheYA88M-BeF3-Mn complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "GOL", "MN", "NH4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.4, "salt_mM": null, "temperature_c": null }, "construct": { "description": "Chemotaxis protein cheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3FGZ", "evidence_id": "EV-PDB-3FGZ-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "3FGZ", "doi": "10.1016/j.jmb.2009.07.064", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3FGZ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of CheY triple mutant F14E, N59M, E89R complexed with BeF3- and Mn2+" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "GOL", "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.4, "salt_mM": null, "temperature_c": null }, "construct": { "description": "Chemotaxis protein cheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3FFT", "evidence_id": "EV-PDB-3FFT-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.21 }, "provenance": { "deposit_id": "3FFT", "doi": "10.1016/j.jmb.2009.07.064", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3FFT" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of CheY double mutant F14E, E89R complexed with BeF3- and Mn2+" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CHEY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3CHY", "evidence_id": "EV-PDB-3CHY-1", "mappings": [ { "confidence": "high", "justification": "Unphosphorylated CheY, 1.7 Å.", "rule": "curator_assignment", "state_id": "CheY-inactive", "who": "curator:draft (unreviewed)" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.66 }, "provenance": { "deposit_id": "3CHY", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3CHY" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CRYSTAL STRUCTURE OF ESCHERICHIA COLI CHEY REFINED AT 1.7-ANGSTROM RESOLUTION" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.5, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "Chemotaxis protein CheY (chains Y)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2LP4", "evidence_id": "EV-PDB-2LP4-2", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "2LP4", "doi": "10.1021/bi300147m", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2LP4" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "Solution structure of P1-CheY/P2 complex in bacterial chemotaxis" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "ACT" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.88, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein cheY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2IDM", "evidence_id": "EV-PDB-2IDM-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "2IDM", "doi": "10.1016/j.abb.2008.08.019", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2IDM" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "2.00 A Structure of T87I/Y106W Phosphono-CheY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 4.6, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein cheY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2ID7", "evidence_id": "EV-PDB-2ID7-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.75 }, "provenance": { "deposit_id": "2ID7", "doi": "10.1016/j.abb.2008.08.019", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2ID7" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "1.75 A Structure of T87I Phosphono-CheY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "MN", "XE" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.4, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein cheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1ZDM", "evidence_id": "EV-PDB-1ZDM-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.4 }, "provenance": { "deposit_id": "1ZDM", "doi": "10.1110/ps.041231005", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1ZDM" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of Activated CheY Bound to Xe" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.9, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein cheY (chains A,B,C,D)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1U8T", "evidence_id": "EV-PDB-1U8T-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.5 }, "provenance": { "deposit_id": "1U8T", "doi": "10.1016/j.jmb.2004.07.084", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1U8T" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.4, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein cheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1MIH", "evidence_id": "EV-PDB-1MIH-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.7 }, "provenance": { "deposit_id": "1MIH", "doi": "10.1128/JB.185.5.1495-1502.2003", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1MIH" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "A ROLE FOR CHEY GLU 89 IN CHEZ-MEDIATED DEPHOSPHORYLATION OF THE E. COLI CHEMOTAXIS RESPONSE REGULATOR CHEY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CHEY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1HEY", "evidence_id": "EV-PDB-1HEY-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.24 }, "provenance": { "deposit_id": "1HEY", "doi": "10.1006/jmbi.1995.0326", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1HEY" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "INVESTIGATING THE STRUCTURAL DETERMINANTS OF THE P21-LIKE TRIPHOSPHATE AND MG2+ BINDING SITE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 13.85 }, "construct": { "description": "CHEY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EAY", "evidence_id": "EV-PDB-1EAY-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "1EAY", "doi": "10.1073/pnas.95.13.7333", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EAY" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CHEY-BINDING (P2) DOMAIN OF CHEA IN COMPLEX WITH CHEY FROM ESCHERICHIA COLI" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 13.85 }, "construct": { "description": "CHEMOTAXIS PROTEIN CHEY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1E6M", "evidence_id": "EV-PDB-1E6M-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "1E6M", "doi": "10.1006/jmbi.2000.4507", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1E6M" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "TWO-COMPONENT SIGNAL TRANSDUCTION SYSTEM D57A MUTANT OF CHEY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 13.85 }, "construct": { "description": "Chemotaxis protein CheY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1E6L", "evidence_id": "EV-PDB-1E6L-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "1E6L", "doi": "10.1006/jmbi.2000.4507", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1E6L" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Two-component signal transduction system D13A mutant of CheY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.7, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "CHEMOTAXIS PROTEIN Y (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1DJM", "evidence_id": "EV-PDB-1DJM-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "1DJM", "doi": "10.1006/jmbi.2000.3595", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1DJM" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "SOLUTION STRUCTURE OF BEF3-ACTIVATED CHEY FROM ESCHERICHIA COLI" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "CHEY (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1CYE", "evidence_id": "EV-PDB-1CYE-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "1CYE", "doi": "10.1016/S0022-2836(05)80150-0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1CYE" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "THREE DIMENSIONAL STRUCTURE OF CHEMOTACTIC CHE Y PROTEIN IN AQUEOUS SOLUTION BY NUCLEAR MAGNETIC RESONANCE METHODS" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 9.85 }, "construct": { "description": "CHEY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1BDJ", "evidence_id": "EV-PDB-1BDJ-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.68 }, "provenance": { "deposit_id": "1BDJ", "doi": "10.1107/S0907444999005053", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1BDJ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "COMPLEX STRUCTURE OF HPT DOMAIN AND CHEY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.2, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "CHEY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1AB5", "evidence_id": "EV-PDB-1AB5-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.4 }, "provenance": { "deposit_id": "1AB5", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1AB5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF CHEY MUTANT F14N, V21T" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MN" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "CHEY (chains A,C,E,G)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1A0O", "evidence_id": "EV-PDB-1A0O-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.95 }, "provenance": { "deposit_id": "1A0O", "doi": "10.1038/nsb0198-25", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1A0O" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "GOL", "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.25, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3RVL", "evidence_id": "EV-PDB-3RVL-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.55 }, "provenance": { "deposit_id": "3RVL", "doi": "10.1021/acs.biochem.5b00286", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3RVL" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89R" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MN" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.25, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3RVM", "evidence_id": "EV-PDB-3RVM-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.45 }, "provenance": { "deposit_id": "3RVM", "doi": "10.1021/acs.biochem.5b00286", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3RVM" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of the CheY-Mn2+ Complex with substitutions at 59 and 89: N59D and E89R" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "GOL", "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.25, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3RVN", "evidence_id": "EV-PDB-3RVN-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.25 }, "provenance": { "deposit_id": "3RVN", "doi": "10.1021/acs.biochem.5b00286", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3RVN" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89Y" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MN" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3RVO", "evidence_id": "EV-PDB-3RVO-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.55 }, "provenance": { "deposit_id": "3RVO", "doi": "10.1021/acs.biochem.5b00286", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3RVO" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of CheY-Mn2+ Complex with substitutions at 59 and 89: N59D E89Y" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "BEF", "GOL", "MG", "MN", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3RVP", "evidence_id": "EV-PDB-3RVP-1", "mappings": [ { "confidence": "high", "justification": "BeF3- (BEF) bound: phosphoryl mimic that activates CheY.", "rule": "chey-bef3-active", "state_id": "CheY-active", "who": "rule:chey-bef3-active" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.404 }, "provenance": { "deposit_id": "3RVP", "doi": "10.1021/acs.biochem.5b00286", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3RVP" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of the CheY-BeF3 Complex with substitutions at 59 and 89: N59D and E89K" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MN" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3RVQ", "evidence_id": "EV-PDB-3RVQ-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.15 }, "provenance": { "deposit_id": "3RVQ", "doi": "10.1021/acs.biochem.5b00286", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3RVQ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of the CheY-Mn2+ Complex with substitutions at 59 and 89: N59D E89K" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "GOL", "MN", "MOO", "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3RVR", "evidence_id": "EV-PDB-3RVR-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "3RVR", "doi": "10.1021/acs.biochem.5b00286", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3RVR" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of the CheYN59D/E89R Molybdate complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": 18.85 }, "construct": { "description": "CHEY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5CHY", "evidence_id": "EV-PDB-5CHY-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "5CHY", "doi": "10.1074/jbc.272.38.23758", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5CHY" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF CHEMOTAXIS PROTEIN CHEY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "SO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": -193.15 }, "construct": { "description": "CHEY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6CHY", "evidence_id": "EV-PDB-6CHY-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.33 }, "provenance": { "deposit_id": "6CHY", "doi": "10.1074/jbc.272.38.23758", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6CHY" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF CHEMOTAXIS PROTEIN CHEY" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MG" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6TG7", "evidence_id": "EV-PDB-6TG7-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "6TG7", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6TG7" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of the CheY in presence of magnesium" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "GOL", "MN", "SO4", "WO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Chemotaxis protein CheY (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3RVS", "evidence_id": "EV-PDB-3RVS-1", "mappings": [ { "confidence": "low", "justification": "Structure without a phosphoryl mimic; usually inactive, but mutants can be partly active.", "rule": "chey-unphosphorylated", "state_id": "CheY-inactive", "who": "rule:chey-unphosphorylated" } ], "molecule_id": "P0AE67", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "3RVS", "doi": "10.1021/acs.biochem.5b00286", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3RVS" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of the CheYN59D/E89R Tungstate complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "From the first sample condition list of the entry.", "ph": 6.5, "salt_mM": null, "temperature_c": -0.15 }, "construct": { "description": "BMRB data: 15N chemical shifts, 1H chemical shifts", "labels": [] }, "data_format": "NMR-STAR", "data_level": "processed", "data_link": "https://bmrb.io/data_library/summary/index.php?bmrbId=18234", "evidence_id": "EV-BMRB-18234", "mappings": [], "molecule_id": "P0AE67", "observable": { "type": "chemical_shift", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "bmr18234", "doi": "10.13018/BMR18234", "licence": "CC0 1.0 (verify)", "retrieved": "2026-10-19", "source": "BMRB", "url": "https://bmrb.io/data_library/summary/index.php?bmrbId=18234" }, "reader": null, "source_kind": "deposit", "technique": "nmr_shifts", "title": "Solution structure of P1-CheY/P2 complex in bacterial chemotaxis" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "From the first sample condition list of the entry.", "ph": 6.4, "salt_mM": null, "temperature_c": 29.85 }, "construct": { "description": "BMRB data: 1H chemical shifts", "labels": [] }, "data_format": "NMR-STAR", "data_level": "processed", "data_link": "https://bmrb.io/data_library/summary/index.php?bmrbId=2950", "evidence_id": "EV-BMRB-2950", "mappings": [], "molecule_id": "P0AE67", "observable": { "type": "chemical_shift", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "bmr2950", "doi": "10.13018/BMR2950", "licence": "CC0 1.0 (verify)", "retrieved": "2026-10-19", "source": "BMRB", "url": "https://bmrb.io/data_library/summary/index.php?bmrbId=2950" }, "reader": null, "source_kind": "deposit", "technique": "nmr_shifts", "title": "Assessment of cheY binding regions using 2D NMR and paramagnetic ligands" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "From the first sample condition list of the entry.", "ph": 6.4, "salt_mM": null, "temperature_c": 29.85 }, "construct": { "description": "BMRB data: 1H chemical shifts", "labels": [] }, "data_format": "NMR-STAR", "data_level": "processed", "data_link": "https://bmrb.io/data_library/summary/index.php?bmrbId=2951", "evidence_id": "EV-BMRB-2951", "mappings": [], "molecule_id": "P0AE67", "observable": { "type": "chemical_shift", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "bmr2951", "doi": "10.13018/BMR2951", "licence": "CC0 1.0 (verify)", "retrieved": "2026-10-19", "source": "BMRB", "url": "https://bmrb.io/data_library/summary/index.php?bmrbId=2951" }, "reader": null, "source_kind": "deposit", "technique": "nmr_shifts", "title": "Assessment of cheY binding regions using 2D NMR and paramagnetic ligands" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "From the first sample condition list of the entry.", "ph": 7.0, "salt_mM": null, "temperature_c": 34.85 }, "construct": { "description": "BMRB data: 15N chemical shifts, 1H chemical shifts", "labels": [] }, "data_format": "NMR-STAR", "data_level": "processed", "data_link": "https://bmrb.io/data_library/summary/index.php?bmrbId=3440", "evidence_id": "EV-BMRB-3440", "mappings": [], "molecule_id": "P0AE67", "observable": { "type": "chemical_shift", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "bmr3440", "doi": "10.13018/BMR3440", "licence": "CC0 1.0 (verify)", "retrieved": "2026-10-19", "source": "BMRB", "url": "https://bmrb.io/data_library/summary/index.php?bmrbId=3440" }, "reader": null, "source_kind": "deposit", "technique": "nmr_shifts", "title": "1H- and 15N-NMR assignment and solution structure of the chemotactic Escherichia coli Che Y protein" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "From the first sample condition list of the entry.", "ph": 6.75, "salt_mM": null, "temperature_c": 29.85 }, 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"N6-acetyllysine", "position": 92, "source": "UniProtKB" }, { "kind": "ptm", "name": "N6-acetyllysine", "position": 109, "source": "UniProtKB" } ], "molecule_id": "P0AE67", "molecule_type": "protein", "name": "Chemotaxis protein CheY", "organism": { "name": "Escherichia coli (strain K12)", "taxon_id": 83333 }, "provenance": { "deposit_id": "P0AE67", "doi": null, "licence": "CC BY 4.0 (UniProt)", "retrieved": "2026-10-19", "source": "UniProtKB", "url": "https://www.uniprot.org/uniprotkb/P0AE67/entry" }, "sequence": "MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM", "sequence_length": 129 }, "molecule_id": "P0AE67", "states": [ { "assumptions": [], "basis": "observed", "description": "Unphosphorylated receiver domain, as in 3CHY.", "family_label": null, "molecule_id": "P0AE67", "name": "inactive (unphosphorylated)", "parent_state": null, "population": null, "provenance": { "deposit_id": null, "doi": null, 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