{ "conflicts": [], "coverage": { "cells": { "SNase-apo": { "af_prediction": 0, "force_spectroscopy": 0, "nmr_shifts": 0, "nmr_structure": 9, "xray": 87 }, "SNase-pdTp": { "af_prediction": 0, "force_spectroscopy": 0, "nmr_shifts": 0, "nmr_structure": 2, "xray": 197 }, "SNase-unfolded": { "af_prediction": 0, "force_spectroscopy": 0, "nmr_shifts": 6, "nmr_structure": 0, "xray": 0 } }, "data_levels": { "SNase-apo": { "nmr_structure": "processed", "xray": "processed" }, "SNase-pdTp": { "nmr_structure": "processed", "xray": "processed" }, "SNase-unfolded": { "nmr_shifts": "processed" } }, "single_method_states": [ "SNase-unfolded" ], "states": [ "SNase-apo", "SNase-pdTp", "SNase-unfolded" ], "techniques": [ "xray", "nmr_structure", "nmr_shifts", "force_spectroscopy", "af_prediction" ], "unmapped_evidence": 34 }, "curation": { "as_of": "2026-10-19", "curator": "draft (unreviewed)", "file": "P00644.yaml", "status": "draft" }, "evidence": [ { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 5.0 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1A2T", "evidence_id": "EV-PDB-1A2T-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.96 }, "provenance": { "deposit_id": "1A2T", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1A2T" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STAPHYLOCOCCAL NUCLEASE, B-MERCAPTOETHANOL DISULFIDE TO V23C VARIANT" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 5.0 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1A2U", "evidence_id": "EV-PDB-1A2U-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "1A2U", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1A2U" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 1-N-BUTANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 5.0 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1A3U", "evidence_id": "EV-PDB-1A3U-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.05 }, "provenance": { "deposit_id": "1A3U", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1A3U" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STAPHYLOCOCCAL NUCLEASE, CYCLOHEXANE THIOL DISULFIDE TO V23C VARIANT" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 5.0 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1A3V", "evidence_id": "EV-PDB-1A3V-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.2 }, "provenance": { "deposit_id": "1A3V", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1A3V" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STAPHYLOCOCCAL NUCLEASE, CYCLOPENTANE THIOL DISULFIDE TO V23C VARIANT" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1ENA", "evidence_id": "EV-PDB-1ENA-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.15 }, "provenance": { "deposit_id": "1ENA", "doi": "10.1021/bi00192a004", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1ENA" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EY0", "evidence_id": "EV-PDB-1EY0-1", "mappings": [ { "confidence": "high", "justification": "Wild-type, ligand-free structure; representative of the apo state.", "rule": "curator_assignment", "state_id": "SNase-apo", "who": "curator:draft (unreviewed)" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "1EY0", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EY0" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF WILD-TYPE S. NUCLEASE AT 1.6 A RESOLUTION" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EY4", "evidence_id": "EV-PDB-1EY4-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "1EY4", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EY4" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF S. NUCLEASE STABILIZING MUTANT S59A" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EY5", "evidence_id": "EV-PDB-1EY5-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "1EY5", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EY5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T33V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EY6", "evidence_id": "EV-PDB-1EY6-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.75 }, "provenance": { "deposit_id": "1EY6", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EY6" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T41I" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EY7", "evidence_id": "EV-PDB-1EY7-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.88 }, "provenance": { "deposit_id": "1EY7", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EY7" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF S. NUCLEASE STABILIZING MUTANT S128A" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EY8", "evidence_id": "EV-PDB-1EY8-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.75 }, "provenance": { "deposit_id": "1EY8", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EY8" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF S. NUCLEASE STABILIZING TRIPLE MUTANT P117G/H124L/S128A" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EY9", "evidence_id": "EV-PDB-1EY9-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.72 }, "provenance": { "deposit_id": "1EY9", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EY9" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF S. NUCLEASE STABILIZING QUADRUPLE MUTANT T41I/P117G/H124L/S128A" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EYA", "evidence_id": "EV-PDB-1EYA-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "1EYA", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EYA" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T33V/T41I/P117G/H124L/S128A" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EYC", "evidence_id": "EV-PDB-1EYC-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "1EYC", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EYC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF S. NUCLEASE STABILIZING QUINTUPLE MUTANT T41I/S59A/P117G/H124L/S128A" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EYD", "evidence_id": "EV-PDB-1EYD-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "1EYD", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EYD" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF WILD-TYPE S. NUCLEASE AT 1.7 A RESOLUTION" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EZ6", "evidence_id": "EV-PDB-1EZ6-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "1EZ6", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EZ6" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF S. NUCLEASE STABILIZING SEXTUPLE MUTANT T33V/T41I/S59A/P117G/H124L/S128A" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EZ8", "evidence_id": "EV-PDB-1EZ8-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "1EZ8", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EZ8" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRUCTURE OF S. NUCLEASE STABILIZING MUTANT T33V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.5, "salt_mM": null, "temperature_c": 44.85 }, "construct": { "description": "staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1JOQ", "evidence_id": "EV-PDB-1JOQ-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "1JOQ", "doi": "10.1023/A:1018350004729", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1JOQ" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "Ensemble structures for Staphylococcal nuclease-H124L in ternary complex with Ca2+ and thymidine-3',5'-bisphosphate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1KAA", "evidence_id": "EV-PDB-1KAA-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "1KAA", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1KAA" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRESS AND STRAIN IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1KAB", "evidence_id": "EV-PDB-1KAB-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "1KAB", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1KAB" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STRESS AND STRAIN IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1KDA", "evidence_id": "EV-PDB-1KDA-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "1KDA", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1KDA" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1KDB", "evidence_id": "EV-PDB-1KDB-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "1KDB", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1KDB" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 5.0 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1NUC", "evidence_id": "EV-PDB-1NUC-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "1NUC", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1NUC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STAPHYLOCOCCAL NUCLEASE, V23C VARIANT" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 4.9, "salt_mM": null, "temperature_c": 31.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1RKN", "evidence_id": "EV-PDB-1RKN-1", "mappings": [], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "1RKN", "doi": "10.1529/biophysj.106.092155", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1RKN" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "Solution structure of 1-110 fragment of Staphylococcal Nuclease with G88W mutation" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "THERMONUCLEASE PRECURSOR (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1SNC", "evidence_id": "EV-PDB-1SNC-1", "mappings": [ { "confidence": "high", "justification": "Ca2+ and pdTp ternary complex; representative of the inhibitor-bound state.", "rule": "curator_assignment", "state_id": "SNase-pdTp", "who": "curator:draft (unreviewed)" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "1SNC", "doi": "10.1002/prot.340050302", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1SNC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "THE CRYSTAL STRUCTURE OF THE TERNARY COMPLEX OF STAPHYLOCOCCAL NUCLEASE, CA2+, AND THE INHIBITOR PD*TP, REFINED AT 1.65 ANGSTROMS" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE DIMER (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1SND", "evidence_id": "EV-PDB-1SND-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.84 }, "provenance": { "deposit_id": "1SND", "doi": "10.1038/nsb0995-746", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1SND" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STAPHYLOCOCCAL NUCLEASE DIMER CONTAINING A DELETION OF RESIDUES 114-119 COMPLEXED WITH CALCIUM CHLORIDE AND THE COMPETITIVE INHIBITOR DEOXYTHYMIDINE-3',5'-DIPHOSPHATE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "THERMONUCLEASE PRECURSOR (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1SNM", "evidence_id": "EV-PDB-1SNM-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.74 }, "provenance": { "deposit_id": "1SNM", "doi": "10.1021/bi00481a016", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1SNM" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "ACTIVE SITE MUTANT GLU-43 (RIGHT ARROW) ASP IN STAPHYLOCOCCAL NUCLEASE DISPLAYS NONLOCAL STRUCTURAL CHANGES" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 3.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1SNO", "evidence_id": "EV-PDB-1SNO-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "1SNO", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1SNO" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 3.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1SNP", "evidence_id": "EV-PDB-1SNP-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.95 }, "provenance": { "deposit_id": "1SNP", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1SNP" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 3.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1SNQ", "evidence_id": "EV-PDB-1SNQ-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "1SNQ", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1SNQ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "PROTEIN STABILITY IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1STA", "evidence_id": "EV-PDB-1STA-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "1STA", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1STA" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CO", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1STG", "evidence_id": "EV-PDB-1STG-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "1STG", "doi": "10.1021/bi00013a021", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1STG" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CO", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1STH", "evidence_id": "EV-PDB-1STH-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "1STH", "doi": "10.1021/bi00013a021", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1STH" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "TWO DISTINCTLY DIFFERENT METAL BINDING MODES ARE SEEN IN X-RAY CRYSTAL STRUCTURES OF STAPHYLOCOCCAL NUCLEASE-COBALT(II)-NUCLEOTIDE COMPLEXES" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1STN", "evidence_id": "EV-PDB-1STN-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "1STN", "doi": "10.1002/prot.340100203", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1STN" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "THE CRYSTAL STRUCTURE OF STAPHYLOCOCCAL NUCLEASE REFINED AT 1.7 ANGSTROMS RESOLUTION" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1STY", "evidence_id": "EV-PDB-1STY-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.67 }, "provenance": { "deposit_id": "1STY", "doi": "10.1073/pnas.90.8.3275", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1STY" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "THE ALPHA ANEURISM: A STRUCTURAL MOTIF REVEALED IN AN INSERTION MUTANT OF STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1STB", "evidence_id": "EV-PDB-1STB-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "1STB", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1STB" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "ACCOMMODATION OF INSERTION MUTATIONS ON THE SURFACE AND IN THE INTERIOR OF STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains S)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1NSN", "evidence_id": "EV-PDB-1NSN-3", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.8 }, "provenance": { "deposit_id": "1NSN", "doi": "10.1006/jmbi.1995.0573", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1NSN" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "THE CRYSTAL STRUCTURE OF ANTIBODY N10-STAPHYLOCOCCAL NUCLEASE COMPLEX AT 2.9 ANGSTROMS RESOLUTION" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1KDC", "evidence_id": "EV-PDB-1KDC-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "1KDC", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1KDC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STABILIZATION OF A STRAINED PROTEIN LOOP CONFORMATION THROUGH PROTEIN ENGINEERING" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.5, "salt_mM": null, "temperature_c": 44.85 }, "construct": { "description": "staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1JOR", "evidence_id": "EV-PDB-1JOR-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "1JOR", "doi": "10.1023/A:1018350004729", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1JOR" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "Ensemble structures for unligated Staphylococcal nuclease-H124L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.5, "salt_mM": null, "temperature_c": 44.85 }, "construct": { "description": "staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1JOO", "evidence_id": "EV-PDB-1JOO-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "1JOO", "doi": "10.1023/A:1018350004729", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1JOO" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "Averaged structure for unligated Staphylococcal nuclease-H124L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.5, "salt_mM": null, "temperature_c": 44.85 }, "construct": { "description": "staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1JOK", "evidence_id": "EV-PDB-1JOK-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "1JOK", "doi": "10.1023/A:1018350004729", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1JOK" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "Averaged structure for Staphylococcal nuclease-H124L in ternary complex with Ca2+ and thymidine-3',5'-bisphosphate" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1II3", "evidence_id": "EV-PDB-1II3-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.72 }, "provenance": { "deposit_id": "1II3", "doi": "10.1016/j.bpc.2004.02.007", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1II3" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of S. nuclease quintuple mutant V23I/V66L/I72L/I92L/V99L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": 23.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1IHZ", "evidence_id": "EV-PDB-1IHZ-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "1IHZ", "doi": "10.1016/j.bpc.2004.02.007", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1IHZ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Structure of S. nuclease mutant quintuple mutant V23L/V66L/I72L/I92L/V99L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 4.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1F2Z", "evidence_id": "EV-PDB-1F2Z-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "1F2Z", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1F2Z" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 4.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1F2Y", "evidence_id": "EV-PDB-1F2Y-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "1F2Y", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1F2Y" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 4.85 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1F2M", "evidence_id": "EV-PDB-1F2M-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "1F2M", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1F2M" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 4.85 }, "construct": { "description": "THERMONUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1EQV", "evidence_id": "EV-PDB-1EQV-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.94 }, "provenance": { "deposit_id": "1EQV", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1EQV" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "SIMPLIFICATION OF A PROTEIN LOOP IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1ENC", "evidence_id": "EV-PDB-1ENC-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.95 }, "provenance": { "deposit_id": "1ENC", "doi": "10.1021/bi00192a004", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1ENC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 5.0 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1AEX", "evidence_id": "EV-PDB-1AEX-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "1AEX", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1AEX" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STAPHYLOCOCCAL NUCLEASE, METHANE THIOL DISULFIDE TO V23C VARIANT" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 5.0 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1A3T", "evidence_id": "EV-PDB-1A3T-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "1A3T", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1A3T" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STAPHYLOCOCCAL NUCLEASE, V23C VARIANT, COMPLEX WITH 2-FLUOROETHANE THIOL AND 3',5'-THYMIDINE DIPHOSPHATE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -178.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1TQO", "evidence_id": "EV-PDB-1TQO-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "1TQO", "doi": "10.1016/j.jmb.2004.05.066", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1TQO" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Cryogenic Crystal Structure of Staphylococcal Nuclease Variant truncated Delta+PHS I92E" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1TR5", "evidence_id": "EV-PDB-1TR5-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "1TR5", "doi": "10.1016/j.jmb.2004.05.066", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1TR5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Room temperature structure of Staphylococcal nuclease variant truncated Delta+PHS I92E" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2ENB", "evidence_id": "EV-PDB-2ENB-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.05 }, "provenance": { "deposit_id": "2ENB", "doi": "10.1021/bi00192a004", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2ENB" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "CRYSTAL STRUCTURES OF THE BINARY CA2+ AND PDTP COMPLEXES AND THE TERNARY COMPLEX OF THE ASP 21->GLU MUTANT OF STAPHYLOCOCCAL NUCLEASE. IMPLICATIONS FOR CATALYSIS AND LIGAND BINDING" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2EXZ", "evidence_id": "EV-PDB-2EXZ-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "2EXZ", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2EXZ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant T22C" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2EY1", "evidence_id": "EV-PDB-2EY1-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "2EY1", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2EY1" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant T22V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2EY2", "evidence_id": "EV-PDB-2EY2-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "2EY2", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2EY2" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant T41C" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2EY6", "evidence_id": "EV-PDB-2EY6-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "2EY6", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2EY6" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant T41V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2EYJ", "evidence_id": "EV-PDB-2EYJ-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "2EYJ", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2EYJ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant T62V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2EYM", "evidence_id": "EV-PDB-2EYM-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "2EYM", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2EYM" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant T120C" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2EYP", "evidence_id": "EV-PDB-2EYP-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "2EYP", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2EYP" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant T120V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0D", "evidence_id": "EV-PDB-2F0D-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.5 }, "provenance": { "deposit_id": "2F0D", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0D" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant I92V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0E", "evidence_id": "EV-PDB-2F0E-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.95 }, "provenance": { "deposit_id": "2F0E", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0E" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant V23L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0G", "evidence_id": "EV-PDB-2F0G-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "2F0G", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0G" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant V66I" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": null }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0I", "evidence_id": "EV-PDB-2F0I-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "2F0I", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0I" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant I72L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0J", "evidence_id": "EV-PDB-2F0J-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "2F0J", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0J" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant I72V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0L", "evidence_id": "EV-PDB-2F0L-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "2F0L", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0L" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant V23L/I72L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0M", "evidence_id": "EV-PDB-2F0M-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "2F0M", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0M" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant V23L/I72V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0O", "evidence_id": "EV-PDB-2F0O-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "2F0O", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0O" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant V66I/I72V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; 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oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0S", "evidence_id": "EV-PDB-2F0S-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.75 }, "provenance": { "deposit_id": "2F0S", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0S" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant V66L/I92V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0T", "evidence_id": "EV-PDB-2F0T-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "2F0T", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0T" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant V66L/V99I" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0U", "evidence_id": "EV-PDB-2F0U-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.83 }, "provenance": { "deposit_id": "2F0U", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0U" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant V23I/L25I/I72V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0V", "evidence_id": "EV-PDB-2F0V-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "2F0V", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0V" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant V23L/V66L/I72L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0W", "evidence_id": "EV-PDB-2F0W-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "2F0W", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0W" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant V23I/L25I/V66L/I72L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 4.9, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F3V", "evidence_id": "EV-PDB-2F3V-1", "mappings": [], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "2F3V", "doi": "10.1529/biophysj.106.092155", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F3V" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "Solution structure of 1-110 fragment of staphylococcal nuclease with V66W mutation" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F3W", "evidence_id": "EV-PDB-2F3W-1", "mappings": [], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "2F3W", "doi": "10.1529/biophysj.106.092155", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F3W" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "solution structure of 1-110 fragment of staphylococcal nuclease in 2M TMAO" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.0, "salt_mM": null, "temperature_c": 26.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2KHS", "evidence_id": "EV-PDB-2KHS-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "2KHS", "doi": "10.1021/bi901099s", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2KHS" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "Solution structure of SNase121:SNase(111-143) complex" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 5.0 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2NUC", "evidence_id": "EV-PDB-2NUC-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "2NUC", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2NUC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STAPHLOCOCCAL NUCLEASE, ETHANE THIOL DISULFIDE TO V23C VARIANT" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -178.15 }, "construct": { "description": "Staphylococcal thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2OEO", "evidence_id": "EV-PDB-2OEO-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "2OEO", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2OEO" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Cryogenic crystal structure of Staphylococcal Nuclease variant truncated Delta+PHS I92D" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "NA" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains W,X,Y,Z)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2GSI", "evidence_id": "EV-PDB-2GSI-3", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.81 }, "provenance": { "deposit_id": "2GSI", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2GSI" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of a Murine Fab in Complex with an 11 Residue Peptide Derived from Staphylococcal Nuclease" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.0, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "13-mer peptide from Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2FXZ", "evidence_id": "EV-PDB-2FXZ-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "2FXZ", "doi": "10.1002/bip.20558", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2FXZ" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "Solution structure of 97-109 segment of staphylococcal nuclease" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.0, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "18-mer peptide from Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2FXY", "evidence_id": "EV-PDB-2FXY-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "2FXY", "doi": "10.1002/bip.20558", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2FXY" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "Solution structure of 55-72 segment of staphylococcal nuclease" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0Q", "evidence_id": "EV-PDB-2F0Q-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.95 }, "provenance": { "deposit_id": "2F0Q", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0Q" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant V66L/I92L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0N", "evidence_id": "EV-PDB-2F0N-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "2F0N", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0N" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant L25I/I72L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0K", "evidence_id": "EV-PDB-2F0K-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "2F0K", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0K" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant V23I/L25I" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0H", "evidence_id": "EV-PDB-2F0H-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.15 }, "provenance": { "deposit_id": "2F0H", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0H" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant V66L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2F0F", "evidence_id": "EV-PDB-2F0F-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.15 }, "provenance": { "deposit_id": "2F0F", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2F0F" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant L25I" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2EYO", "evidence_id": "EV-PDB-2EYO-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "2EYO", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2EYO" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant T120S" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2EYL", "evidence_id": "EV-PDB-2EYL-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "2EYL", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2EYL" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant T82S" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2EYH", "evidence_id": "EV-PDB-2EYH-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "2EYH", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2EYH" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant T62S" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2EYF", "evidence_id": "EV-PDB-2EYF-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "2EYF", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2EYF" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant T44V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Staphylococcal nuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2EY5", "evidence_id": "EV-PDB-2EY5-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "2EY5", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2EY5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease mutant T41S" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.4, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1U9R", "evidence_id": "EV-PDB-1U9R-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "1U9R", "doi": "10.1529/biophysj.104.048454", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1U9R" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of Staphylococcal Nuclease mutant V66E/P117G/H124L/S128A at Room Temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "DMS", "GOL", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.1, "salt_mM": null, "temperature_c": -178.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1TT2", "evidence_id": "EV-PDB-1TT2-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "1TT2", "doi": "10.1016/j.jmb.2004.05.066", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1TT2" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Cryogenic crystal structure of Staphylococcal nuclease variant truncated Delta+PHS I92K" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1SYG", "evidence_id": "EV-PDB-1SYG-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "1SYG", "doi": "10.1021/bi00183a004", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1SYG" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1SYF", "evidence_id": "EV-PDB-1SYF-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "1SYF", "doi": "10.1021/bi00183a004", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1SYF" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1SYE", "evidence_id": "EV-PDB-1SYE-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "1SYE", "doi": "10.1021/bi00183a004", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1SYE" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1SYD", "evidence_id": "EV-PDB-1SYD-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "1SYD", "doi": "10.1021/bi00183a004", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1SYD" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1SYC", "evidence_id": "EV-PDB-1SYC-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "1SYC", "doi": "10.1021/bi00183a004", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1SYC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "ENGINEERING ALTERNATIVE BETA-TURN TYPES IN STAPHYLOCOCCAL NUCLEASE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/1SYB", "evidence_id": "EV-PDB-1SYB-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "1SYB", "doi": "10.1038/339073a0", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/1SYB" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "TRANSFER OF A BETA-TURN STRUCTURE TO A NEW PROTEIN CONTEXT" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "DMS", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -175.15 }, "construct": { "description": "Staphylococcal thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2OF1", "evidence_id": "EV-PDB-2OF1-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.92 }, "provenance": { "deposit_id": "2OF1", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2OF1" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Cryogenic crystal structure of the Staphylococcal Nuclease variant truncated Delta+PHS I92W" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 5.0, "salt_mM": null, "temperature_c": 26.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2PQE", "evidence_id": "EV-PDB-2PQE-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "2PQE", "doi": "10.1021/bi7009794", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2PQE" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "Solution structure of proline-free mutant of staphylococcal nuclease" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "PO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2QDB", "evidence_id": "EV-PDB-2QDB-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.2 }, "provenance": { "deposit_id": "2QDB", "doi": "10.1016/j.jmb.2008.04.021", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2QDB" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of staphylococcal nuclease variant E75Q/D21N/T33V/T41I/S59A/P117G/S128A at 100 K" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "PO4", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2RBM", "evidence_id": "EV-PDB-2RBM-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "2RBM", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2RBM" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "PO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2RDF", "evidence_id": "EV-PDB-2RDF-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.01 }, "provenance": { "deposit_id": "2RDF", "doi": "10.1016/j.jmb.2008.04.021", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2RDF" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of staphyloccocal nuclease VIAGAN/E75A variant at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "PO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 10.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2RKS", "evidence_id": "EV-PDB-2RKS-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.01 }, "provenance": { "deposit_id": "2RKS", "doi": "10.1110/ps.073397708", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2RKS" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant PHS L38K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2SNM", "evidence_id": "EV-PDB-2SNM-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.97 }, "provenance": { "deposit_id": "2SNM", "doi": "10.1016/0022-2836(91)80195-Z", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2SNM" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "IN A STAPHYLOCOCCAL NUCLEASE MUTANT THE SIDE-CHAIN of A LYSINE REPLACING VALINE 66 IS FULLY BURIED IN THE HYDROPHOBIC CORE" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "THERMONUCLEASE PRECURSOR (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2SNS", "evidence_id": "EV-PDB-2SNS-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.5 }, "provenance": { "deposit_id": "2SNS", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2SNS" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STAPHYLOCOCCAL NUCLEASE. PROPOSED MECHANISM OF ACTION BASED ON STRUCTURE OF ENZYME-THYMIDINE 3(PRIME),5(PRIME)-BIPHOSPHATE-CALCIUM ION COMPLEX AT 1.5-ANGSTROMS RESOLUTION" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/2SOB", "evidence_id": "EV-PDB-2SOB-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "2SOB", "doi": "10.1006/jmbi.1995.0365", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/2SOB" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "SN-OB, OB-FOLD SUB-DOMAIN OF STAPHYLOCOCCAL NUCLEASE, NMR, 10 STRUCTURES" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3BDC", "evidence_id": "EV-PDB-3BDC-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "3BDC", "doi": "10.1002/prot.22470", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3BDC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3D4D", "evidence_id": "EV-PDB-3D4D-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "3D4D", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3D4D" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3E5S", "evidence_id": "EV-PDB-3E5S-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "3E5S", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3E5S" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L103K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MPD", "PO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3EJI", "evidence_id": "EV-PDB-3EJI-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "3EJI", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3EJI" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L36K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3ERO", "evidence_id": "EV-PDB-3ERO-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "3ERO", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3ERO" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I72E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3ERQ", "evidence_id": "EV-PDB-3ERQ-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "3ERQ", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3ERQ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant L25K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3EVQ", "evidence_id": "EV-PDB-3EVQ-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "3EVQ", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3EVQ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "GOL", "MPD", "MRD", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A,B,C,D)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3HEJ", "evidence_id": "EV-PDB-3HEJ-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "3HEJ", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3HEJ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62R at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Nuclease A (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3ITP", "evidence_id": "EV-PDB-3ITP-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.75 }, "provenance": { "deposit_id": "3ITP", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3ITP" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS F34K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3MEH", "evidence_id": "EV-PDB-3MEH-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.5 }, "provenance": { "deposit_id": "3MEH", "doi": "10.1073/pnas.1200915109", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3MEH" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3MHB", "evidence_id": "EV-PDB-3MHB-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.55 }, "provenance": { "deposit_id": "3MHB", "doi": "10.1073/pnas.1200915109", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3MHB" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L38A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3MXP", "evidence_id": "EV-PDB-3MXP-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.61 }, "provenance": { "deposit_id": "3MXP", "doi": "10.1073/pnas.1200915109", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3MXP" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3MZ5", "evidence_id": "EV-PDB-3MZ5-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.58 }, "provenance": { "deposit_id": "3MZ5", "doi": "10.1073/pnas.1200915109", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3MZ5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L103A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Nuclease A (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3NHH", "evidence_id": "EV-PDB-3NHH-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "3NHH", "doi": "10.1073/pnas.1402900111", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3NHH" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant V23E-L36K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Nuclease A (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3NK9", "evidence_id": "EV-PDB-3NK9-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "3NK9", "doi": "10.1073/pnas.1200915109", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3NK9" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V74A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3NP8", "evidence_id": "EV-PDB-3NP8-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "3NP8", "doi": "10.1073/pnas.1200915109", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3NP8" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L36A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3NQT", "evidence_id": "EV-PDB-3NQT-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "3NQT", "doi": "10.1073/pnas.1200915109", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3NQT" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 5.0 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3NUC", "evidence_id": "EV-PDB-3NUC-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "3NUC", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3NUC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STAPHLOCOCCAL NUCLEASE, 1-N-PROPANE THIOL DISULFIDE TO V23C VARIANT" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3NXW", "evidence_id": "EV-PDB-3NXW-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "3NXW", "doi": "10.1073/pnas.1200915109", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3NXW" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L125A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3OSO", "evidence_id": "EV-PDB-3OSO-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "3OSO", "doi": "10.1073/pnas.1200915109", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3OSO" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3P1H", "evidence_id": "EV-PDB-3P1H-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.79 }, "provenance": { "deposit_id": "3P1H", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3P1H" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/I92A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3PMF", "evidence_id": "EV-PDB-3PMF-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "3PMF", "doi": "10.1073/pnas.1200915109", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3PMF" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3QB3", "evidence_id": "EV-PDB-3QB3-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.63 }, "provenance": { "deposit_id": "3QB3", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3QB3" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92KL25A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3SHL", "evidence_id": "EV-PDB-3SHL-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.48 }, "provenance": { "deposit_id": "3SHL", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3SHL" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V74KL25A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3SK4", "evidence_id": "EV-PDB-3SK4-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "3SK4", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3SK4" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N/V23E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3SK5", "evidence_id": "EV-PDB-3SK5-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "3SK5", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3SK5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V39D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MRD", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3SK6", "evidence_id": "EV-PDB-3SK6-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "3SK6", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3SK6" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23K/L36E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MPD", "PO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3SK8", "evidence_id": "EV-PDB-3SK8-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "3SK8", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3SK8" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS M98G apo protein at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3SR1", "evidence_id": "EV-PDB-3SR1-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.45 }, "provenance": { "deposit_id": "3SR1", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3SR1" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G bound to Ca2+ and thymidine-5',3'-diphosphate at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3S9W", "evidence_id": "EV-PDB-3S9W-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "3S9W", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3S9W" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS M98G bound to Ca2+ and thymidine-5',3'-diphosphate at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3RUZ", "evidence_id": "EV-PDB-3RUZ-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.58 }, "provenance": { "deposit_id": "3RUZ", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3RUZ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V74K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.5, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3P75", "evidence_id": "EV-PDB-3P75-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "3P75", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3P75" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V104D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "PO4", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3OWF", "evidence_id": "EV-PDB-3OWF-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "3OWF", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3OWF" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66R at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "PO4", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3LX0", "evidence_id": "EV-PDB-3LX0-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.5 }, "provenance": { "deposit_id": "3LX0", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3LX0" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3HZX", "evidence_id": "EV-PDB-3HZX-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "3HZX", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3HZX" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of Staphylococcal nuclease variant D+PHS/V66K at pH 9 determined at 100 K" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3H6M", "evidence_id": "EV-PDB-3H6M-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "3H6M", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3H6M" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MPD", "PO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3DMU", "evidence_id": "EV-PDB-3DMU-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "3DMU", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3DMU" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant PHS T62K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3DHQ", "evidence_id": "EV-PDB-3DHQ-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.15 }, "provenance": { "deposit_id": "3DHQ", "doi": "10.1073/pnas.1104808108", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3DHQ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS A90R at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3D6C", "evidence_id": "EV-PDB-3D6C-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "3D6C", "doi": "10.1016/j.jmb.2009.03.039", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3D6C" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant PHS L38E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3C1F", "evidence_id": "EV-PDB-3C1F-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "3C1F", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3C1F" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V104K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3C1E", "evidence_id": "EV-PDB-3C1E-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "3C1E", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3C1E" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L125K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3SXH", "evidence_id": "EV-PDB-3SXH-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.4 }, "provenance": { "deposit_id": "3SXH", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3SXH" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92AL103A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "MRD", "PO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3T13", "evidence_id": "EV-PDB-3T13-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "3T13", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3T13" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS A69G at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3T16", "evidence_id": "EV-PDB-3T16-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "3T16", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3T16" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant NVIAGA/M98G at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MRD", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3TME", "evidence_id": "EV-PDB-3TME-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.4 }, "provenance": { "deposit_id": "3TME", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3TME" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MPD", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3TP5", "evidence_id": "EV-PDB-3TP5-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "3TP5", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3TP5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+NVIAGLA V23E/L36E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3TP6", "evidence_id": "EV-PDB-3TP6-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "3TP6", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3TP6" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/L103K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3TP7", "evidence_id": "EV-PDB-3TP7-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "3TP7", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3TP7" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V99E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "PO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3U9O", "evidence_id": "EV-PDB-3U9O-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.95 }, "provenance": { "deposit_id": "3U9O", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3U9O" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant NVIAGA/N100G at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3V2T", "evidence_id": "EV-PDB-3V2T-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "3V2T", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3V2T" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V66A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3VA5", "evidence_id": "EV-PDB-3VA5-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.55 }, "provenance": { "deposit_id": "3VA5", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3VA5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V23A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4DFA", "evidence_id": "EV-PDB-4DFA-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.404 }, "provenance": { "deposit_id": "4DFA", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4DFA" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L36A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4DGZ", "evidence_id": "EV-PDB-4DGZ-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.47 }, "provenance": { "deposit_id": "4DGZ", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4DGZ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L125A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4DU9", "evidence_id": "EV-PDB-4DU9-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.63 }, "provenance": { "deposit_id": "4DU9", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4DU9" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/V74A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "MPD", "PO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4E6I", "evidence_id": "EV-PDB-4E6I-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "4E6I", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4E6I" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant NVIAGA V99G at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4EQN", "evidence_id": "EV-PDB-4EQN-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "4EQN", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4EQN" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/I72K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4EQO", "evidence_id": "EV-PDB-4EQO-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.697 }, "provenance": { "deposit_id": "4EQO", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4EQO" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V99D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "PO4" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4EVO", "evidence_id": "EV-PDB-4EVO-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.95 }, "provenance": { "deposit_id": "4EVO", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4EVO" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant NVIAGA/E122G at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4F8M", "evidence_id": "EV-PDB-4F8M-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "4F8M", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4F8M" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23I/I92V at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4HMI", "evidence_id": "EV-PDB-4HMI-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.75 }, "provenance": { "deposit_id": "4HMI", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4HMI" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V99K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4HMJ", "evidence_id": "EV-PDB-4HMJ-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.35 }, "provenance": { "deposit_id": "4HMJ", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4HMJ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L36D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4HTH", "evidence_id": "EV-PDB-4HTH-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.75 }, "provenance": { "deposit_id": "4HTH", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4HTH" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+VIAGLA at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4IAL", "evidence_id": "EV-PDB-4IAL-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.595 }, "provenance": { "deposit_id": "4IAL", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4IAL" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS H121E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4ID6", "evidence_id": "EV-PDB-4ID6-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.898 }, "provenance": { "deposit_id": "4ID6", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4ID6" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of Staphylococcal nuclease mutant V23I/I72L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "MPD", "PO4", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4IUN", "evidence_id": "EV-PDB-4IUN-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "4IUN", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4IUN" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS R126E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4IZ8", "evidence_id": "EV-PDB-4IZ8-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "4IZ8", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4IZ8" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS H8E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4J1M", "evidence_id": "EV-PDB-4J1M-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "4J1M", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4J1M" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS R105E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4J6H", "evidence_id": "EV-PDB-4J6H-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "4J6H", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4J6H" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS H121Q at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.5, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4K14", "evidence_id": "EV-PDB-4K14-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.601 }, "provenance": { "deposit_id": "4K14", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4K14" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of Staphylococcal nuclease mutant V66I/V99L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4K2K", "evidence_id": "EV-PDB-4K2K-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "4K2K", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4K2K" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A/L36A/I92A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4K2L", "evidence_id": "EV-PDB-4K2L-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.55 }, "provenance": { "deposit_id": "4K2L", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4K2L" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92Q at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4K5V", "evidence_id": "EV-PDB-4K5V-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.75 }, "provenance": { "deposit_id": "4K5V", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4K5V" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/L36A/V99T at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "MRD", "PO4", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4K5W", "evidence_id": "EV-PDB-4K5W-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "4K5W", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4K5W" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant V23M/L25F/T62F at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "MRD", "PO4", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4K5X", "evidence_id": "EV-PDB-4K5X-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "4K5X", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4K5X" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant V23M/L36F at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "MPD", "PO4", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4K6D", "evidence_id": "EV-PDB-4K6D-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "4K6D", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4K6D" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant V23M/T62F at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4K8I", "evidence_id": "EV-PDB-4K8I-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "4K8I", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4K8I" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of Staphylococcal Nuclease mutant I92V/V99L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4KD3", "evidence_id": "EV-PDB-4KD3-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.75 }, "provenance": { "deposit_id": "4KD3", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4KD3" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L25A/V66A/I92A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "MRD", "PO4", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4KD4", "evidence_id": "EV-PDB-4KD4-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.55 }, "provenance": { "deposit_id": "4KD4", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4KD4" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant V23L/L25V/V66I/I72V at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4KHV", "evidence_id": "EV-PDB-4KHV-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "4KHV", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4KHV" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23S at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4KJN", "evidence_id": "EV-PDB-4KJN-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.55 }, "provenance": { "deposit_id": "4KJN", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4KJN" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V66A/V99T at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4KJO", "evidence_id": "EV-PDB-4KJO-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "4KJO", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4KJO" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L36A/V66T/V99T at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4KTA", "evidence_id": "EV-PDB-4KTA-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.95 }, "provenance": { "deposit_id": "4KTA", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4KTA" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS R105Q at cryogenic temperatures" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4KV6", "evidence_id": "EV-PDB-4KV6-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.55 }, "provenance": { "deposit_id": "4KV6", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4KV6" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS R126Q at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4K8J", "evidence_id": "EV-PDB-4K8J-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "4K8J", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4K8J" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of Staphylococcal nuclease mutant V23L/V66I" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4I65", "evidence_id": "EV-PDB-4I65-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.61 }, "provenance": { "deposit_id": "4I65", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4I65" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of Staphylococcal nuclease mutant V23I/L25V/I72V/I92V" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4H7B", "evidence_id": "EV-PDB-4H7B-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "4H7B", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4H7B" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of Staphylococcal nuclease mutant I72V/V99L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.4, "salt_mM": null, "temperature_c": 26.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4G57", "evidence_id": "EV-PDB-4G57-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.81 }, "provenance": { "deposit_id": "4G57", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4G57" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Staphylococcal Nuclease double mutant I72L, I92L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4F7X", "evidence_id": "EV-PDB-4F7X-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "4F7X", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4F7X" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92A/L25A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4EQP", "evidence_id": "EV-PDB-4EQP-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.35 }, "provenance": { "deposit_id": "4EQP", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4EQP" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4DF7", "evidence_id": "EV-PDB-4DF7-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.5 }, "provenance": { "deposit_id": "4DF7", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4DF7" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23L/V99I at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/3TP8", "evidence_id": "EV-PDB-3TP8-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "3TP8", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/3TP8" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4KY5", "evidence_id": "EV-PDB-4KY5-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.4 }, "provenance": { "deposit_id": "4KY5", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4KY5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D at pH 7 and cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4KY6", "evidence_id": "EV-PDB-4KY6-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "4KY6", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4KY6" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L25D at pH 6 and cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4KY7", "evidence_id": "EV-PDB-4KY7-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.55 }, "provenance": { "deposit_id": "4KY7", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4KY7" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V74D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4LAA", "evidence_id": "EV-PDB-4LAA-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.58 }, "provenance": { "deposit_id": "4LAA", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4LAA" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L36H at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4ME5", "evidence_id": "EV-PDB-4ME5-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "4ME5", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4ME5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23S/V66A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4MIU", "evidence_id": "EV-PDB-4MIU-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.67 }, "provenance": { "deposit_id": "4MIU", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4MIU" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V99T at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4N9P", "evidence_id": "EV-PDB-4N9P-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.4 }, "provenance": { "deposit_id": "4N9P", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4N9P" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/L25A/V99T at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4N9T", "evidence_id": "EV-PDB-4N9T-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "4N9T", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4N9T" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92S at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4NDX", "evidence_id": "EV-PDB-4NDX-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.496 }, "provenance": { "deposit_id": "4NDX", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4NDX" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4NKL", "evidence_id": "EV-PDB-4NKL-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "4NKL", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4NKL" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92Q at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4NMZ", "evidence_id": "EV-PDB-4NMZ-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "4NMZ", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4NMZ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23Q/V66A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4NP5", "evidence_id": "EV-PDB-4NP5-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.5 }, "provenance": { "deposit_id": "4NP5", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4NP5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66A/I92N at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "PO4", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4ODG", "evidence_id": "EV-PDB-4ODG-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.73 }, "provenance": { "deposit_id": "4ODG", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4ODG" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant V23I/V66I/V74I/V99I at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4OL7", "evidence_id": "EV-PDB-4OL7-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.67 }, "provenance": { "deposit_id": "4OL7", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4OL7" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E A109E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4PMB", "evidence_id": "EV-PDB-4PMB-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "4PMB", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4PMB" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylcoccal nuclease variant Delta+PHS I92S at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4PMC", "evidence_id": "EV-PDB-4PMC-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "4PMC", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4PMC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K V74K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4PNY", "evidence_id": "EV-PDB-4PNY-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.68 }, "provenance": { "deposit_id": "4PNY", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4PNY" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I72H at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 19.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4QB4", "evidence_id": "EV-PDB-4QB4-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "4QB4", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4QB4" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal Structure of Staphylococcal Nuclease mutant V23L/L25V/V66L" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "PO4", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4QF4", "evidence_id": "EV-PDB-4QF4-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "4QF4", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4QF4" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant V23M at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "MRD", "PO4", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4R8N", "evidence_id": "EV-PDB-4R8N-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "4R8N", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4R8N" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant V23I/V66I/I72V/I92V at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": 3.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4RKB", "evidence_id": "EV-PDB-4RKB-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.88 }, "provenance": { "deposit_id": "4RKB", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4RKB" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66T/V99T at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4RKL", "evidence_id": "EV-PDB-4RKL-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.66 }, "provenance": { "deposit_id": "4RKL", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4RKL" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T/V66T at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4S3S", "evidence_id": "EV-PDB-4S3S-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.64 }, "provenance": { "deposit_id": "4S3S", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4S3S" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92K/V23A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4TRD", "evidence_id": "EV-PDB-4TRD-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "4TRD", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4TRD" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylcoccal nuclease variant Delta+PHS I72K/V74K at pH 9.0 at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4WOR", "evidence_id": "EV-PDB-4WOR-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.596 }, "provenance": { "deposit_id": "4WOR", "doi": "10.1073/pnas.94.12.6180", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4WOR" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Staphylococcal nuclease in complex with Ca2+ and thymidine-3'-5'-diphosphate (pdTp) at room temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Nuclease A (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4YIJ", "evidence_id": "EV-PDB-4YIJ-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.64 }, "provenance": { "deposit_id": "4YIJ", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4YIJ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylcoccal nuclease variant Delta+PHS A109E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4ZQ3", "evidence_id": "EV-PDB-4ZQ3-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "4ZQ3", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4ZQ3" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92T at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4ZUI", "evidence_id": "EV-PDB-4ZUI-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "4ZUI", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4ZUI" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23H at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/4ZUJ", "evidence_id": "EV-PDB-4ZUJ-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.72 }, "provenance": { "deposit_id": "4ZUJ", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/4ZUJ" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91H at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5C3W", "evidence_id": "EV-PDB-5C3W-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.72 }, "provenance": { "deposit_id": "5C3W", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5C3W" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5C3X", "evidence_id": "EV-PDB-5C3X-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.95 }, "provenance": { "deposit_id": "5C3X", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5C3X" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I72D/N100E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5C4H", "evidence_id": "EV-PDB-5C4H-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "5C4H", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5C4H" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92H at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5C4Z", "evidence_id": "EV-PDB-5C4Z-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.62 }, "provenance": { "deposit_id": "5C4Z", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5C4Z" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L25H at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "PO4", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5C5L", "evidence_id": "EV-PDB-5C5L-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.75 }, "provenance": { "deposit_id": "5C5L", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5C5L" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E / L125E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5C5M", "evidence_id": "EV-PDB-5C5M-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "5C5M", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5C5M" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D / V104E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5C6A", "evidence_id": "EV-PDB-5C6A-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "5C6A", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5C6A" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L25E / N100E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5CC4", "evidence_id": "EV-PDB-5CC4-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "5CC4", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5CC4" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5CGK", "evidence_id": "EV-PDB-5CGK-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "5CGK", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5CGK" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66D/N100E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5CR3", "evidence_id": "EV-PDB-5CR3-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "5CR3", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5CR3" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V104E/L125E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5CV4", "evidence_id": "EV-PDB-5CV4-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "5CV4", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5CV4" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/V66H at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5CV5", "evidence_id": "EV-PDB-5CV5-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "5CV5", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5CV5" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS K64G/V66K/E67G at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5CV6", "evidence_id": "EV-PDB-5CV6-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.95 }, "provenance": { "deposit_id": "5CV6", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5CV6" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5CV7", "evidence_id": "EV-PDB-5CV7-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "5CV7", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5CV7" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I92D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5CV8", "evidence_id": "EV-PDB-5CV8-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.58 }, "provenance": { "deposit_id": "5CV8", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5CV8" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/T62H at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5CV9", "evidence_id": "EV-PDB-5CV9-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.62 }, "provenance": { "deposit_id": "5CV9", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5CV9" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L36E/V66H at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5DAU", "evidence_id": "EV-PDB-5DAU-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.5 }, "provenance": { "deposit_id": "5DAU", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5DAU" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V39E/V104E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5DEH", "evidence_id": "EV-PDB-5DEH-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "5DEH", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5DEH" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS N100D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5E1F", "evidence_id": "EV-PDB-5E1F-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "5E1F", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5E1F" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS G20E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5E3F", "evidence_id": "EV-PDB-5E3F-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.75 }, "provenance": { "deposit_id": "5E3F", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5E3F" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5EGT", "evidence_id": "EV-PDB-5EGT-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "5EGT", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5EGT" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5EKL", "evidence_id": "EV-PDB-5EKL-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "5EKL", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5EKL" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D/N100E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5F2D", "evidence_id": "EV-PDB-5F2D-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "5F2D", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5F2D" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92N/V99T at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5HUR", "evidence_id": "EV-PDB-5HUR-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.5 }, "provenance": { "deposit_id": "5HUR", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5HUR" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L25T/I92K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5I6W", "evidence_id": "EV-PDB-5I6W-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "5I6W", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5I6W" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS A58D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5I6Y", "evidence_id": "EV-PDB-5I6Y-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "5I6Y", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5I6Y" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS A90E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5IOC", "evidence_id": "EV-PDB-5IOC-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "5IOC", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5IOC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5ISR", "evidence_id": "EV-PDB-5ISR-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "5ISR", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5ISR" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L38E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5J1Z", "evidence_id": "EV-PDB-5J1Z-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.73 }, "provenance": { "deposit_id": "5J1Z", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5J1Z" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS A90D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5J22", "evidence_id": "EV-PDB-5J22-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "5J22", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5J22" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L103D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5JAV", "evidence_id": "EV-PDB-5JAV-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.895 }, "provenance": { "deposit_id": "5JAV", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5JAV" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS Y91D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5JOB", "evidence_id": "EV-PDB-5JOB-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.45 }, "provenance": { "deposit_id": "5JOB", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5JOB" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92A at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -173.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5KEE", "evidence_id": "EV-PDB-5KEE-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.5 }, "provenance": { "deposit_id": "5KEE", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5KEE" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L25K/I92F at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "PO4", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5KRU", "evidence_id": "EV-PDB-5KRU-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "5KRU", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5KRU" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62E/V66K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5KYI", "evidence_id": "EV-PDB-5KYI-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "5KYI", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5KYI" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 9 at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.15, "salt_mM": null, "temperature_c": 5.0 }, "construct": { "description": "STAPHYLOCOCCAL NUCLEASE (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5NUC", "evidence_id": "EV-PDB-5NUC-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "5NUC", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5NUC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "STAPHYLOCOCCAL NUCLEASE, 1-N-PENTANE THIOL DISULFIDE TO V23C VARIANT" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6B8R", "evidence_id": "EV-PDB-6B8R-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.65 }, "provenance": { "deposit_id": "6B8R", "doi": "10.1021/acs.jpcb.7b12121", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6B8R" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23K/L36Q at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6EEG", "evidence_id": "EV-PDB-6EEG-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.95 }, "provenance": { "deposit_id": "6EEG", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6EEG" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylcoccal nuclease variant Delta+PHS T62E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -196.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6OK8", "evidence_id": "EV-PDB-6OK8-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "6OK8", "doi": "10.1107/S2059798319013226", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6OK8" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS K127L at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -196.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6U0X", "evidence_id": "EV-PDB-6U0X-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.86 }, "provenance": { "deposit_id": "6U0X", "doi": "10.1107/S2059798319013226", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6U0X" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS Q123D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6XSB", "evidence_id": "EV-PDB-6XSB-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.95 }, "provenance": { "deposit_id": "6XSB", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6XSB" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS A132S at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6XSC", "evidence_id": "EV-PDB-6XSC-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "6XSC", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6XSC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23T at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6XSE", "evidence_id": "EV-PDB-6XSE-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.1 }, "provenance": { "deposit_id": "6XSE", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6XSE" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS F34Y at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6XSF", "evidence_id": "EV-PDB-6XSF-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "6XSF", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6XSF" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T41V at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6XSG", "evidence_id": "EV-PDB-6XSG-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "6XSG", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6XSG" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66T at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6XSH", "evidence_id": "EV-PDB-6XSH-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "6XSH", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6XSH" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62V at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6XSI", "evidence_id": "EV-PDB-6XSI-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.0 }, "provenance": { "deposit_id": "6XSI", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6XSI" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V104T at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 36.85 }, "construct": { "description": "Disordered protein(D131D) (chains B)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8K2R", "evidence_id": "EV-PDB-8K2R-2", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "8K2R", "doi": "10.1038/s41594-024-01337-z", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8K2R" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "The structure of HtpG M domain in complex with unstructured D131D binding site b" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": 36.85 }, "construct": { "description": "Nuclease A (chains C)", "labels": [] }, "data_format": "mmCIF + NMR restraints", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/8K2T", "evidence_id": "EV-PDB-8K2T-2", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "structure", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "8K2T", "doi": "10.1038/s41594-024-01337-z", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/8K2T" }, "reader": null, "source_kind": "deposit", "technique": "nmr_structure", "title": "Solution structure of full-length HtpG in complex with D131D" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Nuclease A (chains A,B)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9C11", "evidence_id": "EV-PDB-9C11-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.95 }, "provenance": { "deposit_id": "9C11", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9C11" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS L36R at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/9CU9", "evidence_id": "EV-PDB-9CU9-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "9CU9", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/9CU9" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23D/L36K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -196.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6U0W", "evidence_id": "EV-PDB-6U0W-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "6U0W", "doi": "10.1107/S2059798319013226", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6U0W" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS K133M at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/6EEK", "evidence_id": "EV-PDB-6EEK-1", "mappings": [ { "confidence": "low", "justification": "Experimental structure without pdTp; variants (for example the Δ+PHS series) are still native folds.", "rule": "snase-apo-structures", "state_id": "SNase-apo", "who": "rule:snase-apo-structures" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 2.2 }, "provenance": { "deposit_id": "6EEK", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/6EEK" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of apo Staphylcoccal nuclease variant Delta+PHS T62E/V66K, pH 7 at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5KYL", "evidence_id": "EV-PDB-5KYL-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "5KYL", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5KYL" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62K/V66E pH 7 at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5KIX", "evidence_id": "EV-PDB-5KIX-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.75 }, "provenance": { "deposit_id": "5KIX", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5KIX" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I92E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5KGU", "evidence_id": "EV-PDB-5KGU-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.9 }, "provenance": { "deposit_id": "5KGU", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5KGU" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS N118D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 7.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5K5P", "evidence_id": "EV-PDB-5K5P-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.83 }, "provenance": { "deposit_id": "5K5P", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5K5P" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS I72K L103K at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5IOD", "evidence_id": "EV-PDB-5IOD-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.6 }, "provenance": { "deposit_id": "5IOD", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5IOD" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/V99E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5IIF", "evidence_id": "EV-PDB-5IIF-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.85 }, "provenance": { "deposit_id": "5IIF", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5IIF" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5IGG", "evidence_id": "EV-PDB-5IGG-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "5IGG", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5IGG" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V66H/I72E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 8.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5IGF", "evidence_id": "EV-PDB-5IGF-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.7 }, "provenance": { "deposit_id": "5IGF", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5IGF" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/V66D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5IGE", "evidence_id": "EV-PDB-5IGE-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "5IGE", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5IGE" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/V99D at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 9.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5IGD", "evidence_id": "EV-PDB-5IGD-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "5IGD", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5IGD" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS T62H/I92E at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; oligosaccharides appear as branched entities. pH and temperature are null when not deposited in machine-readable fields.", "ph": 6.0, "salt_mM": null, "temperature_c": -163.15 }, "construct": { "description": "Thermonuclease (chains A)", "labels": [] }, "data_format": "mmCIF + structure factors", "data_level": "processed", "data_link": "https://www.rcsb.org/structure/5IGC", "evidence_id": "EV-PDB-5IGC-1", "mappings": [ { "confidence": "high", "justification": "pdTp (ligand THP) bound in the active site.", "rule": "snase-pdtp-bound", "state_id": "SNase-pdTp", "who": "rule:snase-pdtp-bound" } ], "molecule_id": "P00644", "observable": { "type": "resolution", "uncertainty": null, "unit": "Å", "value": 1.8 }, "provenance": { "deposit_id": "5IGC", "doi": null, "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://www.rcsb.org/structure/5IGC" }, "reader": null, "source_kind": "deposit", "technique": "xray", "title": "Crystal structure of Staphylococcal nuclease variant Delta+PHS V23E/V66H at cryogenic temperature" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [ "CA", "THP" ], "notes": "Entry-level ligand list; 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"EV-BMRB-7011", "mappings": [], "molecule_id": "P00644", "observable": { "type": "chemical_shift", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "bmr7011", "doi": "10.13018/BMR7011", "licence": "CC0 1.0 (verify)", "retrieved": "2026-10-19", "source": "BMRB", "url": "https://bmrb.io/data_library/summary/index.php?bmrbId=7011" }, "reader": null, "source_kind": "deposit", "technique": "nmr_shifts", "title": "Solution structure of 97-109 segment of staphylococcal nuclease" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_vitro", "ions": [], "ligands": [], "notes": "From the first sample condition list of the entry.", "ph": 5.0, "salt_mM": null, "temperature_c": 24.85 }, "construct": { "description": "BMRB data: 13C chemical shifts, 1H chemical shifts", "labels": [] }, "data_format": "NMR-STAR", "data_level": "processed", "data_link": "https://bmrb.io/data_library/summary/index.php?bmrbId=7012", "evidence_id": "EV-BMRB-7012", "mappings": [], "molecule_id": "P00644", "observable": { "type": "chemical_shift", "uncertainty": null, "unit": null, "value": null }, "provenance": { "deposit_id": "bmr7012", "doi": "10.13018/BMR7012", "licence": "CC0 1.0 (verify)", "retrieved": "2026-10-19", "source": "BMRB", "url": "https://bmrb.io/data_library/summary/index.php?bmrbId=7012" }, "reader": null, "source_kind": "deposit", "technique": "nmr_shifts", "title": "Solution structure of 55-72 segment of staphylococcal nuclease" }, { "conditions": { "applied_force_pn": null, "buffer": null, "environment": "in_silico", "ions": [], "ligands": [], "notes": "Computed prediction; no experimental conditions.", "ph": null, "salt_mM": null, "temperature_c": null }, "construct": { "description": "AlphaFold Monomer v2.0 pipeline, residues 1-231", "labels": [] }, "data_format": "mmCIF, PAE JSON", "data_level": "processed", "data_link": "https://alphafold.ebi.ac.uk/entry/P00644", "evidence_id": "EV-AFDB-AF-P00644-F1", "mappings": [], "molecule_id": "P00644", "observable": { "type": "plddt", "uncertainty": null, "unit": "pLDDT", "value": 82.19 }, "provenance": { "deposit_id": "AF-P00644-F1", "doi": null, "licence": "CC BY 4.0", "retrieved": "2026-10-19", "source": "AlphaFold DB", "url": "https://alphafold.ebi.ac.uk/entry/P00644" }, "reader": null, "source_kind": "computed", "technique": "af_prediction", "title": "AlphaFold DB prediction AF-P00644-F1" } ], "models": [ { "assumptions": null, "basis": "observed", "constraints_used": [ "EV-PDB-1EY0-1" ], "file": "models/P00644/SNase-apo.cif", "format": "mmCIF", "model_id": "SNase-apo-cif", "molecule_id": "P00644", "provenance": { "deposit_id": "1EY0", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://files.rcsb.org/download/1EY0.cif" }, "state_id": "SNase-apo" }, { "assumptions": null, "basis": "observed", "constraints_used": [ "EV-PDB-1EY0-1" ], "file": "models/P00644/SNase-apo.pdb", "format": "PDB", "model_id": "SNase-apo-pdb", "molecule_id": "P00644", "provenance": { "deposit_id": "1EY0", "doi": "10.1006/jmbi.2000.4140", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://files.rcsb.org/download/1EY0.pdb" }, "state_id": "SNase-apo" }, { "assumptions": null, "basis": "observed", "constraints_used": [ "EV-PDB-1SNC-1" ], "file": "models/P00644/SNase-pdTp.cif", "format": "mmCIF", "model_id": "SNase-pdTp-cif", "molecule_id": "P00644", "provenance": { "deposit_id": "1SNC", "doi": "10.1002/prot.340050302", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://files.rcsb.org/download/1SNC.cif" }, "state_id": "SNase-pdTp" }, { "assumptions": null, "basis": "observed", "constraints_used": [ "EV-PDB-1SNC-1" ], "file": "models/P00644/SNase-pdTp.pdb", "format": "PDB", "model_id": "SNase-pdTp-pdb", "molecule_id": "P00644", "provenance": { "deposit_id": "1SNC", "doi": "10.1002/prot.340050302", "licence": "CC0 1.0 (wwPDB archive data)", "retrieved": "2026-10-19", "source": "RCSB PDB", "url": "https://files.rcsb.org/download/1SNC.pdb" }, "state_id": "SNase-pdTp" } ], "molecule": { "components": [], "domains": [], "gene": "nuc", "identifiers": { "genbank": null, "isoform": null, "nakb": null, "pdb": [], "rfam": null, "rnacentral": null, "uniprot": "P00644" }, "ligands": [ "Ca(2+)" ], "modifications": [], "molecule_id": "P00644", "molecule_type": "protein", "name": "Thermonuclease", "organism": { "name": "Staphylococcus aureus", "taxon_id": 1280 }, "provenance": { "deposit_id": "P00644", "doi": null, "licence": "CC BY 4.0 (UniProt)", "retrieved": "2026-10-19", "source": "UniProtKB", "url": "https://www.uniprot.org/uniprotkb/P00644/entry" }, "sequence": "MLVMTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCLVLTLVVVSSLSSSANASQTDNGVNRSGSEDPTVYSATSTKKLHKEPATLIKAIDGDTVKLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQRTDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQHLRKSEAQAKKEKLNIWSEDNADSGQ", "sequence_length": 231 }, "molecule_id": "P00644", "states": [ { "assumptions": [], "basis": "observed", "description": "Native fold of wild-type SNase without inhibitor, as in 1EY0 (1.6 Å).", "family_label": null, "molecule_id": "P00644", "name": "native, ligand-free", "parent_state": null, "population": null, "provenance": { "deposit_id": null, "doi": null, "licence": "Project licence (pending decision)", "retrieved": "2026-10-19", "source": "Conformome curation (P00644.yaml)", "url": "https://github.com/matth55/conformome/blob/HEAD/curation/P00644.yaml" }, "representative_model": "SNase-apo-cif", "secondary_structure": null, "state_id": "SNase-apo", "status": "draft" }, { "assumptions": [], "basis": "observed", "description": "Ternary complex with Ca2+ 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No observed structure.", "family_label": null, "molecule_id": "P00644", "name": "unfolded", "parent_state": null, "population": null, "provenance": { "deposit_id": null, "doi": null, "licence": "Project licence (pending decision)", "retrieved": "2026-10-19", "source": "Conformome curation (P00644.yaml)", "url": "https://github.com/matth55/conformome/blob/HEAD/curation/P00644.yaml" }, "representative_model": null, "secondary_structure": null, "state_id": "SNase-unfolded", "status": "draft" } ], "transitions": [] }